SERIES MICROBIOLOGY LAB EXAM 1 NOTE CARDS COMPLETE QUESTIONS AND CORRECT ANSWERS | GRADED AND THE LATEST SERIES

Ans: 1000x;10x ocular x 100X objective Question: what is the purpose of heat fixing Ans: kill the bacteria, adhere to glass anddenature the proteins of bacteria Question: name oneof the stains or regents used in the gram stain procedure Ans: gram crystal violet, gram iodine, gram sfaranin, 95% ethanol Question: why are aseptic techniques used in the lab? Ans: to ensure that we do not contaminate what we are working with as as to ensue that we do not acciently inoculate ouselves. example of aseptic techniques are flaming the loop/needle sterilizing it, breifly flaming the test tube before and after obtaining the sample. Question: the gram reaction is due to differences in the structure of wat part of the microbe Ans: its cell wall, specifically peptidoglycan, gram positive bacteria have thicker peptidoglycan layer compared to GN bacteria. Question: Name 2 inoculating tools you will be using in this lab SERIES Ans: loop, needle Question: what color is a gram positive microbe Ans: purple Question: waht is the function of the ethanol in the gram stain procedure Ans: decolorize Question: name one of the strains in the acid fast stain Ans: cold kinyoun, carbol fuschsin, methylen blue Question: name one of the stains in the endospore stain Ans: malachite green, safranin Question: what is the purpose of the acid-fast stain Ans: the purpose of the acid-fast stain is to differentiate between the myobactriace family of bacteria from all other bacteria Question: what color does na acid-fast microbe stain with acd-fast stain Ans: red Question: what is the function of agarin the media Ans: agar allows media to remain solid at room temperature SERIES Question: what is the difference between broth and agar based media Ans: broth is liquid media at room temperature, agar media is solid at room temp. Question: what is the funciton of media Ans: media is used to provide nutrients to a microbe as well as a growth medium Question: what is the name of the plate inoculation technique Ans: quadrant steak plate Question: Why must the media we are making today be sterilized prior to us? Ans: the meda must be sterilized to remove any bacterial contaminant that may be introduced when the media is made Question: how do you know yo have dont a successful streak plate? Ans: youve ended up with isolated colonies Question: what do you think will happen if you inoculated an obligate anerobe onto a TSA plate and incubated the incubator we have been using? Ans: the obligate anerobe would not grow because it doesn't have the ability to produce th necessary enzymes to dtoxify toxic oxygen species Question: What is one procedure used to determine oxygen requirement of a micrboe SERIES Ans: thioglycollate medium or anaerobic bag Question: why are we working at differnt temperature in the growth curve excersize? Ans: to demonstrate th differences in bacterial growth rate at different temperatures Question: why are we using the spectrophotometer in todarys class Ans: to determine the optical density of our sample. this number will correlate the amount of bacteria in the sample Question: all microbes require oxygen true or false Ans: false Question: name four stages o growth in a closed system growth curve Ans: lag phase, log phase, stati