2025-2026 SERIES
MICROBIOLOGY LAB QUIZ 1 COMPLETE QUESTIONS
AND CORRECT ANSWERS | GRADED AND THE
LATEST SERIES
Proper use of the microscope
Ans: all specimens must be viewed under cover glass, focus with scanning
objectives first, use course adjustment knob, once in focus, use the fine focusing knob
(when viewing with low or high power objectives)
Question:Depth of Field
Ans: refers to the thickness of the plane of focus
Question:Field of View
Ans: area that is visible when using the microscope
Question:Why use a microscope?
Ans: To view and study the microscopic world of organisms and objects less than
5mm in size that cohabitate with us on our globe.
Question:Ocular lens (location and job)
Ans: Area you place your eyes to see in scope (top of the tube) - magnifies the
image formed by the objective lens
Question:Objective lenses (location and job)
Ans: the lenses right above where the sample is placed to be viewed. Can be
changed to different magnifications (scanning, low, high). - magnifies the object
, 2025-2026 SERIES
Question:Specimen holder (location and job)
Ans: located right under the objective lenses - holds specimen in place
Question:Mechanical Stage (location and job)
Ans: platform that you place your slides on - holds them in place with help of
specimen holders and can be be moved up and down or left to right
Question:Light intensity control (location and job)
Ans: usually by or with the main power switch. Controls the intensity of the light
Question:x-y axis control knob (location and job)
Ans: attached to the stage (mechanical) - moves the stage along the x/y axis.
One knob for back/front, one knob for left/right
Question:Course focusing knob (location and job)
Ans: located on side of microscope usually with fine focus knob - rough focus
knob only used with high power objective
Question:Fine focusing knob (location and job)
Ans: located on side of microscope usually with coarse focus knob - focus knob
used to fine tune the focus on the specimen
Question:Iris diaphragm (location and job)
MICROBIOLOGY LAB QUIZ 1 COMPLETE QUESTIONS
AND CORRECT ANSWERS | GRADED AND THE
LATEST SERIES
Proper use of the microscope
Ans: all specimens must be viewed under cover glass, focus with scanning
objectives first, use course adjustment knob, once in focus, use the fine focusing knob
(when viewing with low or high power objectives)
Question:Depth of Field
Ans: refers to the thickness of the plane of focus
Question:Field of View
Ans: area that is visible when using the microscope
Question:Why use a microscope?
Ans: To view and study the microscopic world of organisms and objects less than
5mm in size that cohabitate with us on our globe.
Question:Ocular lens (location and job)
Ans: Area you place your eyes to see in scope (top of the tube) - magnifies the
image formed by the objective lens
Question:Objective lenses (location and job)
Ans: the lenses right above where the sample is placed to be viewed. Can be
changed to different magnifications (scanning, low, high). - magnifies the object
, 2025-2026 SERIES
Question:Specimen holder (location and job)
Ans: located right under the objective lenses - holds specimen in place
Question:Mechanical Stage (location and job)
Ans: platform that you place your slides on - holds them in place with help of
specimen holders and can be be moved up and down or left to right
Question:Light intensity control (location and job)
Ans: usually by or with the main power switch. Controls the intensity of the light
Question:x-y axis control knob (location and job)
Ans: attached to the stage (mechanical) - moves the stage along the x/y axis.
One knob for back/front, one knob for left/right
Question:Course focusing knob (location and job)
Ans: located on side of microscope usually with fine focus knob - rough focus
knob only used with high power objective
Question:Fine focusing knob (location and job)
Ans: located on side of microscope usually with coarse focus knob - focus knob
used to fine tune the focus on the specimen
Question:Iris diaphragm (location and job)
