ARULMIGU KALASALINGAM
COLLEGE OF PHARMACY
ANAND NAGAR - KRISHNANKOIL
V – SEMESTER (III B. Pharmacy)
PHARMACOLOGY- II
PRACTICAL LAB MANUAL
, BP 507 P. PHARMACOLOGY-II (Practical)
4Hrs/Week
1. Introduction to in-vitro pharmacology and physiological salt solutions.
2. Effect of drugs on isolated frog heart.
3. Effect of drugs on blood pressure and heart rate of dog.
4. Study of diuretic activity of drugs using rats/mice.
5. DRC of acetylcholine using frog rectus abdominis muscle.
6. Effect of physostigmine and atropine on DRC of acetylcholine using frog rectus
abdominis muscle and rat ileum respectively.
7. Bioassay of histamine using guinea pig ileum by matching method.
8. Bioassay of oxytocin using rat uterine horn by interpolation method.
9.Bioassay of serotonin using rat fundus strip by three point bioassay.
10. Bioassay of acetylcholine using rat ileum/colon by four point bioassay.
11. Determination of PA2 value of prazosin using rat anococcygeus muscle (by
Schilds plot method).
12. Determination of PD2 value using guinea pig ileum.
13. Effect of spasmogens and spasmolytics using rabbit jejunum.
14. Anti-inflammatory activity of drugs using carrageenan induced paw-edema
model.
15. Analgesic activity of drug using central and peripheral methods
,STUDENT NAME :
COURSE :
SUBJECT :
YEAR :
INDEX
Page Staff
S.No Date Name of the experiments no marks signature
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
, PHARMACOLOGY-II
EX NO:1
Introduction to In-vitro pharmacology and physiological salt solutions.
Aim: To study the Introduction about In-Vitro Pharmacology and Physiological salt
solutions.
Introduction:
“In vitro” is a latin word that means “within the glass” Therefore the studies which
are done outside the living organism, inside glass (test tubes or petridishes) are known as In
vitro studies.
It is the experiment or observations done on the tissue outside of the living organism
in a controlled environment ,usually using petridishes and testtubes.
In vitro processes ,conditions are artificial and they are reconstructions of In vivo
environments.Artificial conditions are formed by mixing the necessary components and
reagents under controlled conditions inside a glassware in the laboratory .
In vitro methods are widely used in Pharmaceutical industry to produce large scale
pharamaceuticals using microorganism due to ease of production and economic benefits.
Advantages of in-vitro studies :
In vitro studies permit a species-specific.
simpler, more convenient
more detailed analysis
In vitro studies replacing studies in whole animals.
It is less expensive and provides quicker results.
Definition:
Estimation of potency of an active ingredient in unit quantity of preparation is
detection and measurement of concentration of substance preparation using biological
method is known as bioassay.
Principle of Bioassay:
COLLEGE OF PHARMACY
ANAND NAGAR - KRISHNANKOIL
V – SEMESTER (III B. Pharmacy)
PHARMACOLOGY- II
PRACTICAL LAB MANUAL
, BP 507 P. PHARMACOLOGY-II (Practical)
4Hrs/Week
1. Introduction to in-vitro pharmacology and physiological salt solutions.
2. Effect of drugs on isolated frog heart.
3. Effect of drugs on blood pressure and heart rate of dog.
4. Study of diuretic activity of drugs using rats/mice.
5. DRC of acetylcholine using frog rectus abdominis muscle.
6. Effect of physostigmine and atropine on DRC of acetylcholine using frog rectus
abdominis muscle and rat ileum respectively.
7. Bioassay of histamine using guinea pig ileum by matching method.
8. Bioassay of oxytocin using rat uterine horn by interpolation method.
9.Bioassay of serotonin using rat fundus strip by three point bioassay.
10. Bioassay of acetylcholine using rat ileum/colon by four point bioassay.
11. Determination of PA2 value of prazosin using rat anococcygeus muscle (by
Schilds plot method).
12. Determination of PD2 value using guinea pig ileum.
13. Effect of spasmogens and spasmolytics using rabbit jejunum.
14. Anti-inflammatory activity of drugs using carrageenan induced paw-edema
model.
15. Analgesic activity of drug using central and peripheral methods
,STUDENT NAME :
COURSE :
SUBJECT :
YEAR :
INDEX
Page Staff
S.No Date Name of the experiments no marks signature
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
, PHARMACOLOGY-II
EX NO:1
Introduction to In-vitro pharmacology and physiological salt solutions.
Aim: To study the Introduction about In-Vitro Pharmacology and Physiological salt
solutions.
Introduction:
“In vitro” is a latin word that means “within the glass” Therefore the studies which
are done outside the living organism, inside glass (test tubes or petridishes) are known as In
vitro studies.
It is the experiment or observations done on the tissue outside of the living organism
in a controlled environment ,usually using petridishes and testtubes.
In vitro processes ,conditions are artificial and they are reconstructions of In vivo
environments.Artificial conditions are formed by mixing the necessary components and
reagents under controlled conditions inside a glassware in the laboratory .
In vitro methods are widely used in Pharmaceutical industry to produce large scale
pharamaceuticals using microorganism due to ease of production and economic benefits.
Advantages of in-vitro studies :
In vitro studies permit a species-specific.
simpler, more convenient
more detailed analysis
In vitro studies replacing studies in whole animals.
It is less expensive and provides quicker results.
Definition:
Estimation of potency of an active ingredient in unit quantity of preparation is
detection and measurement of concentration of substance preparation using biological
method is known as bioassay.
Principle of Bioassay:
