BLD 313 STUDY EXAM QUESTIONS AND ANSWERS WITH
COMPLETE SOLUTIONS VERIFIED
How will you prepare 120 g of 35.0 %w/w sodium hydroxide (NaOH) aqueous
solution
Mix 42 g of NaOH and 78 g of deionized water and stir until all dissolve
You need to make 70.0 %v/v isopropanol aqueous solution. How many mL of
isopropanol (70.0 %v/v) aqueous solution can you make maximum if you only
have 250 mL of pure isopropanol
357 mL
How will you prepare 4.0 L of 0.85 %w/v sodium chloride (NaCl) aqueous solution.
Dissolve 34 g of NaCl in deionized water and make the final volume to be 4.0 L
You have to make 1.00 L of 500 mM MgCl2 with deionized water and magnesium
chloride anhydrous (MgCl2). How do you prepare this solution
Dissolved 47.6 g of MgCl2 into deionized water, and make the final volume 1.00 L
What is the osmolarity of 350 mM MgCl2 aqueous solution?
1.05 Osm/L
What are the normality of these two base aqueous solutions and two acid
aqueous solutions listed on the right? Match the correct normality.
3.3 M HCl
2.8 M NaOH
,2.5M H3PO4
1.5 M Ba(OH)2
1. 2.8 N
2. 3.0 N
3. 3.3 N
4. 7.5 N
(3) 3.3 M HCl
(1) 2.8 M NaOH
(4) 2.5M H3PO4
(2) 1.5 M Ba(OH)2
You diluted your patient's serum 100 μL with 400 μL isotonic saline solution.
What is the final dilution of this? Choose all the correct answer.
1:5 dilution
1/5 dilution
You need to find out the titer of analyte-X for patient-Y. You performed a serial
dilution of patient-Y serum and run a reaction with each dilution level as in the
table. You recorded the reaction result as (+) if there was a reaction: as (-) if there
was no reaction with the dilution level. What is the titer? ( Tube 5 is positive and
Tube 6 is negative)
32
Calculate the pH of each strong acid and strong base aqueous solutions. Assume
anion(s) and cation(s) will dissociate 100 %. The temperature of these solution is
, 25 ºC. The list of acid/base solution is on the right. Match the pH from the list on
the left.
0.50 M NaOH
0.20 M HCl
20 mM HCl
1. pH 0.70
2. pH 13.7
3. pH 0.30
4. pH 1.70
5. pH -1.30
(2) 0.50 M NaOH
(1) 0.20 M HCl
(4) 20 mM HCl
2-acetoxybenzoic acid (aspirin) is a weak acid (pKa = 3.48). What would be the pH
of 10 mM aspirin aqueous solution?
pH 2.74
Sodium acetate CH3COONa (0.001 moles) and acetic acid CH3COOH (0.030
moles) were used to make 1 L aqueous buffer solution. What is the final pH of this
buffer solution?
Acetic acid pKa = 4.76
pH 3.52
COMPLETE SOLUTIONS VERIFIED
How will you prepare 120 g of 35.0 %w/w sodium hydroxide (NaOH) aqueous
solution
Mix 42 g of NaOH and 78 g of deionized water and stir until all dissolve
You need to make 70.0 %v/v isopropanol aqueous solution. How many mL of
isopropanol (70.0 %v/v) aqueous solution can you make maximum if you only
have 250 mL of pure isopropanol
357 mL
How will you prepare 4.0 L of 0.85 %w/v sodium chloride (NaCl) aqueous solution.
Dissolve 34 g of NaCl in deionized water and make the final volume to be 4.0 L
You have to make 1.00 L of 500 mM MgCl2 with deionized water and magnesium
chloride anhydrous (MgCl2). How do you prepare this solution
Dissolved 47.6 g of MgCl2 into deionized water, and make the final volume 1.00 L
What is the osmolarity of 350 mM MgCl2 aqueous solution?
1.05 Osm/L
What are the normality of these two base aqueous solutions and two acid
aqueous solutions listed on the right? Match the correct normality.
3.3 M HCl
2.8 M NaOH
,2.5M H3PO4
1.5 M Ba(OH)2
1. 2.8 N
2. 3.0 N
3. 3.3 N
4. 7.5 N
(3) 3.3 M HCl
(1) 2.8 M NaOH
(4) 2.5M H3PO4
(2) 1.5 M Ba(OH)2
You diluted your patient's serum 100 μL with 400 μL isotonic saline solution.
What is the final dilution of this? Choose all the correct answer.
1:5 dilution
1/5 dilution
You need to find out the titer of analyte-X for patient-Y. You performed a serial
dilution of patient-Y serum and run a reaction with each dilution level as in the
table. You recorded the reaction result as (+) if there was a reaction: as (-) if there
was no reaction with the dilution level. What is the titer? ( Tube 5 is positive and
Tube 6 is negative)
32
Calculate the pH of each strong acid and strong base aqueous solutions. Assume
anion(s) and cation(s) will dissociate 100 %. The temperature of these solution is
, 25 ºC. The list of acid/base solution is on the right. Match the pH from the list on
the left.
0.50 M NaOH
0.20 M HCl
20 mM HCl
1. pH 0.70
2. pH 13.7
3. pH 0.30
4. pH 1.70
5. pH -1.30
(2) 0.50 M NaOH
(1) 0.20 M HCl
(4) 20 mM HCl
2-acetoxybenzoic acid (aspirin) is a weak acid (pKa = 3.48). What would be the pH
of 10 mM aspirin aqueous solution?
pH 2.74
Sodium acetate CH3COONa (0.001 moles) and acetic acid CH3COOH (0.030
moles) were used to make 1 L aqueous buffer solution. What is the final pH of this
buffer solution?
Acetic acid pKa = 4.76
pH 3.52
