Buffer Order and Henderson Hasselbal - ANSWER Water, Buffer, Salt, Base
pH = pKa + log(A/HA)
pKa decreases as temp increases so pH decreases
Ramachandran plots - ANSWER Φ = N-R
ψ = C-R
optimal angle to prevent steric hindrance
Protein structure of a helix, B sheet, reverse turn - ANSWER a: LEMARKHQ
B: VICYFTW
RT: PSDNG
how many amino acids in alpha turn helix - ANSWER 3.6
Super secondary structure - ANSWER helix turn helix
parallel vs. antiparallel - ANSWER parallel wedges face same way
antiparallel face opposite
Isoelectric point (pI) - ANSWER pH at which a particular molecule carries no
net electrical charge.
, Protein Separation Techniques (ALL) - ANSWER Homogenization
Salting Out
Affinity Chromatgraphy
Ion Exchange
Size Exclusion/Gel Filtration
H, S, A, I, SG
He said all people SinG
Protein Separation Techniques (H) - ANSWER Homogenization
disrupt cells, sonicator, centrifuge
Protein Separation Techniques (S) - ANSWER Salting out
different proteins precipitate out at different salt concentrations
Protein Separation Techniques (A) - ANSWER Affinity chromatography
add a tag; histidine binds; see how well there is an affinity for the group
Protein Separation Techniques (I) - ANSWER ion exchange
separation based on charge; + proteins stick to - beads
SG Techniques of Protein Separation - ANSWER Size Exclusion/Gel Filtration
separation based on size
pH = pKa + log(A/HA)
pKa decreases as temp increases so pH decreases
Ramachandran plots - ANSWER Φ = N-R
ψ = C-R
optimal angle to prevent steric hindrance
Protein structure of a helix, B sheet, reverse turn - ANSWER a: LEMARKHQ
B: VICYFTW
RT: PSDNG
how many amino acids in alpha turn helix - ANSWER 3.6
Super secondary structure - ANSWER helix turn helix
parallel vs. antiparallel - ANSWER parallel wedges face same way
antiparallel face opposite
Isoelectric point (pI) - ANSWER pH at which a particular molecule carries no
net electrical charge.
, Protein Separation Techniques (ALL) - ANSWER Homogenization
Salting Out
Affinity Chromatgraphy
Ion Exchange
Size Exclusion/Gel Filtration
H, S, A, I, SG
He said all people SinG
Protein Separation Techniques (H) - ANSWER Homogenization
disrupt cells, sonicator, centrifuge
Protein Separation Techniques (S) - ANSWER Salting out
different proteins precipitate out at different salt concentrations
Protein Separation Techniques (A) - ANSWER Affinity chromatography
add a tag; histidine binds; see how well there is an affinity for the group
Protein Separation Techniques (I) - ANSWER ion exchange
separation based on charge; + proteins stick to - beads
SG Techniques of Protein Separation - ANSWER Size Exclusion/Gel Filtration
separation based on size
