GMS 6504 FINAL EXAM QUESTIONS AND ANSWERS WITH
COMPLETE SOLUTIONS VERIFIED
-partition coefficient log P in -0.4 to +5.6 range
-molar refractory from 40 to 130
-molecular weight from 160 to 500
-polar surface no greater than 140A
-number of atoms from 20 to 70s
improvements lipinski parameters
Hydrogen bond donor functional group
-NH2
LogP of hydrophobic molecules
High
Reason for smaller polar surface areas in CNS drugs
It helps the drug cross the blood brain barrier
Functional group often utilized for pro-drugs
-numerous catalytic esterases present in vivo to hydrolyze simple esters
Esters
Protein domain in easily druggable targets
ATP-binding domain
Effect of synthetic lethality on therapeutic index
,Increase
Enzyme causing synthetic lethality in BRCA2 mutation
PARP
Functional group placed on proteins by kinases
Phosphates
Sequence of the catalytic site of a kinase
GIGSAG
Gatekeeper residue in a kinase
Methionine
Target of Type 1 kinase inhibitors
Active kinases
Selectivity of allosteric kinase inhibitors
Greater selectivity towards the target enzyme
Resistance mechanism reducing Herceptin affinity
Epitope masking
Source for downloading protein crystal structures
PDB
Purpose of spheres in docking process
They tell the computer where we want molecules to dock to.
Purpose of grid in docking process
It defines the electrostatic environment to use for energy calculations.
Accuracy of docking with rigid vs flexible ligands
Flexible
,Calculation of grid score
It is the sum of the electrostatic score and the Van Der Waals score.
Grid score predicting best binding to a protein
-50
Immune cells responsible for killing cancer cells
Cytotoxic T Lymphocytes
Type of antibody with greater human sequence
Humanized
Target of Rituximab
CD20
Target of Gemtuzumab
CD33
Injection in dendritic cell vaccines
Cells
Tumor specificity of T-VEC
It can only replicate in tumor cells.
Mechanism for preparing CAR-T cells
Retroviral gene therapy
Solid forming different crystal structures
Polymorphic
Form of DSC with one pan used
Neither
X-axis of a thermogram
, Temperature
Reading melting point from thermogram peak
Beginning
Batch likely more pure based on melting point
Batch B
Mass behavior of unstable compound in thermogravic analysis
Increase, decrease, or stay the same
Chromatography where analyte binds to solid phase
Adsorption
Ways to increase resolution in chromatogram
Decreasing peak width and Increasing distance between peaks
Molecule eluting first in size exclusion chromatography
Largest
Stationary phase in anion exchange column
Cations
Peak characteristics proportional to analyte concentration
Both peak height and peak area
Fastest elution in reverse-phase chromatography
THF
Isocratic elution
Uses one mobile phase for the entire run
Type of mass spectrometer collecting ions
Trapping
COMPLETE SOLUTIONS VERIFIED
-partition coefficient log P in -0.4 to +5.6 range
-molar refractory from 40 to 130
-molecular weight from 160 to 500
-polar surface no greater than 140A
-number of atoms from 20 to 70s
improvements lipinski parameters
Hydrogen bond donor functional group
-NH2
LogP of hydrophobic molecules
High
Reason for smaller polar surface areas in CNS drugs
It helps the drug cross the blood brain barrier
Functional group often utilized for pro-drugs
-numerous catalytic esterases present in vivo to hydrolyze simple esters
Esters
Protein domain in easily druggable targets
ATP-binding domain
Effect of synthetic lethality on therapeutic index
,Increase
Enzyme causing synthetic lethality in BRCA2 mutation
PARP
Functional group placed on proteins by kinases
Phosphates
Sequence of the catalytic site of a kinase
GIGSAG
Gatekeeper residue in a kinase
Methionine
Target of Type 1 kinase inhibitors
Active kinases
Selectivity of allosteric kinase inhibitors
Greater selectivity towards the target enzyme
Resistance mechanism reducing Herceptin affinity
Epitope masking
Source for downloading protein crystal structures
PDB
Purpose of spheres in docking process
They tell the computer where we want molecules to dock to.
Purpose of grid in docking process
It defines the electrostatic environment to use for energy calculations.
Accuracy of docking with rigid vs flexible ligands
Flexible
,Calculation of grid score
It is the sum of the electrostatic score and the Van Der Waals score.
Grid score predicting best binding to a protein
-50
Immune cells responsible for killing cancer cells
Cytotoxic T Lymphocytes
Type of antibody with greater human sequence
Humanized
Target of Rituximab
CD20
Target of Gemtuzumab
CD33
Injection in dendritic cell vaccines
Cells
Tumor specificity of T-VEC
It can only replicate in tumor cells.
Mechanism for preparing CAR-T cells
Retroviral gene therapy
Solid forming different crystal structures
Polymorphic
Form of DSC with one pan used
Neither
X-axis of a thermogram
, Temperature
Reading melting point from thermogram peak
Beginning
Batch likely more pure based on melting point
Batch B
Mass behavior of unstable compound in thermogravic analysis
Increase, decrease, or stay the same
Chromatography where analyte binds to solid phase
Adsorption
Ways to increase resolution in chromatogram
Decreasing peak width and Increasing distance between peaks
Molecule eluting first in size exclusion chromatography
Largest
Stationary phase in anion exchange column
Cations
Peak characteristics proportional to analyte concentration
Both peak height and peak area
Fastest elution in reverse-phase chromatography
THF
Isocratic elution
Uses one mobile phase for the entire run
Type of mass spectrometer collecting ions
Trapping
