BI-255 LAB PRACTICAL EXAM QUESTIONS AND
ANSWERS LATEST UPDATE
Micropipette
An instrument used to measure and transfer fluid volumes that are less than 1 mL.
Microliter (μL)
What is the unit of measure used by the micropipette?
Rack
Micropipettes are expensive and delicate instruments. Always replace the pipette back
in its ____.
Slam
Do not ____ the pipette into the tip.
Fluid
Do not let ____ from a pipette tip enter the pipette itself.
Close
Always ____ a pipette tip box after each time you get a tip.
0.001 L
1 mL = ______ L
0.000001 L
1 μL = ______ L
0.001 mL
1 μL = ______ mL
1L
1,000 mL = ______ L
1L
1,000,000 μL = ______ L
1 mL
1,000 μL = ______ mL
Push Button
Parts of Micropipette: Aspirates and dispenses fluid.
Tip Ejector Button
Parts of Micropipette: Ejects disposable tip.
Thumbwheel
Parts of Micropipette: Sets the volume.
Volumeter
Parts of Micropipette: Displays the volume set by the thumbwheel.
Tip
Parts of Micropipette: Place where disposable tip is placed to draw up liquid.
Volumeter
What on the micropipette appears as a window with numbers on the front of the pipette?
It has three digits.
Push Button
The model of the micropipette is found on the tip of the ____ ____.
Ranges
, Different models of micropipettes have different ____ of volumes that they can
accurately measure.
0.2 - 2 μL
What is the range for the P2 micropipette?
2 - 20 μL
What is the range for the P20 micropipette?
20 - 200 μL
What is the range for the P200 micropipette?
200 - 1000 μL
What is the range for the P1000 micropipette?
Thumbwheel
The ____ of the micropipette is black with ridges. Never turn the push button at the top
of the micropipette. You can damage the device.
After the decimal point.
P20: Where is the red digit?
0.02 microliters
P20: What does each tick mark below the bottom digit represent?
No red digit; all digits precede the decimal point.
P200: Where is the red digit?
0.2 microliters
P200: What does each tick mark below the bottom digit represent?
At the top (the top digit is in red).
P1000: Where is the red digit?
2 microliters
P1000: What does each tick mark below the bottom digit represent?
Correct
Use the ____ pipette. Never adjust the volume beyond the maximum setting, but also
do not use pipettes that are too large. Accuracy and precision drop rapidly towards the
lower limit of settings.
Thumbwheel
Rotate the ____ to the desired volume. The push-button can also be used to set the
volume, and it is easier to set the volume this way when wearing gloves. The
thumbwheel can be used when trying to reach the exact final volume needed. Be sure
not to exceed the upper or lower limits of the micropipette.
Disposable Tips
Open the box of ____ ____. Gently but firmly push the tip holder of the micropipette into
the open end of the appropriate size tip. Do not slam the pipette onto the tip. This will
damage the instrument. Firmly seat the proper-sized tip on the end of the pipette.
Close
Always ____ the tip box after you get a tip. This will help prevent contamination of the
tips.
Rack
Pick up the tube with the fluid to be aspirated by the micropipette. Hold the tube by the
body; do not pipet with the tube in ____ or when someone else is holding the tube.
Depress
ANSWERS LATEST UPDATE
Micropipette
An instrument used to measure and transfer fluid volumes that are less than 1 mL.
Microliter (μL)
What is the unit of measure used by the micropipette?
Rack
Micropipettes are expensive and delicate instruments. Always replace the pipette back
in its ____.
Slam
Do not ____ the pipette into the tip.
Fluid
Do not let ____ from a pipette tip enter the pipette itself.
Close
Always ____ a pipette tip box after each time you get a tip.
0.001 L
1 mL = ______ L
0.000001 L
1 μL = ______ L
0.001 mL
1 μL = ______ mL
1L
1,000 mL = ______ L
1L
1,000,000 μL = ______ L
1 mL
1,000 μL = ______ mL
Push Button
Parts of Micropipette: Aspirates and dispenses fluid.
Tip Ejector Button
Parts of Micropipette: Ejects disposable tip.
Thumbwheel
Parts of Micropipette: Sets the volume.
Volumeter
Parts of Micropipette: Displays the volume set by the thumbwheel.
Tip
Parts of Micropipette: Place where disposable tip is placed to draw up liquid.
Volumeter
What on the micropipette appears as a window with numbers on the front of the pipette?
It has three digits.
Push Button
The model of the micropipette is found on the tip of the ____ ____.
Ranges
, Different models of micropipettes have different ____ of volumes that they can
accurately measure.
0.2 - 2 μL
What is the range for the P2 micropipette?
2 - 20 μL
What is the range for the P20 micropipette?
20 - 200 μL
What is the range for the P200 micropipette?
200 - 1000 μL
What is the range for the P1000 micropipette?
Thumbwheel
The ____ of the micropipette is black with ridges. Never turn the push button at the top
of the micropipette. You can damage the device.
After the decimal point.
P20: Where is the red digit?
0.02 microliters
P20: What does each tick mark below the bottom digit represent?
No red digit; all digits precede the decimal point.
P200: Where is the red digit?
0.2 microliters
P200: What does each tick mark below the bottom digit represent?
At the top (the top digit is in red).
P1000: Where is the red digit?
2 microliters
P1000: What does each tick mark below the bottom digit represent?
Correct
Use the ____ pipette. Never adjust the volume beyond the maximum setting, but also
do not use pipettes that are too large. Accuracy and precision drop rapidly towards the
lower limit of settings.
Thumbwheel
Rotate the ____ to the desired volume. The push-button can also be used to set the
volume, and it is easier to set the volume this way when wearing gloves. The
thumbwheel can be used when trying to reach the exact final volume needed. Be sure
not to exceed the upper or lower limits of the micropipette.
Disposable Tips
Open the box of ____ ____. Gently but firmly push the tip holder of the micropipette into
the open end of the appropriate size tip. Do not slam the pipette onto the tip. This will
damage the instrument. Firmly seat the proper-sized tip on the end of the pipette.
Close
Always ____ the tip box after you get a tip. This will help prevent contamination of the
tips.
Rack
Pick up the tube with the fluid to be aspirated by the micropipette. Hold the tube by the
body; do not pipet with the tube in ____ or when someone else is holding the tube.
Depress
