BLD 434 EXAM 2 QUESTIONS AND ANSWERS WITH
COMPLETE SOLUTIONS GRADED A++
pre-BCR
checkpoint in early B cell development that monitors the production of Ig-mu heavy
chain
surrogate light chain
VPreB + lambda5
RAG-1/RAG-2
recognize recombination sequences and make double stranded DNA cuts to initiate
recombination
CAM (cell adhesion molecule)
important signal for developing B cells in bone marrow
polyspecific antibody
have the ability to bind a number of different antigens
B-1 cells have them
cell stages of B cell development
stem cell --> pro-B cell --> pre-B cell --> immature B cell --> naive mature B cell
pro-B cell
earliest identifiable B lineage cell
pre-B cell
,has transient expression of pre-BCR, rearranged Ig heavy chain, begins V-J
rearrangement of light chain
immature B cell
have surface expression of productively rearranged heavy chain plus rearranged light
chain
why is it advantageous for a pre-B cell to undergo a burst of proliferation
following signaling through the pre-BCR?
the burst allows the pre-B cell to express rearranged mu heavy chain with surrogate
light chain
what stage during B cell development are RAG-1 and RAG-2 expressed?
Pro-B and Pre-B cells
the role of stromal cells during B cell development and where they are located in
the body
...
the two growth factors that support B cell development in the bone marrow
stem cell factor (SCF)
soluble cytokine growth factor IL-7
what fraction of developing B lymphocytes make it through heavy chain
rearrangement?
2/3
what fraction of developing B lymphocytes make it through light chain
rearrangement?
85%
, why does Ig light chain rearrangement have a higher rate of success than Ig
heavy chain rearrangement?
the cell has two chances to successfully rearrange the heavy chain, but it has four
chances to rearrange the light chain
Identify the two CD markers that when used in combination, can identify B-1 B
lymphocytes
CD5 and CD19/20
clonal deletion
apoptosis is induced because B cells are responding to self antigen
clonal anergy
B cells become anergic because they are responding to soluble self antigen
anergic
lymphocyte does not respond to its antigen
lymphotoxin
provides life signal for follicular dendritic cells
BAFF
(B cell activating factor)
provides life-saving maturation signal to B cell
primary lymphoid follicle
B cells congregate and interact with follicular dendritic cells in the secondary lymphoid
organs
secondary lymphoid follicle
a primary follicle in which activated B cells form a germinal center
COMPLETE SOLUTIONS GRADED A++
pre-BCR
checkpoint in early B cell development that monitors the production of Ig-mu heavy
chain
surrogate light chain
VPreB + lambda5
RAG-1/RAG-2
recognize recombination sequences and make double stranded DNA cuts to initiate
recombination
CAM (cell adhesion molecule)
important signal for developing B cells in bone marrow
polyspecific antibody
have the ability to bind a number of different antigens
B-1 cells have them
cell stages of B cell development
stem cell --> pro-B cell --> pre-B cell --> immature B cell --> naive mature B cell
pro-B cell
earliest identifiable B lineage cell
pre-B cell
,has transient expression of pre-BCR, rearranged Ig heavy chain, begins V-J
rearrangement of light chain
immature B cell
have surface expression of productively rearranged heavy chain plus rearranged light
chain
why is it advantageous for a pre-B cell to undergo a burst of proliferation
following signaling through the pre-BCR?
the burst allows the pre-B cell to express rearranged mu heavy chain with surrogate
light chain
what stage during B cell development are RAG-1 and RAG-2 expressed?
Pro-B and Pre-B cells
the role of stromal cells during B cell development and where they are located in
the body
...
the two growth factors that support B cell development in the bone marrow
stem cell factor (SCF)
soluble cytokine growth factor IL-7
what fraction of developing B lymphocytes make it through heavy chain
rearrangement?
2/3
what fraction of developing B lymphocytes make it through light chain
rearrangement?
85%
, why does Ig light chain rearrangement have a higher rate of success than Ig
heavy chain rearrangement?
the cell has two chances to successfully rearrange the heavy chain, but it has four
chances to rearrange the light chain
Identify the two CD markers that when used in combination, can identify B-1 B
lymphocytes
CD5 and CD19/20
clonal deletion
apoptosis is induced because B cells are responding to self antigen
clonal anergy
B cells become anergic because they are responding to soluble self antigen
anergic
lymphocyte does not respond to its antigen
lymphotoxin
provides life signal for follicular dendritic cells
BAFF
(B cell activating factor)
provides life-saving maturation signal to B cell
primary lymphoid follicle
B cells congregate and interact with follicular dendritic cells in the secondary lymphoid
organs
secondary lymphoid follicle
a primary follicle in which activated B cells form a germinal center
