BIOL 2070 FINAL EXAM QUESTIONS AND ANSWERS WITH
COMPLETE SOLUTIONS VERIFIED
describe 3 stages of PCR
denaturation = separates DNA strands by breaking H bonds
annealing = attaching RNA primers to target DNA sequence
extension = adding dNTPs to extend sequence
in which direction are primers added during PCR?
5' --> 3'
in which direction do the forward and reverse primers bind and get extended?
forward primer = binds at start of sequence (left) and goes in 5' --> 3' direction
reverse primer = binds at end of sequence (right) and ALSO goes in 5' --> 3' direction
both primers are extended in the 5' --> 3' direction, but the reverse primer starts at the
OPPOSITE end of the target sequence compared to the forward primer
forward primer sequence
identical to coding strand
reverse primer sequence
reverse complement to coding strand
during PCR, are primers made into part of the amplified product?
yes, they are made into part of the amplicon
COMPLETE SOLUTIONS VERIFIED
describe 3 stages of PCR
denaturation = separates DNA strands by breaking H bonds
annealing = attaching RNA primers to target DNA sequence
extension = adding dNTPs to extend sequence
in which direction are primers added during PCR?
5' --> 3'
in which direction do the forward and reverse primers bind and get extended?
forward primer = binds at start of sequence (left) and goes in 5' --> 3' direction
reverse primer = binds at end of sequence (right) and ALSO goes in 5' --> 3' direction
both primers are extended in the 5' --> 3' direction, but the reverse primer starts at the
OPPOSITE end of the target sequence compared to the forward primer
forward primer sequence
identical to coding strand
reverse primer sequence
reverse complement to coding strand
during PCR, are primers made into part of the amplified product?
yes, they are made into part of the amplicon
