BLD 313 QUIZ 6 EXAM QUESTIONS AND ANSWERS WITH COMPLETE SOLUTIONS VERIFIED

BLD 313 QUIZ 6 EXAM QUESTIONS AND ANSWERS WITH COMPLETE SOLUTIONS VERIFIED Which of the following is associated with chemiluminescence? Light emission produced due to electron excitation by chemical energy Which of the following best describes bioluminescence? Enzymatic oxidation of a substrate produces light emission Luminescence is caused by which of the following? Photons emitted while excited electrons drop down Which of the following statement is true about luminescent assays? Total light output is proportional to concentration of analyte in reaction dependent assays. Luciferase assay for ATP is an example of which type of assay? Luminescence assay The Bessey method for the determination of alkaline phosphatase takes advantage of substrate p-nitrophenyl forming the colored product p-nitrophenoxide (403nm). To 2.40ml of substrate containing buffer is added 0.10 ml of serum. After 5 minutes, the absorbance at 403nm has changed by 0.550. The molar absorptivity (ϵ) of the product is 18.8 x 103 L/mol ּּּcּm. What is the activity of this enzyme in U/L? 148 The Bessey method for the determination of alkaline phosphatase takes advantage of substrate p-nitrophenyl forming the colored product p-nitrophenoxide (403nm). To 2.40ml of substrate containing buffer is added 0.60 ml of serum. After 5 minutes, the absorbance at 403nm has changed by 0.550. The molar absorptivity (ϵ) of the product is 18.8 x 103 L/mol ּּּcּm. What is the activity of this enzyme in U/L? 29.5