BIO210 EXAMS QUESTIONS AND ANSWERS
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Where do you dispose of lab materials after you have used them (swabs, slides,
kimwipes, agar plates, etc.)?
-morgue
-biohazard waste bucket
What are some ways in which we practice aseptic technique in the lab?
-wear lab coats, eye googles, gloves
- lysol lab table before and after use
-flame loop before gathering specimens
-wash hands before and after lab
-put outerwear in cabinet
-open and close tube lids w/ one hand and same w/ plates
Why do you need to know the calibration of your microscope for each objective
lens?
the magnification and distance between each ocular unit in diff objective lens is different
so need to calibrate in order to get actual size of specimen
Under a microscope, how can you differentiate between a protozoan and a
bacterial cell? Algae and protozoa? Fungi and bacteria?
-Protozoa: bigger, organelles, nucleus, no cell walls
-Bacteria: smaller, no organelles, no nucleus, cell walls w/ peptidoglycan
-Alage: chloroplast that are light harvesting pigments, green b/c chorophyll pigment,
motile via dinoflagellates, cell wall w/ cellulose, multicellular or unicellular
-Protozoa: unicellular, no cell wall, motile via glidings, fagella, cilia, psuedopods
-fungi: bigger, organelles, nucleus, cell wall w/ chitin, non-motile, unicellular or
multicellular
-bacteria: some motile, unicellular, smaller, no organelles, no nucleus, cell wall w/
peptidoglycan
Algae: features + habitat
unicellular + multicellular, chloroplast organelles that are light haversting pigments that
can be utilized to carry out photosynthesis and use sunlight and CO2 to form glucose
and O2, some motile (dinoflagellates), cell walls w/ cellulose
Habitat: aquatic
Protozoa: features + habitat
unicellular, no cell walls, motile via glidings-pseudopods- flagella- cilia, some can
change between trophozoite and cyst form
Habitat: soil + aquatic
Fungi: features + habitat
, can be yeast( unicellular) or mold (multicellular), or dimorphic (both), cell wall w/ chitin,
reproduce by either spores (mold) or yeast (buds), non-motile
Habitat: warm + humid
Bacteria
unicellular, cell walls w/ peptidoglycan, reproduce via binary fission, vast metabolic
capacity, some motile, prok
Habitat: everywhere
What are some precautions that you must take when working with BSL-2
organisms? BSL-1 organisms?
BSL-1= work on lab bench or table, ppe worn as needed, have sink available, door that
separates lab from rest of facility
BSL-2= lab closed off while in session, ppe worn, procedures w/ aerosols or splashes
done under biological safety cabinet, wear face shield as needed, eye wash and sink
available, autoclave or any alternative decontamination waste basket for proper
disposal
Protozoa: Disease
Disease: giardiasis
Pathogen: giardia
Transmission: ingestion of water w/ pathogen, through contact from person to person,
and food contamination
symptoms: diarrhea, naseau , vomiting, bloating, new intolerance to diary, fatigue
Bacteria: Disease
Disease: cholera
Pathogen: vibrio cholera
Transmission: ingestion of contaminated water as cholera has a plasmid causes that
releases of a cholera toxin that disrupts the active transport in the chloride pumps
making the chloride move into the intestinal lumen causes the sodium ions to follow and
via osmosis water leaves the cells into the intestinal lumen
symptoms: diarrhea "rice water stools"
Algae: Disease
Disease: paralytic shellfish poisoning
Pathogen: psuedonitzchia
transmission: consuming contaminated fish and the neurotoxins lead to disabling of
nerve cell signals
symptoms: very rapid onset of memory loss, headaches, nausea, vomiting
Fungi
Disease: histoplasmosis
pathogen: histoplasma
transmission: inhaling spores of bird/bat droppings and
1. arm
2. fine adjustment knob/ coarse adjustment knob
3.base
4.eye piece
A+GRADED. Buy Quality Materials!
Where do you dispose of lab materials after you have used them (swabs, slides,
kimwipes, agar plates, etc.)?
-morgue
-biohazard waste bucket
What are some ways in which we practice aseptic technique in the lab?
-wear lab coats, eye googles, gloves
- lysol lab table before and after use
-flame loop before gathering specimens
-wash hands before and after lab
-put outerwear in cabinet
-open and close tube lids w/ one hand and same w/ plates
Why do you need to know the calibration of your microscope for each objective
lens?
the magnification and distance between each ocular unit in diff objective lens is different
so need to calibrate in order to get actual size of specimen
Under a microscope, how can you differentiate between a protozoan and a
bacterial cell? Algae and protozoa? Fungi and bacteria?
-Protozoa: bigger, organelles, nucleus, no cell walls
-Bacteria: smaller, no organelles, no nucleus, cell walls w/ peptidoglycan
-Alage: chloroplast that are light harvesting pigments, green b/c chorophyll pigment,
motile via dinoflagellates, cell wall w/ cellulose, multicellular or unicellular
-Protozoa: unicellular, no cell wall, motile via glidings, fagella, cilia, psuedopods
-fungi: bigger, organelles, nucleus, cell wall w/ chitin, non-motile, unicellular or
multicellular
-bacteria: some motile, unicellular, smaller, no organelles, no nucleus, cell wall w/
peptidoglycan
Algae: features + habitat
unicellular + multicellular, chloroplast organelles that are light haversting pigments that
can be utilized to carry out photosynthesis and use sunlight and CO2 to form glucose
and O2, some motile (dinoflagellates), cell walls w/ cellulose
Habitat: aquatic
Protozoa: features + habitat
unicellular, no cell walls, motile via glidings-pseudopods- flagella- cilia, some can
change between trophozoite and cyst form
Habitat: soil + aquatic
Fungi: features + habitat
, can be yeast( unicellular) or mold (multicellular), or dimorphic (both), cell wall w/ chitin,
reproduce by either spores (mold) or yeast (buds), non-motile
Habitat: warm + humid
Bacteria
unicellular, cell walls w/ peptidoglycan, reproduce via binary fission, vast metabolic
capacity, some motile, prok
Habitat: everywhere
What are some precautions that you must take when working with BSL-2
organisms? BSL-1 organisms?
BSL-1= work on lab bench or table, ppe worn as needed, have sink available, door that
separates lab from rest of facility
BSL-2= lab closed off while in session, ppe worn, procedures w/ aerosols or splashes
done under biological safety cabinet, wear face shield as needed, eye wash and sink
available, autoclave or any alternative decontamination waste basket for proper
disposal
Protozoa: Disease
Disease: giardiasis
Pathogen: giardia
Transmission: ingestion of water w/ pathogen, through contact from person to person,
and food contamination
symptoms: diarrhea, naseau , vomiting, bloating, new intolerance to diary, fatigue
Bacteria: Disease
Disease: cholera
Pathogen: vibrio cholera
Transmission: ingestion of contaminated water as cholera has a plasmid causes that
releases of a cholera toxin that disrupts the active transport in the chloride pumps
making the chloride move into the intestinal lumen causes the sodium ions to follow and
via osmosis water leaves the cells into the intestinal lumen
symptoms: diarrhea "rice water stools"
Algae: Disease
Disease: paralytic shellfish poisoning
Pathogen: psuedonitzchia
transmission: consuming contaminated fish and the neurotoxins lead to disabling of
nerve cell signals
symptoms: very rapid onset of memory loss, headaches, nausea, vomiting
Fungi
Disease: histoplasmosis
pathogen: histoplasma
transmission: inhaling spores of bird/bat droppings and
1. arm
2. fine adjustment knob/ coarse adjustment knob
3.base
4.eye piece
