BLD 313 EXAM 3 QUIZZES EXAM QUESTIONS AND ANSWERS
WITH COMPLETE SOLUTIONS VERIFIED
it is based on the wavelength we need to detect
A cuvette of absorption spectrophotometry can be made of polystyrene (plastic), glass,
quartz, etc. How do we choose the type of cuvette?
a. It depends on the specificity ans sensitivity of the method
b. It is based on the size of cuvette
c. It does not matter which one to use but the cost is different
d. It is based on the wavelength we need to detect
narrow the band pass
Which of the following can be done to increase the specificity as well as the sensitivity
of spectrophotometric analysis?
a. Broaden the band pass
b. Narrow the band pass
, c. Zeroing absorbance after changing the wavelength
d. Choose a proper material of cuvette
Increased specific and increased sensitivity
Which of the following represents a primary advantage of performing fluorometric over
absorption spectroscopic methods of analysis?
a. Easy to performing assays
b. There is not any extra advantage in use of fluorometric assay over absorption
spectroscopic assay.
c. Increased specificity and increased sensitivity
d. Cheaper light source
Prevent excitation light from reaching the detector
Flurometers are designed so that the path of the exciting light is at a right angle to the
path of the emitted light. What is the purpose of this design?
a. Prevent excitation light from reaching the detector
b. Focus both emitted and excitation light upon the detector
WITH COMPLETE SOLUTIONS VERIFIED
it is based on the wavelength we need to detect
A cuvette of absorption spectrophotometry can be made of polystyrene (plastic), glass,
quartz, etc. How do we choose the type of cuvette?
a. It depends on the specificity ans sensitivity of the method
b. It is based on the size of cuvette
c. It does not matter which one to use but the cost is different
d. It is based on the wavelength we need to detect
narrow the band pass
Which of the following can be done to increase the specificity as well as the sensitivity
of spectrophotometric analysis?
a. Broaden the band pass
b. Narrow the band pass
, c. Zeroing absorbance after changing the wavelength
d. Choose a proper material of cuvette
Increased specific and increased sensitivity
Which of the following represents a primary advantage of performing fluorometric over
absorption spectroscopic methods of analysis?
a. Easy to performing assays
b. There is not any extra advantage in use of fluorometric assay over absorption
spectroscopic assay.
c. Increased specificity and increased sensitivity
d. Cheaper light source
Prevent excitation light from reaching the detector
Flurometers are designed so that the path of the exciting light is at a right angle to the
path of the emitted light. What is the purpose of this design?
a. Prevent excitation light from reaching the detector
b. Focus both emitted and excitation light upon the detector
