PCR Test study Guide Questions &
Answers 2024/2025
Why would you use PCR for - ANSWERSPCR allows for you to only require a small amount od DNA.
Regions commonly amplified? - ANSWERSnon-coding regions because there are more unique
Steps for PCR - ANSWERSDenaturing (90) heats DNA, breaks hydrogen bonds
Annealing (60) primers bracket target and are added
Extension (72) Taq connect nucleotides to primers.
Five reagents of PCR - ANSWERSTaq Polymerase- connects the nucleotides
Primers- bracket the target, tell taq where to start
Free Nucleotides- building blocks
DNA- Original
Buffer- creates best environment for taq, add mg and pH
Why do we use Taq polymerase? - ANSWERSBecause it can function in high temperatures. comes from
thermus aquaticus
Primer design considerations - ANSWERSdon't want primer to attach to itself or another primer.
What is a STR? What makes it special? - ANSWERSShort Tandem repeats
Special because they occur in non coding regions, which means they don't code for protein.
Why are STR's used in PCR? - ANSWERSWe can use the number of repeats to distinguish between
individuals.
Answers 2024/2025
Why would you use PCR for - ANSWERSPCR allows for you to only require a small amount od DNA.
Regions commonly amplified? - ANSWERSnon-coding regions because there are more unique
Steps for PCR - ANSWERSDenaturing (90) heats DNA, breaks hydrogen bonds
Annealing (60) primers bracket target and are added
Extension (72) Taq connect nucleotides to primers.
Five reagents of PCR - ANSWERSTaq Polymerase- connects the nucleotides
Primers- bracket the target, tell taq where to start
Free Nucleotides- building blocks
DNA- Original
Buffer- creates best environment for taq, add mg and pH
Why do we use Taq polymerase? - ANSWERSBecause it can function in high temperatures. comes from
thermus aquaticus
Primer design considerations - ANSWERSdon't want primer to attach to itself or another primer.
What is a STR? What makes it special? - ANSWERSShort Tandem repeats
Special because they occur in non coding regions, which means they don't code for protein.
Why are STR's used in PCR? - ANSWERSWe can use the number of repeats to distinguish between
individuals.
