BCH 451 FINAL EXAM QUESTIONS AND ANSWERS WITH
COMPLETE SOLUTIONS VERIFIED LATEST UPDATE
(1) Which of the following is true?
a. For a reaction with a negative delta G Keq is less than 1.
b. ATP can activate another molecule by transferring a Pi group to the target.
c. Enzymes increase the rate of reactions through lowering delta G0.
d. The driving force of the hydrophobic effect is attraction between hydrophobic
molecules.
b. ATP can activate another molecule by transferring a Pi group to the target.
(1) Suppose that an arginine residue in the active site of an enzyme as mutated to
alanine. As expected, the alanine mutant was inactive, suggesting that the
arginine residue was critical to the catalytic mechanism. Which mutation is most
likely to restore wild type level of activity to the alanine mutant?
a. A to M
b. A to S
c. A to K
d. A to Y
e. A to E
c. A to K
(1)
Below is the structure of NADH. List at least 4 functional groups in NADH.
,alcohol, phosphate, ether, alkene, amide
Cetirizine (brand name Zyrtec_ and levocetirizine (brand name Xyzal) are two
histamine H1-receptor antagonists used to treat allergies. Levocetirizine is the
eutomer while cetirizine is the racemic mixture. The enantiomers cannot
interconvert (racemize) in vivo (inside the human body)
a. If the adult dosage for Cetirizine is 10 mg/day, what should the dosage for
levocetirizine be?
5 mg
Cetirizine (brand name Zyrtec_ and levocetirizine (brand name Xyzal) are two
histamine H1-receptor antagonists used to treat allergies. Levocetirizine is the
eutomer while cetirizine is the racemic mixture. The enantiomers cannot
interconvert (racemize) in vivo (inside the human body)
a. What is the main reason for developing drugs in racemic mixtures instead of
pure eutomers?
Cheaper, requires less eutomer and the racemic mixture gives the same result
Cetirizine (brand name Zyrtec_ and levocetirizine (brand name Xyzal) are two
histamine H1-receptor antagonists used to treat allergies. Levocetirizine is the
eutomer while cetirizine is the racemic mixture. The enantiomers cannot
interconvert (racemize) in vivo (inside the human body)
a. What could be the reason why some drugs require one enantiomer and cannot
use a racemic mixture?
Racemic mixture can lead to additional side effects since there are more things present
in the mixture.
, Write foes the full name of the amino acid residues of peptide LKPC, from N
terminus to C terminus.
leucine-lysine-phenyalanine-cysteine
What is the formula of calculating pI?
pI = pKa1 + pKa2/ 2
When you are seeing the order of which proteins elute from a a gel filtration
column, how do you know first to last?
highest molecular weight --> lowest MW
How do you know what protein will stay bound to an anion exchange column
when given pI and pH? Why?
When the pH is higher than the pI, the protein will be deprotenated and thus have a
negative charge. In anion exchange, the negative charge stays bound b/c it uses
positively charged beads.
How do you know if a protein is DNA binding?
It will have the same, or nearly the same pI as pH?
a. If your protein of interest with unknown sequence, what technique could
possibly be used to determine the amino acid sequence of A?
Edman degradation b/c its a small protein, so you could use that intreat of mass
spectrometry.
Below is the sequence of part of an alpha helix:
Lys—Ala—Leu—Glu—Met—Iso—His—Ala
How many full turns are in this helix?
2
COMPLETE SOLUTIONS VERIFIED LATEST UPDATE
(1) Which of the following is true?
a. For a reaction with a negative delta G Keq is less than 1.
b. ATP can activate another molecule by transferring a Pi group to the target.
c. Enzymes increase the rate of reactions through lowering delta G0.
d. The driving force of the hydrophobic effect is attraction between hydrophobic
molecules.
b. ATP can activate another molecule by transferring a Pi group to the target.
(1) Suppose that an arginine residue in the active site of an enzyme as mutated to
alanine. As expected, the alanine mutant was inactive, suggesting that the
arginine residue was critical to the catalytic mechanism. Which mutation is most
likely to restore wild type level of activity to the alanine mutant?
a. A to M
b. A to S
c. A to K
d. A to Y
e. A to E
c. A to K
(1)
Below is the structure of NADH. List at least 4 functional groups in NADH.
,alcohol, phosphate, ether, alkene, amide
Cetirizine (brand name Zyrtec_ and levocetirizine (brand name Xyzal) are two
histamine H1-receptor antagonists used to treat allergies. Levocetirizine is the
eutomer while cetirizine is the racemic mixture. The enantiomers cannot
interconvert (racemize) in vivo (inside the human body)
a. If the adult dosage for Cetirizine is 10 mg/day, what should the dosage for
levocetirizine be?
5 mg
Cetirizine (brand name Zyrtec_ and levocetirizine (brand name Xyzal) are two
histamine H1-receptor antagonists used to treat allergies. Levocetirizine is the
eutomer while cetirizine is the racemic mixture. The enantiomers cannot
interconvert (racemize) in vivo (inside the human body)
a. What is the main reason for developing drugs in racemic mixtures instead of
pure eutomers?
Cheaper, requires less eutomer and the racemic mixture gives the same result
Cetirizine (brand name Zyrtec_ and levocetirizine (brand name Xyzal) are two
histamine H1-receptor antagonists used to treat allergies. Levocetirizine is the
eutomer while cetirizine is the racemic mixture. The enantiomers cannot
interconvert (racemize) in vivo (inside the human body)
a. What could be the reason why some drugs require one enantiomer and cannot
use a racemic mixture?
Racemic mixture can lead to additional side effects since there are more things present
in the mixture.
, Write foes the full name of the amino acid residues of peptide LKPC, from N
terminus to C terminus.
leucine-lysine-phenyalanine-cysteine
What is the formula of calculating pI?
pI = pKa1 + pKa2/ 2
When you are seeing the order of which proteins elute from a a gel filtration
column, how do you know first to last?
highest molecular weight --> lowest MW
How do you know what protein will stay bound to an anion exchange column
when given pI and pH? Why?
When the pH is higher than the pI, the protein will be deprotenated and thus have a
negative charge. In anion exchange, the negative charge stays bound b/c it uses
positively charged beads.
How do you know if a protein is DNA binding?
It will have the same, or nearly the same pI as pH?
a. If your protein of interest with unknown sequence, what technique could
possibly be used to determine the amino acid sequence of A?
Edman degradation b/c its a small protein, so you could use that intreat of mass
spectrometry.
Below is the sequence of part of an alpha helix:
Lys—Ala—Leu—Glu—Met—Iso—His—Ala
How many full turns are in this helix?
2
