BIOCHEM ACS STUDY GUIDE Latest Update
2024-2025 Actual Exam from Credible Sources
with 130 Questions and Verified Correct Answers
Golden Ticket to Guaranteed A+ Verified by
Professor
_____ adds new nucleotides to the _____ end of the elongating strand during DNA
replication.
a. Polynucleotide phosphorylase; 3′
b. DNA polymerase: 3′
c. Sequenase; 5′
d. Replicase; 5′ - CORRECT ANSWER: B
________ acts as a structural disruptor in the middle of regular secondary structure
elements such as alpha helices and beta sheets.
a. Serine
b. Glycine
c. Cysteine
d. Methionine
e. Proline - CORRECT ANSWER: E
________ reads the 3' to 5' strand of DNA creating an RNA copy by adding nucleotides
to
the ______ end of the new RNA strand.
a. Transcriptase, 5'
b. RNA polymerase, 3'
c. RNAse, 5'
d. Polynucleotide phosphorylase, 3' - CORRECT ANSWER: B
,A BLAST search compares a sequence of __________ to a database of known
sequences.
[Q42]
a. Proteins
b. Proteins or Amino Acids
c. Nucleotides or Amino -acids
d. Amino Acids
e. Nucleotides - CORRECT ANSWER: C
A mixture of urease (pI = 5.1, mol. wt. 482,700), catalase (pI = 5.6 mol. wt. 247,500),
lactoglobin (pI 5.2, mol. wt. 37,100) and hemoglobin (pI 6.9, mol. wt. 64,500) were
applied in a pH 6.5 buffer to a DEAE-cellulose chromatography column and eluted with
the same buffer.
What was their order of elution?
A. urease, lactoglobin, catalase, hemoglobin
B. hemoglobin, catalase, lactoglobin, urease
C. urease, catalase, hemoglobin, lactoglobin
D. lactoglobin, hemoglobin, catalase, urease
E. cannot be determined from the information given - CORRECT ANSWER: B
A mixture of urease (pI = 5.1, mol. wt. 482,700), catalase (pI = 5.6, mol. wt. 247,500),
lactoglobin (pI 5.2, mol. wt. 37,100) and hemoglobin (pI 6.9, mol. wt. 64,500) were
applied in a
pH 6.5 buffer to a DEAE-cellulose chromatography column and eluted with the same
buffer.
What was their order of elution?
a. urease, lactoglobin, catalase, hemoglobin
b. lactoglobin, hemoglobin, catalase, urease
c. hemoglobin, catalase, lactoglobin, urease
,d. urease, catalase, hemoglobin, lactoglobin
e. cannot be determined from the information given - CORRECT ANSWER: C
A peptide was found to have a molecular mass of about 650 and upon hydrolysis
produced Ala, Cys, Lys, Phe, and Val in a 1:1:1:1:1 ratio. The peptide upon treatment
with Sanger's reagent produced DNP-Cys and exposure to carboxypeptidase produced
valine. Chymotrypsin treatment of the peptide produced a dipeptide that contained
sulfur and has a UV absorbance, and a tripeptide. Exposure of the peptide to trypsin
produced a dipeptide and a tripeptide. Deduce the sequence of the peptide.
A. Val-Ala-Lys-Phe-Cys
B. Cys-Lys-Phe-Ala-Val
C. Cys-Ala-Lys-Phe-Val
D. Cys-Phe-Lys-Ala-Val
E. Val-Phe-Lys-Ala-Cys - CORRECT ANSWER: D
A reagent commonly employed to cleave disulfide bonds in proteins is
A. 2,4-dinitrofluorobenzene
B. urea
C. 2-mercaptoethanol
D. phenylisothiocyanate
E. cyanogen bromide - CORRECT ANSWER: C
A size extraction column...
a. tags larger molecules for separation
b slows down smaller molecules, allowing larger ones to flow through more readily
c tags smaller molecules for separation
d slows down larger molecules, allowing smaller ones to flow through more easily -
CORRECT ANSWER: B
A β-D-aldopentofuranose has how many chiral carbons?
, A. 0
B. 2
C. 3
D. 4
E. 5 - CORRECT ANSWER: D
According to the Second Law of Thermodymnamics,
A. the total entropy change in any reaction that occurs spontaneously must be greater
than 0.
B. the total entropy change in any reaction that occurs spontaneously must be less
than 0.
C. the total entropy of the universe remains constant.
D. the total entropy of the universe is always decreasing. - CORRECT ANSWER: A
Acetyl CoA, the cytoplasmic substrate for fatty acid synthesis, is formed in mitochondria.
The
inner mitochondrial membrane is impermeable to acetyl CoA. Which of the following
compounds
is the form in which the carbon of acetyl CoA is transported to the cytoplasm?
a. Pyruvate
b. Acetate
c. Malate
d. Citrate
e. Glucose - CORRECT ANSWER: D
All of the following statements about the fluid mosaic model of biological membranes
are true
EXCEPT:
2024-2025 Actual Exam from Credible Sources
with 130 Questions and Verified Correct Answers
Golden Ticket to Guaranteed A+ Verified by
Professor
_____ adds new nucleotides to the _____ end of the elongating strand during DNA
replication.
a. Polynucleotide phosphorylase; 3′
b. DNA polymerase: 3′
c. Sequenase; 5′
d. Replicase; 5′ - CORRECT ANSWER: B
________ acts as a structural disruptor in the middle of regular secondary structure
elements such as alpha helices and beta sheets.
a. Serine
b. Glycine
c. Cysteine
d. Methionine
e. Proline - CORRECT ANSWER: E
________ reads the 3' to 5' strand of DNA creating an RNA copy by adding nucleotides
to
the ______ end of the new RNA strand.
a. Transcriptase, 5'
b. RNA polymerase, 3'
c. RNAse, 5'
d. Polynucleotide phosphorylase, 3' - CORRECT ANSWER: B
,A BLAST search compares a sequence of __________ to a database of known
sequences.
[Q42]
a. Proteins
b. Proteins or Amino Acids
c. Nucleotides or Amino -acids
d. Amino Acids
e. Nucleotides - CORRECT ANSWER: C
A mixture of urease (pI = 5.1, mol. wt. 482,700), catalase (pI = 5.6 mol. wt. 247,500),
lactoglobin (pI 5.2, mol. wt. 37,100) and hemoglobin (pI 6.9, mol. wt. 64,500) were
applied in a pH 6.5 buffer to a DEAE-cellulose chromatography column and eluted with
the same buffer.
What was their order of elution?
A. urease, lactoglobin, catalase, hemoglobin
B. hemoglobin, catalase, lactoglobin, urease
C. urease, catalase, hemoglobin, lactoglobin
D. lactoglobin, hemoglobin, catalase, urease
E. cannot be determined from the information given - CORRECT ANSWER: B
A mixture of urease (pI = 5.1, mol. wt. 482,700), catalase (pI = 5.6, mol. wt. 247,500),
lactoglobin (pI 5.2, mol. wt. 37,100) and hemoglobin (pI 6.9, mol. wt. 64,500) were
applied in a
pH 6.5 buffer to a DEAE-cellulose chromatography column and eluted with the same
buffer.
What was their order of elution?
a. urease, lactoglobin, catalase, hemoglobin
b. lactoglobin, hemoglobin, catalase, urease
c. hemoglobin, catalase, lactoglobin, urease
,d. urease, catalase, hemoglobin, lactoglobin
e. cannot be determined from the information given - CORRECT ANSWER: C
A peptide was found to have a molecular mass of about 650 and upon hydrolysis
produced Ala, Cys, Lys, Phe, and Val in a 1:1:1:1:1 ratio. The peptide upon treatment
with Sanger's reagent produced DNP-Cys and exposure to carboxypeptidase produced
valine. Chymotrypsin treatment of the peptide produced a dipeptide that contained
sulfur and has a UV absorbance, and a tripeptide. Exposure of the peptide to trypsin
produced a dipeptide and a tripeptide. Deduce the sequence of the peptide.
A. Val-Ala-Lys-Phe-Cys
B. Cys-Lys-Phe-Ala-Val
C. Cys-Ala-Lys-Phe-Val
D. Cys-Phe-Lys-Ala-Val
E. Val-Phe-Lys-Ala-Cys - CORRECT ANSWER: D
A reagent commonly employed to cleave disulfide bonds in proteins is
A. 2,4-dinitrofluorobenzene
B. urea
C. 2-mercaptoethanol
D. phenylisothiocyanate
E. cyanogen bromide - CORRECT ANSWER: C
A size extraction column...
a. tags larger molecules for separation
b slows down smaller molecules, allowing larger ones to flow through more readily
c tags smaller molecules for separation
d slows down larger molecules, allowing smaller ones to flow through more easily -
CORRECT ANSWER: B
A β-D-aldopentofuranose has how many chiral carbons?
, A. 0
B. 2
C. 3
D. 4
E. 5 - CORRECT ANSWER: D
According to the Second Law of Thermodymnamics,
A. the total entropy change in any reaction that occurs spontaneously must be greater
than 0.
B. the total entropy change in any reaction that occurs spontaneously must be less
than 0.
C. the total entropy of the universe remains constant.
D. the total entropy of the universe is always decreasing. - CORRECT ANSWER: A
Acetyl CoA, the cytoplasmic substrate for fatty acid synthesis, is formed in mitochondria.
The
inner mitochondrial membrane is impermeable to acetyl CoA. Which of the following
compounds
is the form in which the carbon of acetyl CoA is transported to the cytoplasm?
a. Pyruvate
b. Acetate
c. Malate
d. Citrate
e. Glucose - CORRECT ANSWER: D
All of the following statements about the fluid mosaic model of biological membranes
are true
EXCEPT:
