BLD 324 FINAL EXAM STUDY GUIDE EXAM QUESTIONS
AND ANSWERS WITH COMPLETE SOLUTIONS GRADED
A++ LATEST UPDATE
Name the two erythroid progenitors
BFU-E: burst-forming unit erythroid
CFU-E: colony-forming erythroid
Using three different nomenclatures, list in order the stages of red blood cell
development
Describe three mitotic divisions involved in maturation of normoblasts
pronormoblast
- Has nucleus
- 20-25 microns in diameter
- iron uptake begins
- Round, slightly oval
- hemoglobin synthesis is gearing up
- no hemoglobin formed yet
Describe three mitotic divisions involved in maturation of normoblasts
Basophilic Normoblast
,- 16-20 microns in diameter
- has a nucleus
- active iron uptake
- hemoglobin formation begins
Describe three mitotic divisions involved in maturation of normoblasts
Polychromatic
- 12-15 microns
- has nucleus
- hemoglobin first becomes visible
- active hemoglobin production
Name the earliest precursor that can be identified morphologically
Pronormoblast-round, slightly oval
Describe the general morphological changes during normoblastic maturation
Round, slightly oval
Round, slightly smaller
Round, slightly smaller-blue-gray to gray
Round, smaller-salmon pink, but may still be gray
Round, slightly smaller-salmon pink, but may be bluish
Donut shaped, salmon pink
Describe the morphology, cellular activity (RNA, DNA, Hb synthesis, mitosis(and
anatomical location of each of the states of red blood cell development
,Pronormoblast
RNA-active synthesis
DNA-nucleus intact
Hb Synthesis- none
Mitosis-capable
Basophilic Normoblast
RNA-active synthesis
DNA-nucleus intact
Hb Synthesis-production has begun
Mitosis-capable
Polychromatic Normoblast
RNA-yes
DNA-yes
Hb Synthesis-yes
Mitosis-capable (last stage that can)
Orthochromatic Normoblast
RNA-no
DNA-no
Hb Synthesis-yes
Mitosis-no
, Polychromatic Erythrocyte
RNA-no
DNA-no
Hb Synthesis-completed
Mitosis-n
Describe the effects of erythropoietin, its source, stimulus to production,
chemical nature and mechanism of action
Effects-glycoprotein hormone, major stimulatory cytokine for RBC's,
Stimulus to production-when oxygen carrying capacity of blood decrease
Source-N/A
Action-puts more RBC's into circulation
Explain EPO rescue
CFU-E most sensitive
State the time line for normal RBC development from blast to mature cell
BFU-E to pronormblast-14day
Pronormoblast to mature-4-7 days
Describe the role of apoptosis in erythropoiesis
Homeostasis achieved by apoptosis of precursors and progenitors
If there are enough old cells-restricts production of younger cells
Not enough old cells-does not restrict production of younger cells
Apoptosis receptor by young precursors-Fas
Apoptosis ligand by old precursors-FasL
State the normal life span of a RBC
AND ANSWERS WITH COMPLETE SOLUTIONS GRADED
A++ LATEST UPDATE
Name the two erythroid progenitors
BFU-E: burst-forming unit erythroid
CFU-E: colony-forming erythroid
Using three different nomenclatures, list in order the stages of red blood cell
development
Describe three mitotic divisions involved in maturation of normoblasts
pronormoblast
- Has nucleus
- 20-25 microns in diameter
- iron uptake begins
- Round, slightly oval
- hemoglobin synthesis is gearing up
- no hemoglobin formed yet
Describe three mitotic divisions involved in maturation of normoblasts
Basophilic Normoblast
,- 16-20 microns in diameter
- has a nucleus
- active iron uptake
- hemoglobin formation begins
Describe three mitotic divisions involved in maturation of normoblasts
Polychromatic
- 12-15 microns
- has nucleus
- hemoglobin first becomes visible
- active hemoglobin production
Name the earliest precursor that can be identified morphologically
Pronormoblast-round, slightly oval
Describe the general morphological changes during normoblastic maturation
Round, slightly oval
Round, slightly smaller
Round, slightly smaller-blue-gray to gray
Round, smaller-salmon pink, but may still be gray
Round, slightly smaller-salmon pink, but may be bluish
Donut shaped, salmon pink
Describe the morphology, cellular activity (RNA, DNA, Hb synthesis, mitosis(and
anatomical location of each of the states of red blood cell development
,Pronormoblast
RNA-active synthesis
DNA-nucleus intact
Hb Synthesis- none
Mitosis-capable
Basophilic Normoblast
RNA-active synthesis
DNA-nucleus intact
Hb Synthesis-production has begun
Mitosis-capable
Polychromatic Normoblast
RNA-yes
DNA-yes
Hb Synthesis-yes
Mitosis-capable (last stage that can)
Orthochromatic Normoblast
RNA-no
DNA-no
Hb Synthesis-yes
Mitosis-no
, Polychromatic Erythrocyte
RNA-no
DNA-no
Hb Synthesis-completed
Mitosis-n
Describe the effects of erythropoietin, its source, stimulus to production,
chemical nature and mechanism of action
Effects-glycoprotein hormone, major stimulatory cytokine for RBC's,
Stimulus to production-when oxygen carrying capacity of blood decrease
Source-N/A
Action-puts more RBC's into circulation
Explain EPO rescue
CFU-E most sensitive
State the time line for normal RBC development from blast to mature cell
BFU-E to pronormblast-14day
Pronormoblast to mature-4-7 days
Describe the role of apoptosis in erythropoiesis
Homeostasis achieved by apoptosis of precursors and progenitors
If there are enough old cells-restricts production of younger cells
Not enough old cells-does not restrict production of younger cells
Apoptosis receptor by young precursors-Fas
Apoptosis ligand by old precursors-FasL
State the normal life span of a RBC
