WGU Biochemistry OA Exam 2025 Questions and
Answers 100% Pass
Bohr Effect chart (low pH) - ✔✔C- High CO2
H- High H+
A- Acidic
R-Release of O2(rt. Shift)
T- tense state (in tissues)
Primary structure - ✔✔Sequence of amino acids that make up a protein
Secondary Structure - ✔✔Sequence of amino acids fold.
Tertiary Structure - ✔✔-more folding in a 3D shape.
-R groups (side chains)
Quaternary Structure - ✔✔Protein has more than 1 polypeptide chain.
Chemical reaction - ✔✔-absorb and release energy
-activation energy
-energy is released (heat & light) when bonds are broken.
Exothermic reaction - ✔✔Exo=to release
Thermo=heat
-chemical reaction that releases more energy than it absorbs (as heat & light)
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,Example of exothermic reaction - ✔✔Cellular respiration- breaks down glucose to make cellular energy
Endothermic reaction - ✔✔Endo=absorb
Thermos=heat
-absorbs more energy than it releases
Example of endothermic reaction - ✔✔Photosynthesis- takes in light to break down to create glucose
Catalyst - ✔✔Substance that will decrease the activation energy needed to start a chemical reaction
Enzymes - ✔✔-catalysts used by cells
-increase the rate of chemical reaction
-specific to substrates
Enzyme structure - ✔✔-they are proteins
-twist, fold, bend to final shape
-shape attracts specific molecules
Substrate - ✔✔Molecules that bind to the enzyme
Active site - ✔✔-location on enzyme where substrate binds
Function of DNA polymerase in process of PCR. - ✔✔-increase expression
-2 genes
-SIRT1 & SIRT2
Function of a molecule - ✔✔Structural component of the lipid bilayer
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, How would lipid production in a cell change in order to maintain fluidity of its cell membrane as it
adapts to lower temperatures? - ✔✔Produce lipids with shorter fatty acid chains
Function of cholesterol - ✔✔Maintains membrane fluidity
Lipid (fat) soluble vitamins - ✔✔A, D, E, K
What stimulates beta-oxidation of fatty acids? - ✔✔An increase in NAD+ concentration in the
mitochondrial matrix
What occurs in an otherwise healthy person whose diet has very few carbs and high levels of fats? -
✔✔Acetone is produced in the blood
Amino acids - ✔✔-Non-polar (hydrophobic)
-Polar charged
-Polar uncharged
Which type of mammalian DNA damage repair requires the presence of the chromosome that is
homologous to the damaged chromosome? - ✔✔Recombination
Xeroxerma Pigmentosum (XP) - ✔✔A recessive genetic disease that occurs when one or more of the genes
that perform nucleotide excision repair are nonfunctional
Why do XP patients have a much higher incidence of skin cancer than the general population? - ✔✔The
mutation of all other genes is higher due to failure to repair.
Assuming 100% reaction efficiency, how many DNA copies are created after the completion of 4 complete
PCR cycles? - ✔✔16
What is the function of DNA polymerase in the process of PCR? - ✔✔It recognizes the primers and uses
the available dNTPs to replicate the template DNA sequence.
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Answers 100% Pass
Bohr Effect chart (low pH) - ✔✔C- High CO2
H- High H+
A- Acidic
R-Release of O2(rt. Shift)
T- tense state (in tissues)
Primary structure - ✔✔Sequence of amino acids that make up a protein
Secondary Structure - ✔✔Sequence of amino acids fold.
Tertiary Structure - ✔✔-more folding in a 3D shape.
-R groups (side chains)
Quaternary Structure - ✔✔Protein has more than 1 polypeptide chain.
Chemical reaction - ✔✔-absorb and release energy
-activation energy
-energy is released (heat & light) when bonds are broken.
Exothermic reaction - ✔✔Exo=to release
Thermo=heat
-chemical reaction that releases more energy than it absorbs (as heat & light)
Page 1/19
Crafted for Academic Insight by KatelynWhitman. All rights reserved © 2025
,Example of exothermic reaction - ✔✔Cellular respiration- breaks down glucose to make cellular energy
Endothermic reaction - ✔✔Endo=absorb
Thermos=heat
-absorbs more energy than it releases
Example of endothermic reaction - ✔✔Photosynthesis- takes in light to break down to create glucose
Catalyst - ✔✔Substance that will decrease the activation energy needed to start a chemical reaction
Enzymes - ✔✔-catalysts used by cells
-increase the rate of chemical reaction
-specific to substrates
Enzyme structure - ✔✔-they are proteins
-twist, fold, bend to final shape
-shape attracts specific molecules
Substrate - ✔✔Molecules that bind to the enzyme
Active site - ✔✔-location on enzyme where substrate binds
Function of DNA polymerase in process of PCR. - ✔✔-increase expression
-2 genes
-SIRT1 & SIRT2
Function of a molecule - ✔✔Structural component of the lipid bilayer
Page 2/19
Crafted for Academic Insight by KatelynWhitman. All rights reserved © 2025
, How would lipid production in a cell change in order to maintain fluidity of its cell membrane as it
adapts to lower temperatures? - ✔✔Produce lipids with shorter fatty acid chains
Function of cholesterol - ✔✔Maintains membrane fluidity
Lipid (fat) soluble vitamins - ✔✔A, D, E, K
What stimulates beta-oxidation of fatty acids? - ✔✔An increase in NAD+ concentration in the
mitochondrial matrix
What occurs in an otherwise healthy person whose diet has very few carbs and high levels of fats? -
✔✔Acetone is produced in the blood
Amino acids - ✔✔-Non-polar (hydrophobic)
-Polar charged
-Polar uncharged
Which type of mammalian DNA damage repair requires the presence of the chromosome that is
homologous to the damaged chromosome? - ✔✔Recombination
Xeroxerma Pigmentosum (XP) - ✔✔A recessive genetic disease that occurs when one or more of the genes
that perform nucleotide excision repair are nonfunctional
Why do XP patients have a much higher incidence of skin cancer than the general population? - ✔✔The
mutation of all other genes is higher due to failure to repair.
Assuming 100% reaction efficiency, how many DNA copies are created after the completion of 4 complete
PCR cycles? - ✔✔16
What is the function of DNA polymerase in the process of PCR? - ✔✔It recognizes the primers and uses
the available dNTPs to replicate the template DNA sequence.
Page 3/19
Crafted for Academic Insight by KatelynWhitman. All rights reserved © 2025
