BIOL 101 QUESTIONS AND ANSWERS EXAM LATEST
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Define Biotechnology
the manipulation of organisms or their genetic components to make useful products
What is the most common genetically modified trait found in Canadian crops?
Herbicide resistance
Define Genetic Engineering
The direct manipulation of genes for practical purposes.
Manipulation of genes usually require Cloning.
Define Cloning
refers to any procedure that recreates a genetic replica of a cell or organism.
Define Recombinant DNA
DNA from two different sources that is combined into one DNA molecule.
What 4 steps comprise Genetic Modification?
1. Copy the gene.
2. Cut the plasmid.
3. Join the two pieces.
4. Introduce the plasmid into bacteria.
Define Polymerase Chain Reaction (PCR)
The mixing of a DNA template, DNA polymerase, dNTPS (dATP, dCTP, dTTP, dGTP),
and primers (single stranded DNA);
dNTPS synthesize the new DNA strands and Primers match the template of interest
Define Denaturation in DNA cloning
The separation of the two strands of DNA.
What temperature is DNA denatured at?
94 Degrees Celsius
What does Annealing do in DNA cloning?
Annealing causes the mixture to favour the binding of the primers to the two DNA
strands of the DNA template.
What serves as a starting point for the new DNA strand in DNA cloning?
The primers serve as a starting point for the new DNA strand.
What temperature does annealing occur at?
50 to 70 degrees Celsius
What happens in Polymerization in DNA cloning?
Polymerization involves the DNA polymerase binding to the endpoint of the primer and
extends the DNA strands by adding in dNTPS.
If one wanted to making more DNA copies, what step is returned to resume the
cloning process?
Denaturation
What are the 4 steps in Gel Electrophoresis
1. DNA is loaded into agarose gel.
2. Electric current is passed through.
UPDATE.Buy Quality Materials!
Define Biotechnology
the manipulation of organisms or their genetic components to make useful products
What is the most common genetically modified trait found in Canadian crops?
Herbicide resistance
Define Genetic Engineering
The direct manipulation of genes for practical purposes.
Manipulation of genes usually require Cloning.
Define Cloning
refers to any procedure that recreates a genetic replica of a cell or organism.
Define Recombinant DNA
DNA from two different sources that is combined into one DNA molecule.
What 4 steps comprise Genetic Modification?
1. Copy the gene.
2. Cut the plasmid.
3. Join the two pieces.
4. Introduce the plasmid into bacteria.
Define Polymerase Chain Reaction (PCR)
The mixing of a DNA template, DNA polymerase, dNTPS (dATP, dCTP, dTTP, dGTP),
and primers (single stranded DNA);
dNTPS synthesize the new DNA strands and Primers match the template of interest
Define Denaturation in DNA cloning
The separation of the two strands of DNA.
What temperature is DNA denatured at?
94 Degrees Celsius
What does Annealing do in DNA cloning?
Annealing causes the mixture to favour the binding of the primers to the two DNA
strands of the DNA template.
What serves as a starting point for the new DNA strand in DNA cloning?
The primers serve as a starting point for the new DNA strand.
What temperature does annealing occur at?
50 to 70 degrees Celsius
What happens in Polymerization in DNA cloning?
Polymerization involves the DNA polymerase binding to the endpoint of the primer and
extends the DNA strands by adding in dNTPS.
If one wanted to making more DNA copies, what step is returned to resume the
cloning process?
Denaturation
What are the 4 steps in Gel Electrophoresis
1. DNA is loaded into agarose gel.
2. Electric current is passed through.
