b b b b b b b b
Edition By John W Foster, Zarrintaj Aliabadi, Joan L Slon
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czewski
Whatbisbthebconceptbofbspontaneousbgeneration?b-
bANSWERbbblivingborganismsbcouldbdevelopbfrombnonlivingborbdecomposingbmatter
Whobchallengedbthebconceptbofbspontaneousbgenerationbbybshowingbthatbmaggotsbonb
decayingbmeatbcamebfrombflies,bnotbfrombthebmeatbitself?b-bANSWERbbbFrancescobRedi
Whobshowedbthatbmuttonbbrothbboiledbinbflasksbandbthenbsealedbcouldbstillbdevelopbmicr
oorganisms,bwhichbsupportedbthebtheorybofbspontaneousbgeneration?b-
bANSWERbbbJohnbNeedham
Whobshowedbthatbflasksbsealedbandbthenbboiledbhadbnobgrowthbofbmicroorganisms?b-
bANSWERbbbLazzarobSpallanzani
Whobproposedbthatbairbcarriedbgerms?b-bANSWERbbbSpallanzani
Whobdisprovedbthebtheorybofbspontaneousbgeneration?b-bANSWERbbbLouisbPasteur
Whobdemonstratedbthatbdustbcarriedbmicrobesbandbprovedbevidencebforbthebexistenceb
ofbheat-bresistantbformsbofbbacteria?b-bANSWERbbbJohnbTyndall
Whobshowedbthatbabsilkwormbdiseasebwasbcausedbbybabfungus?b-
bANSWERbbbAgostinobBassi
WhobdemonstratedbthatbthebGreatbPotatobBlightbwasbcausedbbybabwaterbmold?b-
bANSWERbbbM.bJ.bBerkely
Whobshowedbthatbthebpebrinebdiseasebofbsilkwormsbwasbcausedbbybabprotozoanbparasi
te?b-bANSWERbbbLouisbPasteur
Whobdevelopedbabsystembofbsurgerybdesignedbtobpreventbmicroorganismsbfrombenterin
gbwounds?b-bANSWERbbbJohnbLister
Whobtransformedbthebpracticebofbsurgery?b-bANSWERbbbJohnbLister
WhobestablishedbthebrelationshipbbetweenbBacillusbanthracisbandbanthrax?b-
bANSWERbbbRobertbKoch
WhatbarebthebKochbPostulates?b-
bANSWERbbb1.Thebmicroorganismsbmustbbebpresentbinbeverybcasebofbthebdiseasebbutba
bsentbfrombhealthybindividualsb
2.Thebsuspectedbmicroorganismsbmustbbebisolatedbandbgrownbinbpurebcultureb
,3.bThebsamebdiseasebmustbresultbwhenbthebisolatedbmicroorganismbisbinoculatedbintobab
healthybhostb
4.bThebsamebmicroorganismbmustbbebisolatedbagainbfrombthebdiseasedbhost
LimitationsbofbKoch'sbPostulatesb-bANSWERbbb-
Someborganismsbcannotbbebgrownbinbpurebculture
-Usingbhumansbinbcompletingbthebpostulatesbisbunethical
-Molecularbandbgeneticbevidencebmaybreplace
WhatbvaccinationbdidbEdwardbJennerbmake?b-bANSWERbbbsmallpoxbvaccination
WhatbvaccinationsbdidbPasteurbproduce?b-
bANSWERbbbchickenbcholera,brabiesbandbanthrax
Whobinducedbformationbofbdippedbtoxin-antitoxinsbrabbitsb-
bANSWERbbbEmilbvonbBehringbandbShibasaburobKitasato
Whobshowedbthatbthebsilkwormbdiseasebwasbcausedbbybfungus?b-bANSWERbbbBassi
WhobdemonstratedbthatbthebGreatbPotatobBlightbofbIrelandbwasbcausedbbybabwaterbmold
?b-bANSWERbbbM.J.bBerkeley
Whobshowedbthatbthebperbrinebdiseasebofbsilkwormsbwasbcausedbbybabprotozoanbparas
ite?b-bANSWERbbbLouisbPasteur
Absimplebmorphologybandblackbabtruebmembrane-delimitedbnucleusb-
bANSWERbbbprokaryotes
Whatbisbabmorphologicallybcomplexbandbhasbabmembrane-enclosedbnucleus?b-
bANSWERbbbEucaryotes
Whatbletbtobthebdiscoverybofbvirusesbasbdiseasebcausingbagents?b-
bANSWERbbbbacterialbfilterbthatbremovedbbacteriabandblargerbmicrobesb(CharlesbChamb
erland)
Thebfivebkingdombclassificationbschemebisb-
bANSWERbbbthebMoneraborbProcaryotae,bProtista,bFungi,bAnimalia,bandbPlantae
Whatbarebribozymes?b-
bANSWERbbbRNAbmoleculesbthatbformbpeptidebbondsbandbperformbcellularbworkbandbre
plication
Thebthreebdomainbschemebisb-bANSWERbbbBacteria,bArchaea,bandbEucarya
Thebthreebdomainbschemebisbbasedbon?b-
bANSWERbbbabcomparaisonbofbribosomalbRNA
Thebearliestbcellsbmaybhavebbeenb-bANSWERbbbRNAbsurroundedbbybliposomes
Whatbarebthebthreebbacterialbmorphology?b-bANSWERbbb-bacillusb(littlebrod)
,-coccusb(grainborbberry)
-spirillumb(coiledborbhelical)
Whatbarebthebtypesbofblightbmicroscopes?b-bANSWERbbb-bright-fieldbmicroscope
-dark-fieldbmicroscope
-phase-contrastbmicroscope
-fluorescencebmicroscopes
Whatbisbabmeasurebofbhowbgreatlybabsubstancebslowsbthebvelocitybofblight?b-
bANSWERbbbRefractivebindex
Thebdirectionbandbmagnitudebofbbendingblightbinbabmicroscopebisbdeterminedbbybtheb___
__b-bANSWERbbbrefractivebindexesbofbthebtwobmediabformingbthebinterface
Whichbmicroscopebproducesbabdarkbimagebagainstbabbrighterbbackground?b-
bANSWERbbbthebbright-fieldbmicroscope
Whatbisbthebproductbofbthebmagnificationsbofbthebocularblensbandbthebobjectiveblens?b-
bANSWERbbbthebtotalbmagnification
Whatbisbthebtotalbmagnificationbofbthebbrightbfield,bdarkbfield,bandbphase-
contrastbmicroscopes?b-bANSWERbbb2000x
Whatbisbthebtotalbresolutionbofbthebbrightbfield,bdarkbfield,bandbphasebcontrastbmicroscop
es?b-bANSWERbbb0.2um
Whatbisbthebpurposebofbthebbrightbfieldbmicroscope?b-
bANSWERbbbforblivebandbpreservedbstainedbspecimens
Whatbisbthebpurposebofbthebdarkbfieldbmicroscope?b-
bANSWERbbbtobobservebliving,bunstainedbpreparations
Whichbmicroscopesbarebgoodbforbobservingblivingbcells?b-bANSWERbbb-
ThebDifferentialbInterferencebContrastbMicroscope
-Phase-contrastbmicroscope
Whichbmicroscopebenhancesbthebcontrastbbetweenbintracellularbstructuresbhavingbsligh
tbdifferencesbinbthebrefractivebindex?b-bANSWERbbbphase-contrastbmicroscope
Whichbmicroscopebshowsbabbrightbimagebofbthebobjectbresultingbfrombthebfluorescentblig
htbemittedbbybthebspecimen?b-bANSWERbbbthebfluorescencebmicroscope
Thebfluorescencebmicroscopebexposesbthebspecimenbtobwhatbtypesbofblight?b-
bANSWERbbbuv,bviolet,borbblue
Thebdistancebbetweenbthebcenterbofbtheblensbandbfocalbpoint?b-bANSWERbbbfocalblength
Thebshorterbthebfocalbpoint,btheb______bthebmagnificationb-bANSWERbbbgreater
Thebshorterbthebwavelength,btheb______bthebresolutionb-bANSWERbbbgreater
, Thebdistancebbetweenbthebfrontbsurfacebofblensbandbsurfacebofbcoverbglassbonbspecime
nb-bANSWERbbbWorkingbdistance
Asbthebworkingbdistancebdecrease,bthebfieldbofbviewb_____b-bANSWERbbbdecrease
Asbthebfieldbofbviewbdecrease,btheblightbgetsb____b-bANSWERbbbdimmer
Whatbisbthebpurposebofbaddingbthebimmersionboil?b-
bANSWERbbbtobobtainbmoreblightbbybprovidingbthebsamebrefractionbenergybasbglass
Asbthebmagnificationbincreases,bwhatbhappensbtobthebfocalblength?b-
bANSWERbbbitbdecrease
Whatbisbthebchargebofbabbacteriabcellbsurface?b-bANSWERbbbnegativebcharge
Whatbisbthebchargebofbbasicbdyes?b-bANSWERbbbpositivebcharge
Whatbisbthebchargebofbacidbdyes?b-bANSWERbbbnegativebcharge
Abthin,buniformbfilmbofbbacterialbgrowthbonbabglassbslidebinborderbtobproceedbwithbfurtherb
stainingbforbmicroscopicbexaminationb-bANSWERbbbsmear
Thebprocessbbybwhichborganismbisbkilledbandbfirmlybattachedbtobmicroscopebslideb-
bANSWERbbbfixation
Heatbfixationbvs.bChemicalbfixingb(notbthebprocedure)b-bANSWERbbb-
heatbfixationbpreservesboverallbshapebbutbnotbinternalbstructures
-
chemicalbfixingbprotectsbfinebcellularbstructurebandbshapebofblarger,bmorebdelicateborga
nisms
Whatbinformationbdoesbdifferentialbstainingbprovide?b-
bANSWERbbbdividesbmicroorganismsbintobgroupsbbasedbonbtheirbstainingbproperties
Whatbisbthebmostbwidelybusedbdifferentialbstainingbprocedure?b-
bANSWERbbbgrambstaining
Negativebstainingb-bANSWERbbbthebbacteriabisbnotbstained;bthebbackgroundbisbstained
Whatbisbshadowingbinbelectronbmicroscopy?b-
bANSWERbbbcoatingbspecimenbwithbabthinbfilmbof bheavybmetal
WhatbisbthebtotalbmagnificationbofbthebTransmissionbElectronbMicroscopebandbthebScan
ningbElectronbMicroscope?b-bANSWERbbb1,000,000x
WhatbisbthebtotalbmagnificationbofbthebScanningbElectronbMicroscope?b-
bANSWERbbb1,000,000x