2025-2026 SERIES
MICROBIOLOGY LAB PRACTICAL 1 STUDY GUIDE
COMPLETE QUESTIONS AND CORRECT ANSWERS |
GRADED AND THE LATEST SERIES
Illuminator
Ans: Filter for shorter λ
Question: Condenser microscope
Ans: Focuses light into specimen
Question: Diaphragm (iris)
Ans: Controls amount of light to specimen
Question: Objective lenses at nosepiece
Ans: Magnifies image 10, 40, or 100 times
Question: Ocular lens
Ans: magnifies image 10X
Question: Course adjustment
Ans: focuses specimen in a broad way
Question: Fine adjustment
, 2025-2026 SERIES
Ans: fine focuses specimen
Question: Stage
Ans: a platform used to hold the slide containing the specimen
Question: Arm
Ans: serves as a handle for carrying microscope
Question: Mechanical stage adjuster
Ans: moves stage forward and back
Question: Stage clip
Ans: Holds microscope slide in position
Question: Magnification
Ans: increase in apparent size
Question: Resolution
Ans: clarity
Question: Oil has the __________ refractive index as glass
Ans: same
, 2025-2026 SERIES
Question: This is the part of the microscope that should not be
touched under high power.
Ans: the course adjustment knob
Question: How does the light intensity change when a student goes from a low
power objective to a high power objective?
Ans: The field of vision narrowed
Question: Which lens do you use oil with?
Ans: 100X lens
Question: How does immersion oil work to enhance resolution?
Ans: Same refractive index as glass; prevents loss of light to air
Question: What is the maximum resolution of your microscope (in micrometers)
or how far apart do two organisms have to be from one another in order to see
them as 2 different organisms under the scope?
Ans: .2 um with 100X
Question: What is the total magnification of your scope when you use each lens?
Ans: ocular lens magnification times the magnification of the objective lens 10X x
40X, 100X, 10X
MICROBIOLOGY LAB PRACTICAL 1 STUDY GUIDE
COMPLETE QUESTIONS AND CORRECT ANSWERS |
GRADED AND THE LATEST SERIES
Illuminator
Ans: Filter for shorter λ
Question: Condenser microscope
Ans: Focuses light into specimen
Question: Diaphragm (iris)
Ans: Controls amount of light to specimen
Question: Objective lenses at nosepiece
Ans: Magnifies image 10, 40, or 100 times
Question: Ocular lens
Ans: magnifies image 10X
Question: Course adjustment
Ans: focuses specimen in a broad way
Question: Fine adjustment
, 2025-2026 SERIES
Ans: fine focuses specimen
Question: Stage
Ans: a platform used to hold the slide containing the specimen
Question: Arm
Ans: serves as a handle for carrying microscope
Question: Mechanical stage adjuster
Ans: moves stage forward and back
Question: Stage clip
Ans: Holds microscope slide in position
Question: Magnification
Ans: increase in apparent size
Question: Resolution
Ans: clarity
Question: Oil has the __________ refractive index as glass
Ans: same
, 2025-2026 SERIES
Question: This is the part of the microscope that should not be
touched under high power.
Ans: the course adjustment knob
Question: How does the light intensity change when a student goes from a low
power objective to a high power objective?
Ans: The field of vision narrowed
Question: Which lens do you use oil with?
Ans: 100X lens
Question: How does immersion oil work to enhance resolution?
Ans: Same refractive index as glass; prevents loss of light to air
Question: What is the maximum resolution of your microscope (in micrometers)
or how far apart do two organisms have to be from one another in order to see
them as 2 different organisms under the scope?
Ans: .2 um with 100X
Question: What is the total magnification of your scope when you use each lens?
Ans: ocular lens magnification times the magnification of the objective lens 10X x
40X, 100X, 10X
