BLD 313 EXAM 3 QUESTIONS WITH DETAILED
SOLUTIONS LATEST UPDATE 100% GUARANTEED PASS
What will be detected during absorption spectroscopy
Less transmitted light due to absorption
During absorption spectroscopy where should the detector be relative to the light
and cuvette
Straight line light, cuvette, detector
What will be detected during fluorometry
fluorescence light, which was emitted when the incident light excites it
During fluorometry where should the detector be relative to the light and cuvette
light, cuvette, 90 degree angle, detector
- to avoid original light
what will be detected by the turbidimeter
reduction of transmitted light due to scatter
during turbidimeter where should the detector be relative to the light and cuvette
straight line- Light, cuvette (with scatter), detector
what will be detected by the nephelometer
scattered light
during nephelometer where should the detector be relative to the light and the
cuvette
The angle of the detector depends on the analyze (usually 180 but not always)
, Key points of enzyme multiplied immunoassay technique (EMIT)
- Reagent Ag is near the enzyme active site
- Enzyme can not work when Ab binds to the Ag
Key points of Apoenzyme reactivation immunoassay (ARIS)
- reagent Ag is labeled with coenzyme (cofactor)
- enzyme can not work when Ab binds to the Ag
Key points of Cloned-enzyme donor immunoassay (CEDIA)
- part of enzyme is missing
- reagent Ag is on the missing part
- enzyme can not work whenAb prevent the missing part from coming back
Qualitative Data
- presence or absence: positive or negative
- Physical characteristics:
1. Color: gram stain
2. Shape: RBC shape
3. Other: cloudy, etc.
Semi-quantitive data
- an estimate of concentration
- based on an arbitrary scale:
1. 1+, 2+, 3+
2. 0-5, 5-10, 10-20, 30-50, >50
Quantitive data
SOLUTIONS LATEST UPDATE 100% GUARANTEED PASS
What will be detected during absorption spectroscopy
Less transmitted light due to absorption
During absorption spectroscopy where should the detector be relative to the light
and cuvette
Straight line light, cuvette, detector
What will be detected during fluorometry
fluorescence light, which was emitted when the incident light excites it
During fluorometry where should the detector be relative to the light and cuvette
light, cuvette, 90 degree angle, detector
- to avoid original light
what will be detected by the turbidimeter
reduction of transmitted light due to scatter
during turbidimeter where should the detector be relative to the light and cuvette
straight line- Light, cuvette (with scatter), detector
what will be detected by the nephelometer
scattered light
during nephelometer where should the detector be relative to the light and the
cuvette
The angle of the detector depends on the analyze (usually 180 but not always)
, Key points of enzyme multiplied immunoassay technique (EMIT)
- Reagent Ag is near the enzyme active site
- Enzyme can not work when Ab binds to the Ag
Key points of Apoenzyme reactivation immunoassay (ARIS)
- reagent Ag is labeled with coenzyme (cofactor)
- enzyme can not work when Ab binds to the Ag
Key points of Cloned-enzyme donor immunoassay (CEDIA)
- part of enzyme is missing
- reagent Ag is on the missing part
- enzyme can not work whenAb prevent the missing part from coming back
Qualitative Data
- presence or absence: positive or negative
- Physical characteristics:
1. Color: gram stain
2. Shape: RBC shape
3. Other: cloudy, etc.
Semi-quantitive data
- an estimate of concentration
- based on an arbitrary scale:
1. 1+, 2+, 3+
2. 0-5, 5-10, 10-20, 30-50, >50
Quantitive data
