BCH 451 FINAL EXAM (EXTRA STUFF) QUESTIONS AND ANSWERS WITH VERIFIED SOLUTIONS

BCH 451 FINAL EXAM (EXTRA STUFF) QUESTIONS AND

ANSWERS WITH VERIFIED SOLUTIONS

Isoelectric Point

pH at which molecule has a charge of 0

Van Der Waals Potential Energy Function

deltaG = (-rij^-6 +rij^-12)/D

Hydrogen Bonding 10,12 Potential Energy Function

deltaG = (-rij^-10 + rij^-12)/D

Ketamine

single bond tautomer of peptide bond

Enamine

double bond tautomer of peptide bond

Positive Cooperativity

Hb oxygen bonding when Ka2 > Ka1

Little Cooperativity

Hb oxygen bonding when Ka3 ~ Ka4

Negative Cooperativity

Hb oxygen bonding when Ka4 < Ka3

amphipathic

Molecule having both phobic and philic character

Chaotrope (like chaos)

substance that promotes protein unfolding

, Kosmotrope

substance that promotes protein folding

Urea, guanidinium

Chaotrope examples

Ammonium Sulfate

Kosmotrope example

3.6, 13

alpha helix has _____ amino acids per turn and ______ atoms per turn.

unfolding

-deltaG unfolding indicates protein ________ (folding/unfolding) is spontaneous

folding

+deltaG unfolding indicates protein ________ (folding/unfolding) is spontaneous

cis-proline

U-turn secondary structure formed by ________.

Chaperones

Protein complexes that aid in protein folding by having a chaotrope rich environment.

B-mercaptoethanol

HS-CH2-CH2-OH. Used to break disulfide bonds

Dithiothreitol

a more efficient reducing agent than BME

Turnover Time

1/Kcat. time for 1 reaction to occur

Oxidoreductase