GMS 6552 EXAM 2 QUESTIONS AND ANSWERS WITH
COMPLETE SOLUTIONS VERIFIED LATEST UPDATE
MODULE 5
An oncogene is defined as
a gene that causes or contributes to the development of cancer
What are the two types of evidence for oncogenic behavior?
In vivo
In vitro
What are the 4 criteria for In vivo oncogenic behavior
Cause cancer when activated in a transgenic animal
Render nontumorigenic cells tumorigenic
Knock down or knock out in tumorigenic cells renders the cells nontumorigenic
Activation/overexpression of the oncogene is strongly correlated with human cancer
What are the 3 criteria for In vitro oncogenic behavior
Causes anchorage-independent growth in soft agar (protection from anoikis)
Causes focus formation (loss of contact inhibition)
Renders cells growth factor independent
What is an example of a GTPase that plays a biochemical role in oncogenes.
Ras
What is an example of a Protein Kinases that plays a biochemical role in
oncogenes.
, Raf, Akt, BCR-ABL, Cdk4, Src
What is an example of a Lipid Kinases that plays a biochemical role in
oncogenes.
PI3-Kinase
What is an example of a Transcription factors that plays a biochemical role in
oncogenes.
Myc, Fos, Jun, E2F1, ....
What is an example of a Binding and inactivating apoptotic proteins that plays a
biochemical role in oncogenes.
BCL2
What is an example of a Protein Kinase Regulatory Subunits that plays a
biochemical role in oncogenes.
Cyclins A, E, and D
What is an example of a Transcriptional coactivators that plays a biochemical role
in oncogenes.
ß-Catenin, YAP, TAZ
Five Ways to Activate Oncogenes
1. Mutation of genes to make it overactive.
2. Amplification of a normal gene
3. Chromosomal Rearrangement
4. Promoter/enhancer insertion
5. Hypomethylation of oncogene
What are some examples of Chromosome Rearrangement? (2)
COMPLETE SOLUTIONS VERIFIED LATEST UPDATE
MODULE 5
An oncogene is defined as
a gene that causes or contributes to the development of cancer
What are the two types of evidence for oncogenic behavior?
In vivo
In vitro
What are the 4 criteria for In vivo oncogenic behavior
Cause cancer when activated in a transgenic animal
Render nontumorigenic cells tumorigenic
Knock down or knock out in tumorigenic cells renders the cells nontumorigenic
Activation/overexpression of the oncogene is strongly correlated with human cancer
What are the 3 criteria for In vitro oncogenic behavior
Causes anchorage-independent growth in soft agar (protection from anoikis)
Causes focus formation (loss of contact inhibition)
Renders cells growth factor independent
What is an example of a GTPase that plays a biochemical role in oncogenes.
Ras
What is an example of a Protein Kinases that plays a biochemical role in
oncogenes.
, Raf, Akt, BCR-ABL, Cdk4, Src
What is an example of a Lipid Kinases that plays a biochemical role in
oncogenes.
PI3-Kinase
What is an example of a Transcription factors that plays a biochemical role in
oncogenes.
Myc, Fos, Jun, E2F1, ....
What is an example of a Binding and inactivating apoptotic proteins that plays a
biochemical role in oncogenes.
BCL2
What is an example of a Protein Kinase Regulatory Subunits that plays a
biochemical role in oncogenes.
Cyclins A, E, and D
What is an example of a Transcriptional coactivators that plays a biochemical role
in oncogenes.
ß-Catenin, YAP, TAZ
Five Ways to Activate Oncogenes
1. Mutation of genes to make it overactive.
2. Amplification of a normal gene
3. Chromosomal Rearrangement
4. Promoter/enhancer insertion
5. Hypomethylation of oncogene
What are some examples of Chromosome Rearrangement? (2)
