BLD 434 TOPIC 5 EXAM QUESTIONS AND ANSWERS WITH COMPLETE SOLUTIONS GRADED A++

BLD 434 TOPIC 5 EXAM QUESTIONS AND ANSWERS WITH COMPLETE SOLUTIONS GRADED A++ Immunoglobin (Ig): AKA antibodies if secreted or BCR if on surface proteins produced by Ig gene B cells indicated by rectangle in text book CDR: complementarity determining regions loops in beta sheets make up Ag binding site allows for function are in the VARIABLE region NOT constant Hypervariable region: AKA CDR's RSS: recombination signal sequences flank each V, D, and J segment direct the RAG enzymes have heptamers, nonamers, and 12/23 nucleotides spacers TdT: terminal deoxynucleotidal transferase adds N nucleotides in junctional diversity RAG: binds and cuts the double stranded DNA in RSS's Combinatorial diversity: created by new novel joints when RAG enzymes cleave RSS's (somatic recombination) Junctional diversity: adds huge portion of Ig diversity caused by addition of P and N nucleotides between segments of VARIABLE domain P nucleotides: short palindromic sequences formed by cleavage of hairpins that form at blunt-end cut sites N nucleotides: