MICROBIOLOGY PHYSIOLOGICAL TEST
bio-oxidation
study if the organism can digest sugars aerobically or fermentatively
methyl red
indicate whether a glucose fermenter can digest glucose to a mixed organic acid
voges prauskauer
indicate whether a glucose fermenter can digest glucose to a basic end product(acetyl
methylcarbinol)
catalase
test for catalase which digest hydrogen peroxide to water and oxygen
nitrate
test for the presence of the enzyme nitratase which digest nitrates (NO3) to nitrates
(NO2)
starch hydrolisis
test for enzyme amylase which digest starch to glucose
indole
test for enzyme tryptophanase which digest tryptphane to indole
urea hydrolysis
test for enzyme urease which digest urea to alkaline end products
gelatine
test for the enzyme gelatinase which digest gelatin(protein) to amino acids
citrate test
test for enzyme citratase which digest citrate as sole source of carbon to alkaline end
products
H2S production
test for the enzyme desulferase which removes sulfur from sulfur containing amino
acids to form H2S
litmus milk test
test whether the organism can ferment sugar or digest proteins ect.
by fermenting bacteria fermenting lactose sugar to acid end products
what causes acid?
base is formed due to proteolytic bacteria
How is base formed?
white color in meduim due to actively reproducing bacteria
what is litmus reduction?
red to brown translucent(see through) color in medium causes by proteolytic
bacteria
what is peptonization?
to identify the bacterium related to a specific genus and species name for clinical
identification. identify the organism infecting the patient.
what is the main purpose for this unknown experiment?
bio-oxidation
study if the organism can digest sugars aerobically or fermentatively
methyl red
indicate whether a glucose fermenter can digest glucose to a mixed organic acid
voges prauskauer
indicate whether a glucose fermenter can digest glucose to a basic end product(acetyl
methylcarbinol)
catalase
test for catalase which digest hydrogen peroxide to water and oxygen
nitrate
test for the presence of the enzyme nitratase which digest nitrates (NO3) to nitrates
(NO2)
starch hydrolisis
test for enzyme amylase which digest starch to glucose
indole
test for enzyme tryptophanase which digest tryptphane to indole
urea hydrolysis
test for enzyme urease which digest urea to alkaline end products
gelatine
test for the enzyme gelatinase which digest gelatin(protein) to amino acids
citrate test
test for enzyme citratase which digest citrate as sole source of carbon to alkaline end
products
H2S production
test for the enzyme desulferase which removes sulfur from sulfur containing amino
acids to form H2S
litmus milk test
test whether the organism can ferment sugar or digest proteins ect.
by fermenting bacteria fermenting lactose sugar to acid end products
what causes acid?
base is formed due to proteolytic bacteria
How is base formed?
white color in meduim due to actively reproducing bacteria
what is litmus reduction?
red to brown translucent(see through) color in medium causes by proteolytic
bacteria
what is peptonization?
to identify the bacterium related to a specific genus and species name for clinical
identification. identify the organism infecting the patient.
what is the main purpose for this unknown experiment?
