PCR Exam Review Questions & Answers
2024/2025
Which control samples should you include in your PCR experimental design? - ANSWERS-Positive control
-Negative control
-No template
List the PCR components/reagenst neccessary for PCR. Which are the same for any PCR? - ANSWERS-
Primers: attach to sites on the DNA strands that are at either end of the segment you want to copy.
-Template DNA
SAME v
-dNTPs: these will be used to build new DNA
-PCR buffer
-Taq polymerase: act like tiny machines that read the DNA code then attach matching nucleotides to
create DNA copies
What molecules are present in a dNTP mis? - ANSWERS-dATP
-dCTP
-dGTP
-dTTP
Which reagents will be added into the MM? - ANSWERS-Taq DNA
-Polymerase
-10x buffer
-dNTPS
-Primers
Which reagent is not added to the MM and is added last to the PCR samples? - ANSWERSTemplate DNA
2024/2025
Which control samples should you include in your PCR experimental design? - ANSWERS-Positive control
-Negative control
-No template
List the PCR components/reagenst neccessary for PCR. Which are the same for any PCR? - ANSWERS-
Primers: attach to sites on the DNA strands that are at either end of the segment you want to copy.
-Template DNA
SAME v
-dNTPs: these will be used to build new DNA
-PCR buffer
-Taq polymerase: act like tiny machines that read the DNA code then attach matching nucleotides to
create DNA copies
What molecules are present in a dNTP mis? - ANSWERS-dATP
-dCTP
-dGTP
-dTTP
Which reagents will be added into the MM? - ANSWERS-Taq DNA
-Polymerase
-10x buffer
-dNTPS
-Primers
Which reagent is not added to the MM and is added last to the PCR samples? - ANSWERSTemplate DNA
