pKa (chapter 3) - ANSWER measure of the tendency of a group to give up a
proton (acidity); tendency decreases tenfold as pKa increases by one unit
thioester bond (1) - ANSWER compounds with functional group C-S-CoA-C
(eg, in acetyl-CoA)
zwitterion (3) - ANSWER dipolar ion with both positive and negative groups
but overall neutral charge; can act as either an acid or base
Isoelectric point - pI (chapter 3) - ANSWER the characteristic pH at which the
net electric charge is zero
SDS - Sodium Dodecyl Sulfate (chap 3) - ANSWER detergent used to unfold
proteins and give them uniform negative charge
SDS Page (3) - ANSWER chromatography used to separate proteins based on
mass. light proteins travel fast than heavier ones
PCR-Polymerase chain reaction (3)- ANSWER copies DNA multiple times to
increase sample size
, Isoelectric focusing (chapt 3)- ANSWER procedure used to determine the
isoelectric pt (pI) of a protein. Protein migrates through gel until pH = pI (net
charge = 0)
Two-Dimensional Electrophoresis (3)- ANSWER combines isoelectric focusing
and SDS electrophoresis; separates proteins by both molecular weight and pI
specific activity (3) - ANSWER number of enzyme units per mg of total
protein (a measure of enzyme purity)
activity (3) - ANSWER total units of a certain enzyme in a solution
Peptide bonds (chapter 4) - ANSWER C-N bond with double bond character
due to resonance (C-N bond cannot rotate, and is planar)
Edman degredation (3) - ANSWER used in the sequencing of polypeptides;
labels and removes ONLY the amino-residue from a polypeptide. carried out in
a machine called a sequenator
φ in peptide bonding (chapter 4) - ANSWER angle around the α-carbon -
amide nitrogen bond
ψ in peptide bonding (chapter 4) - ANSWER angle around the α-carbon -
carbonyl carbon bond
Ramachandran Plot (4) - ANSWER shows favoreable φ-ψ angle combinations.
3 main "wells" for α-helices, β-sheets, and left handed α-helices
proton (acidity); tendency decreases tenfold as pKa increases by one unit
thioester bond (1) - ANSWER compounds with functional group C-S-CoA-C
(eg, in acetyl-CoA)
zwitterion (3) - ANSWER dipolar ion with both positive and negative groups
but overall neutral charge; can act as either an acid or base
Isoelectric point - pI (chapter 3) - ANSWER the characteristic pH at which the
net electric charge is zero
SDS - Sodium Dodecyl Sulfate (chap 3) - ANSWER detergent used to unfold
proteins and give them uniform negative charge
SDS Page (3) - ANSWER chromatography used to separate proteins based on
mass. light proteins travel fast than heavier ones
PCR-Polymerase chain reaction (3)- ANSWER copies DNA multiple times to
increase sample size
, Isoelectric focusing (chapt 3)- ANSWER procedure used to determine the
isoelectric pt (pI) of a protein. Protein migrates through gel until pH = pI (net
charge = 0)
Two-Dimensional Electrophoresis (3)- ANSWER combines isoelectric focusing
and SDS electrophoresis; separates proteins by both molecular weight and pI
specific activity (3) - ANSWER number of enzyme units per mg of total
protein (a measure of enzyme purity)
activity (3) - ANSWER total units of a certain enzyme in a solution
Peptide bonds (chapter 4) - ANSWER C-N bond with double bond character
due to resonance (C-N bond cannot rotate, and is planar)
Edman degredation (3) - ANSWER used in the sequencing of polypeptides;
labels and removes ONLY the amino-residue from a polypeptide. carried out in
a machine called a sequenator
φ in peptide bonding (chapter 4) - ANSWER angle around the α-carbon -
amide nitrogen bond
ψ in peptide bonding (chapter 4) - ANSWER angle around the α-carbon -
carbonyl carbon bond
Ramachandran Plot (4) - ANSWER shows favoreable φ-ψ angle combinations.
3 main "wells" for α-helices, β-sheets, and left handed α-helices
