Advanced Biochem ACS Exam
Questions and Answers 100% Solved
Coenzymes required by the pyruvate dehydrogenase multienzyme complex
are - ✔✔Thiamine pyrophosphate, lipoic acid, Fad, CoA, & NAD+
A kinase is an enzyme that... - ✔✔transfers a phosphoryl group from ATP
Which of these contains a thioester bond: - ✔✔succinyl CoA
In setting up an assay for measuring the total amount of an enzyme
present in an unknown sample, the substrate concentration should
preferably be: - ✔✔10 fold higher than Km
Dialysis would be most useful for the separation of: - ✔✔A ammonium
sulfate from an enzyme
An inhibitor that increases Km and does not alter the V max value is - ✔✔A
competitive inhibitor
Which amino acid side chain would not be expected would not be expected
to participate in covalent enzyme catalysis - ✔✔Leu
Which statement is correct concerning the T(deoxy) and R(oxy) states of
tetrameric hemoglobin - ✔✔In the T to R transition, the central cavity of the
1
©JOSHCLAY 2024/2025. YEAR PUBLISHED 2024.
, tetramer decreases in size, preventing the binding of the allosteric
modulator 2,3,-biophosphylglycerate
When the energy needs of the cell are satisfied, acetyl-CoA accumulates
and serves as a positive allosteric effector for - ✔✔pyruvate carboxylase
To determine the Km value for an enzyme reaction, which parameter is
normally varied, while keeping the others constant - ✔✔substrate
concentration
In a yeast cell growing aerobically on glucose, which would provide most of
the energy for growth? - ✔✔glycolysis to TCA cycle and oxidative
phosphorylation
Cleavage of disulfide bonds in globular proteins may be brought about by -
✔✔Reaction with excess B-mercaptoethanol at pH 8 in 6 M urea
The alpha helical secondary structure found in protein - ✔✔is disrupted by
proline, amine H and carbonyl O
What can you conclude about the thermodynamic spontaneity of a reaction
that has a negative value for delta H and positive for delta S - ✔✔The
reaction is spontaneous
2
©JOSHCLAY 2024/2025. YEAR PUBLISHED 2024.
Questions and Answers 100% Solved
Coenzymes required by the pyruvate dehydrogenase multienzyme complex
are - ✔✔Thiamine pyrophosphate, lipoic acid, Fad, CoA, & NAD+
A kinase is an enzyme that... - ✔✔transfers a phosphoryl group from ATP
Which of these contains a thioester bond: - ✔✔succinyl CoA
In setting up an assay for measuring the total amount of an enzyme
present in an unknown sample, the substrate concentration should
preferably be: - ✔✔10 fold higher than Km
Dialysis would be most useful for the separation of: - ✔✔A ammonium
sulfate from an enzyme
An inhibitor that increases Km and does not alter the V max value is - ✔✔A
competitive inhibitor
Which amino acid side chain would not be expected would not be expected
to participate in covalent enzyme catalysis - ✔✔Leu
Which statement is correct concerning the T(deoxy) and R(oxy) states of
tetrameric hemoglobin - ✔✔In the T to R transition, the central cavity of the
1
©JOSHCLAY 2024/2025. YEAR PUBLISHED 2024.
, tetramer decreases in size, preventing the binding of the allosteric
modulator 2,3,-biophosphylglycerate
When the energy needs of the cell are satisfied, acetyl-CoA accumulates
and serves as a positive allosteric effector for - ✔✔pyruvate carboxylase
To determine the Km value for an enzyme reaction, which parameter is
normally varied, while keeping the others constant - ✔✔substrate
concentration
In a yeast cell growing aerobically on glucose, which would provide most of
the energy for growth? - ✔✔glycolysis to TCA cycle and oxidative
phosphorylation
Cleavage of disulfide bonds in globular proteins may be brought about by -
✔✔Reaction with excess B-mercaptoethanol at pH 8 in 6 M urea
The alpha helical secondary structure found in protein - ✔✔is disrupted by
proline, amine H and carbonyl O
What can you conclude about the thermodynamic spontaneity of a reaction
that has a negative value for delta H and positive for delta S - ✔✔The
reaction is spontaneous
2
©JOSHCLAY 2024/2025. YEAR PUBLISHED 2024.
