UF MCB3020 Exam 3 Questions &
Answers 100% Accurate!!
The primer used in Sanger sequencing _________. - ANSWERShas a nucleotide
sequence complementary to the 3' end of the region to be copied
To carry out Sanger sequencing a mixture is needed containing _________. -
ANSWERSsingle-stranded DNA, DNA polymerase, complementary DNA primer, and
four deoxyribonucleotides A,T,C,G
In DNA sequencing. When a dideoxyribonucleotide is added to the daughter strand,
replication of the strand ________. - ANSWERSstops
To sequence DNA by the Sanger method, the DNA must first be made in single-
stranded form.
DNA sequencing is a technique for determining the complete sequence of bases (i.e.,
As, Ts, Gs, and Cs) for a particular piece of DNA. Sanger sequencing is the most
popular method for DNA sequencing. T/F - ANSWERSTrue
In the Sanger method, examining the electrophoresis gel and reading from bottom to
top, one base at a time, gives the sequence of DNA. T/F - ANSWERSTrue
Arrange the following events in the proper sequence for gene cloning.
1 = Incorporate gene into bacterial plasmid.
2 = Isolate DNA from organism containing desired gene.
3 = Incorporate cloned gene into bacterial cells.
4 = Fragment DNA with restriction enzyme. - ANSWERS2, 4, 1, 3
The function of the enzyme ligase is to ________. - ANSWERSform covalent bonds
between cloned gene and plasmid
"Sticky ends" are ________. - ANSWERSsingle-stranded DNA sequences that are
generated by staggered cuts
Restriction enzymes cut DNA at random sites. T/F - ANSWERSFalse
Which of the following is not a characteristic of lipid A? - ANSWERSNeurotoxicity
All of the following are components of the mammalian host immune system EXCEPT
________.
leukocytes
phagocytes
antigens
, antibodies - ANSWERSantigens
Which is a primary reason that females are at greater risk of a urinary tract infection
than are males? - ANSWERSThe urethra is shorter in women; therefore, bacteria are
closer in proximity to the bladder.
Which of the following is considered to be a biological defense mechanism? -
ANSWERSNormal microbiota
One example of infectious disease transmission by a fomite is _______. - ANSWERSa
child developing a cold after playing with a toy that harbored rhinovirus
In a lichen, the fungal partner _________.
All of the choices are correct.
provides a substratum within which the alga grows
protects the alga from excess light intensities
provides water and minerals to the alga - ANSWERSAll of the choices are correct.
Blood cell development occurs in the bone marrow of mammals during the process of
________. - ANSWERShematopoesis
Which of the following must a pathogen possess in order for it to be successful at
causing infectious disease?
Ability to damage the host
All of the choices are correct.
Ability to adhere to, colonize, or invade the host
Ability to be transported initially to the host - ANSWERSAll of the choices are correct.
Bacteria within biofilms exchange _________.
nutrients
All of the choices are correct.
quorum-sensing molecules
plasmids - ANSWERSAll of the choices are correct.
Methanogens are always engaged in relationships with other microbes because
methane production requires _________. - ANSWERSinterspecies hydrogen transfer
Cervical mucus has antibacterial activity. T/F – ANSWERSTru Macrophages are
phagocytic cells. T/F - ANSWERSTrue
The skin surface is a very favorable environment for colonization by most
microorganisms. T/F - ANSWERSFalse
Which of the following features of a pathogen is not considered to be a virulence factor
when establishing infection within a host? - ANSWERSPigment production
Answers 100% Accurate!!
The primer used in Sanger sequencing _________. - ANSWERShas a nucleotide
sequence complementary to the 3' end of the region to be copied
To carry out Sanger sequencing a mixture is needed containing _________. -
ANSWERSsingle-stranded DNA, DNA polymerase, complementary DNA primer, and
four deoxyribonucleotides A,T,C,G
In DNA sequencing. When a dideoxyribonucleotide is added to the daughter strand,
replication of the strand ________. - ANSWERSstops
To sequence DNA by the Sanger method, the DNA must first be made in single-
stranded form.
DNA sequencing is a technique for determining the complete sequence of bases (i.e.,
As, Ts, Gs, and Cs) for a particular piece of DNA. Sanger sequencing is the most
popular method for DNA sequencing. T/F - ANSWERSTrue
In the Sanger method, examining the electrophoresis gel and reading from bottom to
top, one base at a time, gives the sequence of DNA. T/F - ANSWERSTrue
Arrange the following events in the proper sequence for gene cloning.
1 = Incorporate gene into bacterial plasmid.
2 = Isolate DNA from organism containing desired gene.
3 = Incorporate cloned gene into bacterial cells.
4 = Fragment DNA with restriction enzyme. - ANSWERS2, 4, 1, 3
The function of the enzyme ligase is to ________. - ANSWERSform covalent bonds
between cloned gene and plasmid
"Sticky ends" are ________. - ANSWERSsingle-stranded DNA sequences that are
generated by staggered cuts
Restriction enzymes cut DNA at random sites. T/F - ANSWERSFalse
Which of the following is not a characteristic of lipid A? - ANSWERSNeurotoxicity
All of the following are components of the mammalian host immune system EXCEPT
________.
leukocytes
phagocytes
antigens
, antibodies - ANSWERSantigens
Which is a primary reason that females are at greater risk of a urinary tract infection
than are males? - ANSWERSThe urethra is shorter in women; therefore, bacteria are
closer in proximity to the bladder.
Which of the following is considered to be a biological defense mechanism? -
ANSWERSNormal microbiota
One example of infectious disease transmission by a fomite is _______. - ANSWERSa
child developing a cold after playing with a toy that harbored rhinovirus
In a lichen, the fungal partner _________.
All of the choices are correct.
provides a substratum within which the alga grows
protects the alga from excess light intensities
provides water and minerals to the alga - ANSWERSAll of the choices are correct.
Blood cell development occurs in the bone marrow of mammals during the process of
________. - ANSWERShematopoesis
Which of the following must a pathogen possess in order for it to be successful at
causing infectious disease?
Ability to damage the host
All of the choices are correct.
Ability to adhere to, colonize, or invade the host
Ability to be transported initially to the host - ANSWERSAll of the choices are correct.
Bacteria within biofilms exchange _________.
nutrients
All of the choices are correct.
quorum-sensing molecules
plasmids - ANSWERSAll of the choices are correct.
Methanogens are always engaged in relationships with other microbes because
methane production requires _________. - ANSWERSinterspecies hydrogen transfer
Cervical mucus has antibacterial activity. T/F – ANSWERSTru Macrophages are
phagocytic cells. T/F - ANSWERSTrue
The skin surface is a very favorable environment for colonization by most
microorganisms. T/F - ANSWERSFalse
Which of the following features of a pathogen is not considered to be a virulence factor
when establishing infection within a host? - ANSWERSPigment production
