BCH 451 TEST 2 EXAM QUESTIONS AND ANSWERS WITH
COMPLETE SOLUTIONS VERIFIED
Oxidoreductases
catalyze reactions involving redox change
Transferase
A-X + B <--> B-X + A
Hydrolase
break bond by adding water
lyase
adding a molecule to double bond without breaking it
isomerase
one reactant --> one product
Ligase
requires atp to drive reaction
[s]
concentration of substrate
Vmax
maximum velocity of reaction
Km
Substrate concentration at 1/2 Vmax
Does velocity of reaction ever actually reach zero?
, no when the concentration of substrate is [1/2] of Km then there is not enough to drive
the reaction
how to generate michaelis menten plot
data from a bunch of different progress curves (different concentrations of substrate)
negative delta G
spontaneous towards products
postive delta G
spontaneous towards
two ways to compare enzymes
one substrate with two enzymes
two substrates with one type of enzyme
Competitive inhibition
inhibitor binds free enzyme and competes with substrate for active site
uncompetitive inhibition
inhibitor binds the enzyme substrate complex
noncompetitive inhibition
inhibitor binds free enzyme and enzyme substrate complex
types of reversible reactions
competitive, uncompetitive, noncompetitive
irreversible inhibitors
inhibitor binds substrate after X leaves enzyme as leaving group
Methotrexate
COMPLETE SOLUTIONS VERIFIED
Oxidoreductases
catalyze reactions involving redox change
Transferase
A-X + B <--> B-X + A
Hydrolase
break bond by adding water
lyase
adding a molecule to double bond without breaking it
isomerase
one reactant --> one product
Ligase
requires atp to drive reaction
[s]
concentration of substrate
Vmax
maximum velocity of reaction
Km
Substrate concentration at 1/2 Vmax
Does velocity of reaction ever actually reach zero?
, no when the concentration of substrate is [1/2] of Km then there is not enough to drive
the reaction
how to generate michaelis menten plot
data from a bunch of different progress curves (different concentrations of substrate)
negative delta G
spontaneous towards products
postive delta G
spontaneous towards
two ways to compare enzymes
one substrate with two enzymes
two substrates with one type of enzyme
Competitive inhibition
inhibitor binds free enzyme and competes with substrate for active site
uncompetitive inhibition
inhibitor binds the enzyme substrate complex
noncompetitive inhibition
inhibitor binds free enzyme and enzyme substrate complex
types of reversible reactions
competitive, uncompetitive, noncompetitive
irreversible inhibitors
inhibitor binds substrate after X leaves enzyme as leaving group
Methotrexate
