WITH COMPLETE SOLUTIONS VERIFIED GRADED A++
if n of a specimen ~ = n of mounting medium, the image will be
transparent
darkfield microscopy helps brighten the specimen against a
dark background
for brightfield, the intensity of a sample is the result of interference between
deviated and undeviated light through the sample
for darkfield, the undeviated rays from the condenser are
blocked, creates cone of light
for darkfield, only the light rays striking the specimen will be ___ into the
objective
scattered
for darkfield, what diaphragms are opened all the way
aperture and field
for darkfield, the light rays are
refracted, reflected, and scattered
a thin specimen is desirable for darkfield because the features above and below
the focus plane can
scatter light and degrade the image
what kind of intensity is important for darkfield
, high
what kind of accessory is added to the condenser for darkfield
spider stop
two types of darkfield condensers are
paraboloid and cardioid
paraboloid and cardioid darkfield condensers are designed for
high magnification work
paraboloid and cardioid darkfield condensers have no
chromatic aberration
reflected light microscopy is used for
fluorescence, opaque samples, and some darkfield applications
reflected light microscopy is also known as
incident light or epi-illumination
darkfield objective lenses can be abbreviated as
neo, BF/DF, BD or D
in darkfield objective lenses, the light travels through the
hollow outside
in darkfield objective lenses, the light is formed into a
hollow cone by means of mirrors or prisms at bottom of objective
rheinberg illumination is also known as
optical staining
rheinburg illumination is replacing the opaque stop with
a transparent colored filter