LABORATORY BIOSAFETY - Answers IT IS PROTECTION FOR WORKERS
LABORATORY BIOSECURITY - Answers PROTECTION FOR MICROORGANISM OR PATHOGENS
LABORATORY BIOSAFETY - Answers are containment principles, technologies, and practices
implemented to prevent unintentional exposure to pathogens and toxins, or their unintentional release
BIOSECURITY - Answers are protection, control and accountability for valuable biological materials
within laboratories, in order to prevent their unauthorized access, loss, theft, misuse, diversion or
intentional release
RISK GROUP 1 - Answers Unlikely to cause harm or disease on healthy individuals
RISK GROUP 2 - Answers •Can cause disease but moderate or less serious hazard
•Preventive measures and medications available
RISK GROUP 3 - Answers •Can cause serious disease but not ordinarily spread
•Preventive measures and medications available
RISK GROUP 4 - Answers •NO MEDICATIONS, causes serious disease and easy mode of transmission
Pathogenicity of the organism
Mode of transmission, host range, immunity, vectors and environment
Local availability of preventive measures
Local availability of effective treatment - Answers Classification of risk groups may vary from one country
to another depending on:
Biosafety Level 1 - Answers suitable for work involving not known to cause disease; minimal potential
hazard to laboratory personnel
Biosafety Level 2 - Answers similar to Level 1; moderate potential hazard
Biosafety Level 3 - Answers done with indigenous or exotic agents which may cause serious or
potentially lethal disease after inhalation
Biosafety Level 4 - Answers work with dangerous and exotic agents that pose a high risk of aerosol-
transmitted laboratory infections; may cause severe to fatal disease in humans, vaccines or other
treatments are not available
HIGH EFFICIENCY PARTICULATE AIR FILTERS - Answers DEFINITION OF HEPA FILTER
HEPA FILTER - Answers Remove particles,including microorganisms, from the air
, Biological safety cabinet - Answers it use hepa filter to treat air --inflow / exhaust
class 1 , 2, 3 - Answers 3 types of biological safety cabinet
Class I - Answers provide worker and environmental protection, but no product protection. exhaust air
filtered
Class II - Answers filter both exhaust and intake air to protect the worker and the environment from the
contamination as well as to protect product in the cabinet. suitable for micoorganisms assigned to bio-
safety level 1, 2, 3..
Class III - Answers gas tight provides the highest attainable level of protection to personnel and the
environment.
Level 1, 2 ,3, 4 - Answers BIOSAFETY LEVELS
Biosafety level I - Answers types of agent: those not known to cause disease in healthy adults
Biosafety Level II - Answers Type of agent: common human pathogens
Biosafety level III - Answers Those that may cause serious or lethal disease via inhalation. Effective
treatment available
Biosafety level IV - Answers those that pose high risk of life-threatening disease. may be transmitted by
aerosols. No vaccine or theraphy
Bacillus subtilis, Myobacterium gordonae, soil microbes - Answers Example agents for Biosafety level I
E.coli, Salmonella, HIV, HVB, Influenza - Answers Example agents for Biosafety level II
Bacillus anthracis, Francisella, Brucella, Myobacterium tubercolosis, Rickettsia rickettsii, Coxiella burnetii,
mold stages of systemic fungi - Answers Example agents for Biosafety level III
Ebola virus, Lassa virus, others that cause hemorrhagic fevers - Answers Example agents for Biosafety
level IV
Category A, B, C - Answers CLassification of Biological agents
Category A - Answers Easily disseminated or transmitted from person to person
Category B - Answers Moderately easy to dessiminate
Category C - Answers Could be engineered for mass dessimination
Category A - Answers Impact: High motility, potential for major public health impact
Category B - Answers Moderate illness, low death rate
Category C - Answers High morbidity/mortality, major public health impact