GENETIC: EXAM 2 QUESTIONS AND
CORRECT ANSWERS
The Beadle and Tatum experiments were based on all
of the following assumptions except that _______.
two strains of auxotrophic Neurospora that grow on
minimal medium supplemented with biotin have mutations
in the same gene
In their first round of screening, Beadle and Tatum
plated spores on minimal medium. The purpose of this
screen was to _______.
determine whether any auxotrophic mutants had been
generated
Which of the following can be inferred from the Beadle
and Tatum experiments?
For a mutation resulting in the production of a defective
enzyme involved in a biosynthetic pathway, the compound
preceding the corresponding step will accumulate.
Which subunit of RNA polymerase establishes
template binding to a promoter in prokaryotes?
σ (sigma)
What are two main types of posttranscriptional
modifications that take place in the mRNA of
eukaryotes?
The addition of a 7-mG cap at the 5' end of the transcript
and the addition of a poly-A sequence at the 3' end of the
message.
, Which of the following contains the three
posttranscriptional modifications often seen in the
maturation of mRNA in eukaryotes?
5'-capping, 3'-poly(A) tail addition, splicing
Transcription factors function to help move ribosomes
along the mRNA.
False
A 3' poly-A tail and a 5'-cap are common components
of prokaryotic RNAs.
False
Which of the following is a characteristic of RNA
splicing in Eukaryotes?
Exon/intron boundaries are typically characterized by a 5'
GU splice junction and a 3' AG splice junction.
A snRNP is best described as _______.
small RNAs associated with protein complexes in the
nucleus
Which of the following is most likely attributable to a
base substitution at a 5' splice junction?
A longer than usual final transcript
In which cellular organelle do the three
posttranscriptional modifications often seen in the
maturation of mRNA in eukaryotes occur?
Nucleus
Which of the following DNA sequences is one strand
of a restriction enzyme recognition sequence?
5' GGATCC 3'
X-Gal is included in the growth medium on which cells
transformed with bacterial plasmids are grown. The
reason X-Gal is included is to _______.
identify bacteria that contain a recombinant plasmid
CORRECT ANSWERS
The Beadle and Tatum experiments were based on all
of the following assumptions except that _______.
two strains of auxotrophic Neurospora that grow on
minimal medium supplemented with biotin have mutations
in the same gene
In their first round of screening, Beadle and Tatum
plated spores on minimal medium. The purpose of this
screen was to _______.
determine whether any auxotrophic mutants had been
generated
Which of the following can be inferred from the Beadle
and Tatum experiments?
For a mutation resulting in the production of a defective
enzyme involved in a biosynthetic pathway, the compound
preceding the corresponding step will accumulate.
Which subunit of RNA polymerase establishes
template binding to a promoter in prokaryotes?
σ (sigma)
What are two main types of posttranscriptional
modifications that take place in the mRNA of
eukaryotes?
The addition of a 7-mG cap at the 5' end of the transcript
and the addition of a poly-A sequence at the 3' end of the
message.
, Which of the following contains the three
posttranscriptional modifications often seen in the
maturation of mRNA in eukaryotes?
5'-capping, 3'-poly(A) tail addition, splicing
Transcription factors function to help move ribosomes
along the mRNA.
False
A 3' poly-A tail and a 5'-cap are common components
of prokaryotic RNAs.
False
Which of the following is a characteristic of RNA
splicing in Eukaryotes?
Exon/intron boundaries are typically characterized by a 5'
GU splice junction and a 3' AG splice junction.
A snRNP is best described as _______.
small RNAs associated with protein complexes in the
nucleus
Which of the following is most likely attributable to a
base substitution at a 5' splice junction?
A longer than usual final transcript
In which cellular organelle do the three
posttranscriptional modifications often seen in the
maturation of mRNA in eukaryotes occur?
Nucleus
Which of the following DNA sequences is one strand
of a restriction enzyme recognition sequence?
5' GGATCC 3'
X-Gal is included in the growth medium on which cells
transformed with bacterial plasmids are grown. The
reason X-Gal is included is to _______.
identify bacteria that contain a recombinant plasmid
