©Jason MacConnell 2025 ALL RIGHTS RESERVED.
BOC Immunology Exam Questions With
Correct Answers A+
Antinuclear antibody tests are performed to help diagnose: - Answer✔lupus erythematosus
Sera to be tested for IFA-ANA 6 after drawing is best stored at: - Answer✔5C +/- 2C
Antibodies directed at native DNA are MOST frequently associated with this pattern of
fluorescence in he IFA-ANA test?
a) rim
b) diffuse
c) speckled
d) centromere - Answer✔a) rim
The technologist observes apparent homogenous staining of the nucleus of interphase cells while
performing and IFA-ANA, as well as staining of he chromosomes in mitotic cells. This result is:
- Answer✔expected for anti-DNA antibodies
the result of an anti-nuclear antibody test was a titer of 1:320 with a peripheral pattern. Which of
the following sets of results best correlates with these results?
a) anti-dsDNA titer 1:80 and a high titer of antibodies to Sm
b) antimitochondrial antibody titer 1:160, and antibodies to RNP
c) anti-Sc1-70, and antibodies to single-stranded DNA
d) high titers of anti_SS-A and anti-SS-B - Answer✔a) anti-dsDNA titer 1:80 and a high titer of
antibodies to Sm
Systemic lupus erythematosus patients often have which of the following test results?
a) high titers of DNA antibody
b) decreased serum immunoglobulin levels
, ©Jason MacConnell 2025 ALL RIGHTS RESERVED.
c) high titers of anti-smooth muscle antibodies
d) high titers of antimitochondrial antibody - Answer✔a) high titers of DNA antibody
Which of the following is the most sensitive and appropriate method for the detection of
rheumatoid factor?
a) nephelometry
b) immunofixation electrophoresis
c) immunofluorescence
d) manual latex agglutination - Answer✔a) nephelometry
Rheumatoid factor reacts with: - Answer✔gamma globulin-coated particles
A consistently and repeatedly negative IFA-ANA is: - Answer✔strong evidence against
untreated SLE
Positive rheumatoid is generally associated with: - Answer✔hyperglobulinemia
rheumatoid factors are immunoglobulins with specificity for allotypic determinants located on
the: - Answer✔Fc fragment of IgG
Rheumatoid factor in a patient's serum may cause a false: - Answer✔positive test for the
detection of IgM class antibodies
Rheumatoid factors are defined as: - Answer✔autoantibodies with specificity for the Fc portion f
the immunoglobulin (IgG) molecule
High titers of antimicrosomal antibodies are MOST often found in: - Answer✔thyroid disease
In chronic active hepatitis, high titers of which of the following antibodies are seen?
a) antimitochondrial
b) anti-smooth muscle
c) anti-DNA
d) anti-parietal cell - Answer✔b) anti-smooth muscle
Anti-RNA antibodies are often present in individuals having an anti-nuclear antibody
immunofluorescent pattern that is: - Answer✔nucleolar
Anti-extractable nuclear antigens are MOST likely associated with which of the following anti-
nuclear antibody immunofluorescent patterns?
BOC Immunology Exam Questions With
Correct Answers A+
Antinuclear antibody tests are performed to help diagnose: - Answer✔lupus erythematosus
Sera to be tested for IFA-ANA 6 after drawing is best stored at: - Answer✔5C +/- 2C
Antibodies directed at native DNA are MOST frequently associated with this pattern of
fluorescence in he IFA-ANA test?
a) rim
b) diffuse
c) speckled
d) centromere - Answer✔a) rim
The technologist observes apparent homogenous staining of the nucleus of interphase cells while
performing and IFA-ANA, as well as staining of he chromosomes in mitotic cells. This result is:
- Answer✔expected for anti-DNA antibodies
the result of an anti-nuclear antibody test was a titer of 1:320 with a peripheral pattern. Which of
the following sets of results best correlates with these results?
a) anti-dsDNA titer 1:80 and a high titer of antibodies to Sm
b) antimitochondrial antibody titer 1:160, and antibodies to RNP
c) anti-Sc1-70, and antibodies to single-stranded DNA
d) high titers of anti_SS-A and anti-SS-B - Answer✔a) anti-dsDNA titer 1:80 and a high titer of
antibodies to Sm
Systemic lupus erythematosus patients often have which of the following test results?
a) high titers of DNA antibody
b) decreased serum immunoglobulin levels
, ©Jason MacConnell 2025 ALL RIGHTS RESERVED.
c) high titers of anti-smooth muscle antibodies
d) high titers of antimitochondrial antibody - Answer✔a) high titers of DNA antibody
Which of the following is the most sensitive and appropriate method for the detection of
rheumatoid factor?
a) nephelometry
b) immunofixation electrophoresis
c) immunofluorescence
d) manual latex agglutination - Answer✔a) nephelometry
Rheumatoid factor reacts with: - Answer✔gamma globulin-coated particles
A consistently and repeatedly negative IFA-ANA is: - Answer✔strong evidence against
untreated SLE
Positive rheumatoid is generally associated with: - Answer✔hyperglobulinemia
rheumatoid factors are immunoglobulins with specificity for allotypic determinants located on
the: - Answer✔Fc fragment of IgG
Rheumatoid factor in a patient's serum may cause a false: - Answer✔positive test for the
detection of IgM class antibodies
Rheumatoid factors are defined as: - Answer✔autoantibodies with specificity for the Fc portion f
the immunoglobulin (IgG) molecule
High titers of antimicrosomal antibodies are MOST often found in: - Answer✔thyroid disease
In chronic active hepatitis, high titers of which of the following antibodies are seen?
a) antimitochondrial
b) anti-smooth muscle
c) anti-DNA
d) anti-parietal cell - Answer✔b) anti-smooth muscle
Anti-RNA antibodies are often present in individuals having an anti-nuclear antibody
immunofluorescent pattern that is: - Answer✔nucleolar
Anti-extractable nuclear antigens are MOST likely associated with which of the following anti-
nuclear antibody immunofluorescent patterns?
