MICROBIO 150 LAB EXAM 1 PVCC COMPLETE QUESTIONS AND CORRECT ANSWERS | GRADED AND THE LATEST SERIES

2025-2026 SERIES
MICROBIO 150 LAB EXAM 1 PVCC COMPLETE
QUESTIONS AND CORRECT ANSWERS | GRADED
AND THE LATEST SERIES
When preparing to view a specimen on a compound microscope in the lab, one

should __________ begin with the 4x objective


Ans: Always


Question: Where should used gloves be disposed of in the microbiology lab


Ans: In the bio-hazard waste container


Question: Before putting a dust cover over the microscope and placing it in the

cabinet, which objective lens should be rotated over the stage


Ans: 4x


Question: On the microscope, what concentrates the light on the specimen?


Ans: Condenser lens


Question: On the microscope, what bends light passing through the specimen to

create a real image


Ans: Objective lens


Question: On the microscope, what allows for more or less light to be directed

onto the specimen

, 2025-2026 SERIES
Ans: Iris diaphragm


Question: On the microscope, what gives you clarity of the image


Ans: Resolution


Question: Why is desiccating your inoculating loop important? When should this

be done?


Ans: To ensure it is sterilized, done so no cross contamination happens


Question: During an aseptic transfer, when do you pass the mouth of the test

tube through the flame?


Ans: Before and after dipping loop in


Question: During the broth to slide aseptic transfer, a __________ was used to

obtain a pure culture from a __________.


Ans: Loop, broth


Question: Prior to staining, smears of microorganisms are heat-fixed in order to


Ans: Attach it firmly to the slide


Question: The term that refers to the purposeful addition of microorganisms into

a nutrient medium is