ASCP CG CERTIFICATION EXAMS VERIFIED QUESTIONS AND
CORRECT ANSWERS
"Starring-in" is a method of
- ANSWERS-Immobilizing the tissue in the culture vessel
24 hours after culture initiation, a single dish from an amniotic fluid turns cloudy. Which cultures should be
disposed?
- ANSWERS-The single cloudy dish only
46,XY,der(9)(t(9;10)(q32;q12). What term best describes this written karyotype?
- ANSWERS-Unbalanced
("der" means "a derivative chromosome"; "t" translocation. This indi. has only one copy of chromosome 9q
from band 9q33 to 9qter), therefore is unbalanced
50% of cells from an amniotic flask have 92,XXYY and the other 50% have 46,XY. This probably
indicated:
- ANSWERS-Pseudomosaicism
A blood sample can be used to study the cytogenetics of a malignancy only if:
- ANSWERS-Dividing leukemic cells are present in circulation
A bone marrow sample collected in transport medium is leaking into the sealed plastic bag in which it was
transported. The technologist should_______.
- ANSWERS-Clean up the tube while wearing gloves
A change of 1 pH unit in culture medium is caused by a ________ change in CO2 concentration.
- ANSWERS-10-fold
A Cri-du-chat patient has a chromosome abnormality of:
- ANSWERS-r(5)(p15.1q35)
,(this ring chromosome is missing the tip end of 5p and the tip end of 5q. Deletion of 5p15 is cri-du-chat
syndrome)
A fetus with an increased risk for trisomy 18 would have a serum analytes that is:
- ANSWERS-low AFP, low hCG, and low uE3
AFP = Alpha feto protein
hCG = human chorionic gonadotropin
uE3 = Unconjugated estriol
A laboratory' quality assurance program includes _______________.
- ANSWERS-Proficiency testing
A MLL FISH gave the following signal pattern 1F 1R 1G. What is the ISCN of this cell?
- ANSWERS-nuc ish (MLLx2)(5?MLL sep 3?MLLx1)
A new hypotonic solution causes overspreading of chromosomes. What technique can be used to prevent
the overspreading?
- ANSWERS-Reduce the drying time
A regulatory inspecter requests a copy of a lab patient's record for his inspection files, the tech should:
- ANSWERS-Deny the inspector a copy of the record
See HIPAA compliance policy
A tech suspects an inversion of chrom 9 with G-banding analysis. What banding technique would confirm
this?
- ANSWERS-C-banding
A technologist wants to stain chromosomes using a banding technique that manifests AT-rich regions on
chromosomes. What staining procedure should he use?
- ANSWERS-QFQ
According to CAP regulations, what is the minimum band level for consitutional specimens?
- ANSWERS-400
, The suggested level for most constitutional specimen is 550, but 400 is the lowest acceptible level
See CAP regulation CYG.42200
After G-banded analysis, a cyto director suspects chrom 10 has a terminal del. What banding procedure
would be MOST helpful in determining if a deletion has occurred?
- ANSWERS-FISH
After performing koehler illumination, what other scope part should be adjusted to ensure optimal contrast
between light and dark bands of the chroms?
- ANSWERS-The condenser diaphragm should be closed down by 1/4 to 1/3
After staining, the chromosomes appear to have indistinct bands with little contrast. The problem is
corrected by:
- ANSWERS-Increasing trypsin exposure (chroms were muddy and dark)
All of the cultures in a CO2 incubator failed, what aspects of the incubator are important to monitor to
assess the source of the problem?
- ANSWERS-Temperature,
CO2 level, and
Humidity level
All of the following ARE characteristics of the horizontal laminar flow hood?
- ANSWERS-Protects the culture from contamination
Should not be used with transformed cell lines.
Sterlizes the air entering the hood
All of the following chromosomes have dark bands on chromosome regions that can be used as indicators
when preparing the best image:
- ANSWERS-Chromosome 11p
Chromosome 12q
Chromosome 17q
CORRECT ANSWERS
"Starring-in" is a method of
- ANSWERS-Immobilizing the tissue in the culture vessel
24 hours after culture initiation, a single dish from an amniotic fluid turns cloudy. Which cultures should be
disposed?
- ANSWERS-The single cloudy dish only
46,XY,der(9)(t(9;10)(q32;q12). What term best describes this written karyotype?
- ANSWERS-Unbalanced
("der" means "a derivative chromosome"; "t" translocation. This indi. has only one copy of chromosome 9q
from band 9q33 to 9qter), therefore is unbalanced
50% of cells from an amniotic flask have 92,XXYY and the other 50% have 46,XY. This probably
indicated:
- ANSWERS-Pseudomosaicism
A blood sample can be used to study the cytogenetics of a malignancy only if:
- ANSWERS-Dividing leukemic cells are present in circulation
A bone marrow sample collected in transport medium is leaking into the sealed plastic bag in which it was
transported. The technologist should_______.
- ANSWERS-Clean up the tube while wearing gloves
A change of 1 pH unit in culture medium is caused by a ________ change in CO2 concentration.
- ANSWERS-10-fold
A Cri-du-chat patient has a chromosome abnormality of:
- ANSWERS-r(5)(p15.1q35)
,(this ring chromosome is missing the tip end of 5p and the tip end of 5q. Deletion of 5p15 is cri-du-chat
syndrome)
A fetus with an increased risk for trisomy 18 would have a serum analytes that is:
- ANSWERS-low AFP, low hCG, and low uE3
AFP = Alpha feto protein
hCG = human chorionic gonadotropin
uE3 = Unconjugated estriol
A laboratory' quality assurance program includes _______________.
- ANSWERS-Proficiency testing
A MLL FISH gave the following signal pattern 1F 1R 1G. What is the ISCN of this cell?
- ANSWERS-nuc ish (MLLx2)(5?MLL sep 3?MLLx1)
A new hypotonic solution causes overspreading of chromosomes. What technique can be used to prevent
the overspreading?
- ANSWERS-Reduce the drying time
A regulatory inspecter requests a copy of a lab patient's record for his inspection files, the tech should:
- ANSWERS-Deny the inspector a copy of the record
See HIPAA compliance policy
A tech suspects an inversion of chrom 9 with G-banding analysis. What banding technique would confirm
this?
- ANSWERS-C-banding
A technologist wants to stain chromosomes using a banding technique that manifests AT-rich regions on
chromosomes. What staining procedure should he use?
- ANSWERS-QFQ
According to CAP regulations, what is the minimum band level for consitutional specimens?
- ANSWERS-400
, The suggested level for most constitutional specimen is 550, but 400 is the lowest acceptible level
See CAP regulation CYG.42200
After G-banded analysis, a cyto director suspects chrom 10 has a terminal del. What banding procedure
would be MOST helpful in determining if a deletion has occurred?
- ANSWERS-FISH
After performing koehler illumination, what other scope part should be adjusted to ensure optimal contrast
between light and dark bands of the chroms?
- ANSWERS-The condenser diaphragm should be closed down by 1/4 to 1/3
After staining, the chromosomes appear to have indistinct bands with little contrast. The problem is
corrected by:
- ANSWERS-Increasing trypsin exposure (chroms were muddy and dark)
All of the cultures in a CO2 incubator failed, what aspects of the incubator are important to monitor to
assess the source of the problem?
- ANSWERS-Temperature,
CO2 level, and
Humidity level
All of the following ARE characteristics of the horizontal laminar flow hood?
- ANSWERS-Protects the culture from contamination
Should not be used with transformed cell lines.
Sterlizes the air entering the hood
All of the following chromosomes have dark bands on chromosome regions that can be used as indicators
when preparing the best image:
- ANSWERS-Chromosome 11p
Chromosome 12q
Chromosome 17q
