BLD 324 EXAM 1 QUESTIONS AND ANSWERS WITH COMPLETE
SOLUTIONS GRADED A++
Pronormoblast, basophilic (normoblast), polychromatic (normoblast),
orthochromatic (normoblast), polychromatic erythrocyte, erythrocyte
Normoblast order from immature to mature
Proerythroblast, basophilic, polychromatic, orthochromatic, polychromatic
erythrocyte, erythrocyte
Erythroblast order from immature to mature
rubriblast, prorubricyte, rubricyte, metarubricyte, reticulocyte, erythrocyte
Rubriblast order from immature to mature
Polychromatophilic normoblast
The last erythrocyte precursor that is able to divide (in normoblasts)
True
N:C ratio decreases during normoblastic maturation (T/F)
Pronormoblast (proerythroblast, rubriblast)
First RBC precursor
Pronormoblast, proerythroblast, rubriblast
Has 8:1 NC ratio, fine reticular chromatin, basophilic (blue) cytoplasm with NO granules,
NO hemoglobin formed yet... but iron uptake begins as well as global synthesis, 20-25
microns, TEM used
Basophilic normoblast, erythroblast, prorubricyte
,Has 6:1 NC ratio, coarser chromatin, active iron uptake, hemoglobin formation begins
(not visible yet), nucleoli not visible, 16-20 microns
Polychromatic normoblast, eyrthroblast, rubricyte
Has 2:1 NC ratio, coarse and condensed chromatin, hemoglobin is visible = muddy
cytoplasm appearance, color is variable, 12-15 microns, wright stain used
Orthochromic normoblast, erythroblast, metarubricyte
Has 1:2 NC ratio, extremely condensed chromatin (pyknotic), salmon-ish cytoplasm,
hemoglobin synth continues, nucleus can't divide and will be removed from cell at next
stage, 10-12 microns, wright stain used
Polychromatic erythrocyte, reticulocyte
No nucleus (no NC ratio), 7-10 microns, similar cytoplasmic color close to mature RBC,
still has sticky membrane so it will not be released into the bloodstream until later in this
stage
Erythrocyte
No nucleus, +- 7 microns, salmon pink cytoplasm stain, central pallor
Self renewal, differentiation, apoptosis
Three fates of stem cells
granulocyte colony stimulating factor
G-CSF
megakaryocyte colony stimulating factor
GM-CSF
Interleukins
cytokines produced by leukocytes (WBCs)
, Pluripotent
Stem cells that give rise to all the marrow cells
Hematopoetic (HSC)
Give rise to lymphoid and myeloid
CD34, no HLA-DR or other antigens
HSCs are characterized by
Progenitor
Undifferentiated hematopoietic cells has committed to a cell line but cannot be identified
morphologically
CD33, CD38
Myeloid progenitors
CD10, CD38
Lymphoid progenitors
Myeloid progenitors
CD33, CD38
Lymphoid progenitors
CD10, CD38
CD7
T-lymphoid progenitor and NK cells
T-lymphoid progenitor and NK cells
CD7
CD19
B-lymphoid progenitor
SOLUTIONS GRADED A++
Pronormoblast, basophilic (normoblast), polychromatic (normoblast),
orthochromatic (normoblast), polychromatic erythrocyte, erythrocyte
Normoblast order from immature to mature
Proerythroblast, basophilic, polychromatic, orthochromatic, polychromatic
erythrocyte, erythrocyte
Erythroblast order from immature to mature
rubriblast, prorubricyte, rubricyte, metarubricyte, reticulocyte, erythrocyte
Rubriblast order from immature to mature
Polychromatophilic normoblast
The last erythrocyte precursor that is able to divide (in normoblasts)
True
N:C ratio decreases during normoblastic maturation (T/F)
Pronormoblast (proerythroblast, rubriblast)
First RBC precursor
Pronormoblast, proerythroblast, rubriblast
Has 8:1 NC ratio, fine reticular chromatin, basophilic (blue) cytoplasm with NO granules,
NO hemoglobin formed yet... but iron uptake begins as well as global synthesis, 20-25
microns, TEM used
Basophilic normoblast, erythroblast, prorubricyte
,Has 6:1 NC ratio, coarser chromatin, active iron uptake, hemoglobin formation begins
(not visible yet), nucleoli not visible, 16-20 microns
Polychromatic normoblast, eyrthroblast, rubricyte
Has 2:1 NC ratio, coarse and condensed chromatin, hemoglobin is visible = muddy
cytoplasm appearance, color is variable, 12-15 microns, wright stain used
Orthochromic normoblast, erythroblast, metarubricyte
Has 1:2 NC ratio, extremely condensed chromatin (pyknotic), salmon-ish cytoplasm,
hemoglobin synth continues, nucleus can't divide and will be removed from cell at next
stage, 10-12 microns, wright stain used
Polychromatic erythrocyte, reticulocyte
No nucleus (no NC ratio), 7-10 microns, similar cytoplasmic color close to mature RBC,
still has sticky membrane so it will not be released into the bloodstream until later in this
stage
Erythrocyte
No nucleus, +- 7 microns, salmon pink cytoplasm stain, central pallor
Self renewal, differentiation, apoptosis
Three fates of stem cells
granulocyte colony stimulating factor
G-CSF
megakaryocyte colony stimulating factor
GM-CSF
Interleukins
cytokines produced by leukocytes (WBCs)
, Pluripotent
Stem cells that give rise to all the marrow cells
Hematopoetic (HSC)
Give rise to lymphoid and myeloid
CD34, no HLA-DR or other antigens
HSCs are characterized by
Progenitor
Undifferentiated hematopoietic cells has committed to a cell line but cannot be identified
morphologically
CD33, CD38
Myeloid progenitors
CD10, CD38
Lymphoid progenitors
Myeloid progenitors
CD33, CD38
Lymphoid progenitors
CD10, CD38
CD7
T-lymphoid progenitor and NK cells
T-lymphoid progenitor and NK cells
CD7
CD19
B-lymphoid progenitor
