ASCP BOARD OF CERTIFICATION MLT MICROBIOLOGY
PRACTICE TEST
This 30x50 um ovum is most commonly observed by the microscopic exam of
transparent adhesive tape mounts of perianal skin from a children complaining of
nocturnal anal pruritus. What is the most likely presumptive ID? Hint it is a type of
worm
A: Cystoisospora belli
B: Necator americanus (aka: hookworm)
C: Enterobius vermicularis (aka: roundworm)
D: Trichuris trichiura (aka: whipworm)
*This is a level 2 question*
C
Which of the following is the most suitable specimen for the isolation of
Bordetella pertussis?
A: Nasopharyngeal swab
B: Bronchial washing
C: Wound aspirate
D: Throat swab
*This is a level 6 question*
A
Illustrated in the top photograph is a 6-day old colony with a gray-white cob-web
like mycelium growing on a brain-heart infusion agar incubated at 30 degrees C.
Not the rim of black pigmentation on the upper margin of the colony. An attempt
failed to convert the colony to a yeast form by using 35 degrees C incubation. The
H&E-stained tissue section of the lung lesion from which the specimen was taken
as might be observed by a pathologist, is illustrated in the bottom image.
Considering these observations, what is the ID of this isolate?
A: Histoplasma capsulatum
B: Sporothrix shenckii
C: Blastomyces dermatitidis
D: Coccidioides immitis
*This is a level 7 question*
D
You are working on a blood culture & after 24 hours of growth, there are small
gray, non-hemolytic colonies, resembling Enterococcus species. Gram stain of
the colonies indicate Gram-positive cocci in pairs & chains. The tech must set up
PRACTICE TEST
This 30x50 um ovum is most commonly observed by the microscopic exam of
transparent adhesive tape mounts of perianal skin from a children complaining of
nocturnal anal pruritus. What is the most likely presumptive ID? Hint it is a type of
worm
A: Cystoisospora belli
B: Necator americanus (aka: hookworm)
C: Enterobius vermicularis (aka: roundworm)
D: Trichuris trichiura (aka: whipworm)
*This is a level 2 question*
C
Which of the following is the most suitable specimen for the isolation of
Bordetella pertussis?
A: Nasopharyngeal swab
B: Bronchial washing
C: Wound aspirate
D: Throat swab
*This is a level 6 question*
A
Illustrated in the top photograph is a 6-day old colony with a gray-white cob-web
like mycelium growing on a brain-heart infusion agar incubated at 30 degrees C.
Not the rim of black pigmentation on the upper margin of the colony. An attempt
failed to convert the colony to a yeast form by using 35 degrees C incubation. The
H&E-stained tissue section of the lung lesion from which the specimen was taken
as might be observed by a pathologist, is illustrated in the bottom image.
Considering these observations, what is the ID of this isolate?
A: Histoplasma capsulatum
B: Sporothrix shenckii
C: Blastomyces dermatitidis
D: Coccidioides immitis
*This is a level 7 question*
D
You are working on a blood culture & after 24 hours of growth, there are small
gray, non-hemolytic colonies, resembling Enterococcus species. Gram stain of
the colonies indicate Gram-positive cocci in pairs & chains. The tech must set up
