BIOS 2225 FINAL REVIEW EXAM QUESTIONS
& ANSWERS RATED 100% CORRECT!!
BSL 1 Answer - no containment; defined organisms; unlikely to cause disease;
good microbial technique (GMT); no safety equipment; open bench; ex: E. coli
BSL 2 Answer - containment; moderate risk; disease varying severity; GMT and
PPE; open bench and biosafety cabinet for potential aerosols; ex: HIV, flu
BSL 3 Answer - high containment; aerosol transmission; serious/potentially
lethal diseases; GMT and special clothing; biosafety cabinet for all activities; ex:
tuberculosis
BSL 4 Answer - maximum containment; "exotic", high risk agents; life
threatening diseases with no treatment; GMT, PPE and airlock entry, shower
entry and exit; biosafety cabinet for positive pressure suites; ex: ebola
when handling BSL 2 organisms, what PPE should be used? Answer - GMT
(good microbial technique) and PPE (personal protective equipment); lab coat,
gloves, and eye protection
total magnification calculation Answer - ocular lens x objective lens
parfocal Answer - a microscope calibrated this way will maintain the specimen
in focus when the objective lens is rotated
resolution Answer - shortest distance between 2 points on a specimen that can
still be distinguished by the observer or camera system as separate entities
, what are the two ways to increase contrast when using a compound light
microscope? Answer -
how many nm are equal to 1 mm, 0.1 mm? Answer - 1000 nm = 1 mm; 100 nm
= 0.1 mm
what is a wet mount? Answer - drop of water on specimen; keeps organisms
alive
prokaryotic organisms Answer - simple DNA; few organelles; very small; DNA
not protected; nucleoid; anaerobic/aerobic; bacteria and archaea
eukaryotic organisms Answer - complex DNA; lots of organelles; larger; DNA
protected; nucleus; aerobic; animal, plant, and fungi
are all freshwater organisms prokaryotic? Answer - no, very diverse
what is a HAB? Answer - harmful algal bloom; photosynthetic algae
are cyanobacteria prokaryotic? Answer - yes
what is microcystin? Answer - toxins that make water toxic, produced by
cyanobacteria
what can we learn about a microbe when looking at it under the microscope?
Answer - morphology, clustering, approx. size, motile or not
& ANSWERS RATED 100% CORRECT!!
BSL 1 Answer - no containment; defined organisms; unlikely to cause disease;
good microbial technique (GMT); no safety equipment; open bench; ex: E. coli
BSL 2 Answer - containment; moderate risk; disease varying severity; GMT and
PPE; open bench and biosafety cabinet for potential aerosols; ex: HIV, flu
BSL 3 Answer - high containment; aerosol transmission; serious/potentially
lethal diseases; GMT and special clothing; biosafety cabinet for all activities; ex:
tuberculosis
BSL 4 Answer - maximum containment; "exotic", high risk agents; life
threatening diseases with no treatment; GMT, PPE and airlock entry, shower
entry and exit; biosafety cabinet for positive pressure suites; ex: ebola
when handling BSL 2 organisms, what PPE should be used? Answer - GMT
(good microbial technique) and PPE (personal protective equipment); lab coat,
gloves, and eye protection
total magnification calculation Answer - ocular lens x objective lens
parfocal Answer - a microscope calibrated this way will maintain the specimen
in focus when the objective lens is rotated
resolution Answer - shortest distance between 2 points on a specimen that can
still be distinguished by the observer or camera system as separate entities
, what are the two ways to increase contrast when using a compound light
microscope? Answer -
how many nm are equal to 1 mm, 0.1 mm? Answer - 1000 nm = 1 mm; 100 nm
= 0.1 mm
what is a wet mount? Answer - drop of water on specimen; keeps organisms
alive
prokaryotic organisms Answer - simple DNA; few organelles; very small; DNA
not protected; nucleoid; anaerobic/aerobic; bacteria and archaea
eukaryotic organisms Answer - complex DNA; lots of organelles; larger; DNA
protected; nucleus; aerobic; animal, plant, and fungi
are all freshwater organisms prokaryotic? Answer - no, very diverse
what is a HAB? Answer - harmful algal bloom; photosynthetic algae
are cyanobacteria prokaryotic? Answer - yes
what is microcystin? Answer - toxins that make water toxic, produced by
cyanobacteria
what can we learn about a microbe when looking at it under the microscope?
Answer - morphology, clustering, approx. size, motile or not
