GRADED
in semen processing, what should the warming device in lab be set at ?
100 degrees
what does the CASA System help do ?
-allows accurate assessment of:
motality
concentration
morphology
DNA fragmentation
vitality
-gives higher accuracy which is most important for freezing
what are 3 things to consider when semen handling
-avoid physical trauma, exposure to light
-measure volume of filtered semen
-determine motality with microscope
give 4 things you should consider when collecting semen
-avoid excessive stallion teasing
-use minimal sterile lube in AV to avoid contamination
-collect every other day to obtain optimum semen quality
-collect stallion at least 2 times before actually needing semen, "clean out the plumping"
what is the ideal semen collection
-high concentration of sperm cells
-low amounts of seminal plasma
-high percentage of progressively motile sperm cells
-low percentage of morphological abnormalities
what is important in progressive motility
the total number of progressively motile and morphologically normal sperm
what should the insemination dose be in progressive motility
100-500 million PMMNS
what is the most common semen extender
the kenney extender
most stallions do not produce sperm until how many months of age?
24 or older
what does the hypo
it releases GnRH which travels to the pituitary
what does the anterior pituitary produce
gonadotropins; which enter the blood circulation and affect cells in the testes
FSH:
LH:
FSH: affects the Sertoli cells
LH: affects the Leydig cells
what are Sertoli cells and what do they do