SERIES MICROBIOLOGY LAB FINAL EXAM COMPLETE QUESTIONS AND CORRECT ANSWERS | GRADED AND THE LATEST SERIES

2025-2026 SERIES
MICROBIOLOGY LAB FINAL EXAM COMPLETE
QUESTIONS AND CORRECT ANSWERS | GRADED
AND THE LATEST SERIES
Give the steps of a PCR


Ans: Heat reaction to 95 C to denature DNA
Cool rxn to 55 C to allow primers to anneal
Heat rxn to 72 C to allow Taq polymerase to bind


Question: What machine is used to carry out a PCR reaction?


Ans: thermocycler


Question: How many cycles does a PCR rxn usually go?


Ans: 30


Question: Name the 9 organisms used in the unknown lab

, 2025-2026 SERIES
Ans: Bacillus subtillis
Enterobacter aerogenes
Entero cloacae
Escherichia coli
Klebsiella pneumoniae
Pseudomonas aeruginosa
Salmonella typhimurium
Staphylococcus aureus
Staphylococcus epidermidis


Question: Which organisms can ferment lactose?


Ans: E. coli
E. cloacae
E. aerogenes
K. pneumonia


Question: Define caliform bacteria


Ans: rod-shaped, Gram-negative, non-spore forming, and motile or non-motile
bacteria which can ferment lactose with the production of acid and gas


Question: Name the selective medias used in class