ASCP CG CERTIFICATION PRACTICE (ACTUAL
2024-2025) WITH ANSWERS.
All of the cultures in a CO2 incubator failed, what aspects of
the incubator are important to monitor to assess the source of
the problem? - <<<Answers>>>>Temperature,
CO2 level, and
Humidity level
The most important factor affecting the quality of
chromosomes viewed under a phase contrast scope is: -
<<<Answers>>>>Slide drying after the cells have been
dropped
Which cell type would you want to use for long term serial
cytogenetic studies? - <<<Answers>>>>Fibroblast cells
What is not a necessary (must be added) ingredient which is
added to modified medium? - <<<Answers>>>>Antibiotics
What chemical is used for cryopreservation? -
<<<Answers>>>>Dimethyl sulfoxide (DMSO)
What would you do if you see high non-specific background
on the FISH slide just postwashed? -
<<<Answers>>>>Increase postwash temperature
, ASCP CG CERTIFICATION PRACTICE (ACTUAL
2024-2025) WITH ANSWERS.
Which is preferred as a metaphase probe? -
<<<Answers>>>>SKY probe
A MLL FISH gave the following signal pattern 1F 1R 1G.
What is the ISCN of this cell? - <<<Answers>>>>nuc ish
(MLLx2)(5?MLL sep 3?MLLx1)
Fluorescence can be caused by what light source? -
<<<Answers>>>>Mercury vapor lamps
To AVOID empty magnification, the total magnification seen
by the eye should: - <<<Answers>>>>Not exceed 1000X the
N.A. of the objective
What ARE functions of the CCD camera? -
<<<Answers>>>>Capturing light
Controlling the resolution of the image
Determination of matrix size based on the number of photo
cells
How many bands are on chromosome 10 in the 350 to 400
band level range? - <<<Answers>>>>12 to 14
How many bands are on chromosome 10 in the 425 to 475
band level range? - <<<Answers>>>>15 to 21
, ASCP CG CERTIFICATION PRACTICE (ACTUAL
2024-2025) WITH ANSWERS.
How many bands are on chromosome 10 in the 500 to 550
band level range? - <<<Answers>>>>22 to 28
AML M0 type - <<<Answers>>>>AML with minimal
differentiation (no chromosome change associated)
AML M1 type - <<<Answers>>>>AML w/o differentiation
(no associated chrom changes either)
AML M2 type - <<<Answers>>>>AML with differentiation
t(8;21)(q22;q22)
AML M3 type - <<<Answers>>>>Acute Promyelocytic
Leukemia (APL)
t(15;17)(q22;q11-12)
AML M4 type - <<<Answers>>>>Acute Myelomonocytic
Leukemia (AMML)
inv(16)(p13q22)
AML M5 type - <<<Answers>>>>Acute Monocytic
Leukemia
t(9;11)(p22;q23)
, ASCP CG CERTIFICATION PRACTICE (ACTUAL
2024-2025) WITH ANSWERS.
other 11q23 rearrangements
AML M6 type - <<<Answers>>>>Acute Erythroleukemia
del(5q), del(7q), +8
AML M7 type - <<<Answers>>>>Acute Megakaryocytic
leukemia
(no chrom abn associated)
You are analyzing a clinical case and discover a small extra
marker with satellites. What staining technique would be most
useful in determining the chromosomal origin of this marker?
- <<<Answers>>>>NOR
When using a fluorescein dye (yellow-green) labeled probe
during the FISH procedure, which counterstain should be
used? - <<<Answers>>>>Propidium iodide (red)
A bone marrow sample collected in transport medium is
leaking into the sealed plastic bag in which it was transported.
The technologist should_______. - <<<Answers>>>>Clean
up the tube while wearing gloves
A laboratory' quality assurance program includes
_______________. - <<<Answers>>>>Proficiency testing
2024-2025) WITH ANSWERS.
All of the cultures in a CO2 incubator failed, what aspects of
the incubator are important to monitor to assess the source of
the problem? - <<<Answers>>>>Temperature,
CO2 level, and
Humidity level
The most important factor affecting the quality of
chromosomes viewed under a phase contrast scope is: -
<<<Answers>>>>Slide drying after the cells have been
dropped
Which cell type would you want to use for long term serial
cytogenetic studies? - <<<Answers>>>>Fibroblast cells
What is not a necessary (must be added) ingredient which is
added to modified medium? - <<<Answers>>>>Antibiotics
What chemical is used for cryopreservation? -
<<<Answers>>>>Dimethyl sulfoxide (DMSO)
What would you do if you see high non-specific background
on the FISH slide just postwashed? -
<<<Answers>>>>Increase postwash temperature
, ASCP CG CERTIFICATION PRACTICE (ACTUAL
2024-2025) WITH ANSWERS.
Which is preferred as a metaphase probe? -
<<<Answers>>>>SKY probe
A MLL FISH gave the following signal pattern 1F 1R 1G.
What is the ISCN of this cell? - <<<Answers>>>>nuc ish
(MLLx2)(5?MLL sep 3?MLLx1)
Fluorescence can be caused by what light source? -
<<<Answers>>>>Mercury vapor lamps
To AVOID empty magnification, the total magnification seen
by the eye should: - <<<Answers>>>>Not exceed 1000X the
N.A. of the objective
What ARE functions of the CCD camera? -
<<<Answers>>>>Capturing light
Controlling the resolution of the image
Determination of matrix size based on the number of photo
cells
How many bands are on chromosome 10 in the 350 to 400
band level range? - <<<Answers>>>>12 to 14
How many bands are on chromosome 10 in the 425 to 475
band level range? - <<<Answers>>>>15 to 21
, ASCP CG CERTIFICATION PRACTICE (ACTUAL
2024-2025) WITH ANSWERS.
How many bands are on chromosome 10 in the 500 to 550
band level range? - <<<Answers>>>>22 to 28
AML M0 type - <<<Answers>>>>AML with minimal
differentiation (no chromosome change associated)
AML M1 type - <<<Answers>>>>AML w/o differentiation
(no associated chrom changes either)
AML M2 type - <<<Answers>>>>AML with differentiation
t(8;21)(q22;q22)
AML M3 type - <<<Answers>>>>Acute Promyelocytic
Leukemia (APL)
t(15;17)(q22;q11-12)
AML M4 type - <<<Answers>>>>Acute Myelomonocytic
Leukemia (AMML)
inv(16)(p13q22)
AML M5 type - <<<Answers>>>>Acute Monocytic
Leukemia
t(9;11)(p22;q23)
, ASCP CG CERTIFICATION PRACTICE (ACTUAL
2024-2025) WITH ANSWERS.
other 11q23 rearrangements
AML M6 type - <<<Answers>>>>Acute Erythroleukemia
del(5q), del(7q), +8
AML M7 type - <<<Answers>>>>Acute Megakaryocytic
leukemia
(no chrom abn associated)
You are analyzing a clinical case and discover a small extra
marker with satellites. What staining technique would be most
useful in determining the chromosomal origin of this marker?
- <<<Answers>>>>NOR
When using a fluorescein dye (yellow-green) labeled probe
during the FISH procedure, which counterstain should be
used? - <<<Answers>>>>Propidium iodide (red)
A bone marrow sample collected in transport medium is
leaking into the sealed plastic bag in which it was transported.
The technologist should_______. - <<<Answers>>>>Clean
up the tube while wearing gloves
A laboratory' quality assurance program includes
_______________. - <<<Answers>>>>Proficiency testing
