Test Bank For Microbiology: The Human Experience 2nd E
f f f f f f f f
dition By John W Foster, Zarrintaj Aliabadi, Joan L Sloncze
f f f f f f f f f
wski
Whatfisfthefconceptfoffspontaneousfgeneration?f-
fANSWERffflivingf organismsf couldfdevelopffromfnonlivingf orfdecomposingfmatter
Whofchallengedfthefconceptfoffspontaneousfgenerationfbyfshowingfthatfmaggotsfonfdec
ayingfmeatfcameffromfflies,fnotffromfthefmeatfitself?f-fANSWERfffFrancescofRedi
Whofshowedfthatfmuttonfbrothfboiledfinfflasksfandfthenfsealedfcouldfstillfdevelopfmicroor
ganisms,fwhichfsupportedftheftheoryfoffspontaneousfgeneration?f-
fANSWERfffJohnf Needham
Whofshowedfthatfflasksfsealedfandfthenfboiledfhadfnofgrowthfoffmicroorganisms?f-
fANSWERfffLazzarofSpallanzani
Whofproposedfthatfairfcarriedfgerms?f-fANSWERfffSpallanzani
Whofdisprovedftheftheoryfoffspontaneousfgeneration?f-fANSWERfffLouisfPasteur
Whofdemonstratedfthatfdustfcarriedfmicrobesfandfprovedfevidencefforfthefexistencefoffh
eat-fresistantfformsfoffbacteria?f-fANSWERfffJohnfTyndall
Whofshowedfthatfafsilkwormfdiseasefwasfcausedfbyfaffungus?f-
fANSWERfffAgostinof Bassi
WhofdemonstratedfthatfthefGreatfPotatofBlightfwasfcausedfbyfafwaterfmold?f-
fANSWERfff M.f J.fBerkely
Whofshowedfthatfthefpebrinefdiseasefoffsilkwormsfwasfcausedfbyfafprotozoanfparasite?f
-fANSWERfffLouisfPasteur
Whofdevelopedfafsystemfoffsurgeryfdesignedftofpreventfmicroorganismsffromfenteringfw
ounds?f-fANSWERfffJohnfLister
Whoftransformedfthefpracticefoffsurgery?f-fANSWERfffJohnfLister
WhofestablishedfthefrelationshipfbetweenfBacillusfanthracisfandfanthrax?f-
fANSWERfffRobertf Koch
WhatfarefthefKochfPostulates?f-
fANSWERfff1.Thefmicroorganismsfmustfbef presentf inf everyf casef offthefdiseasefbutfabsen
tffromfhealthyfindividualsf
2.Thefsuspectedfmicroorganismsfmustfbefisolatedfandfgrownfinfpurefculturef
,3.fThefsamefdiseasefmustfresultfwhenfthefisolatedfmicroorganismfisfinoculatedfintofafhea
lthyfhostf
4.fThefsamefmicroorganismfmustfbefisolatedfagainffromfthefdiseasedfhost
LimitationsfoffKoch'sfPostulatesf-fANSWERfff-
Someforganismsfcannotfbefgrownfinfpurefculture
-Usingfhumansfinfcompletingfthefpostulatesfisfunethical
-Molecularfandfgeneticfevidencefmayfreplace
WhatfvaccinationfdidfEdwardfJennerfmake?f-fANSWERfffsmallpoxfvaccination
WhatfvaccinationsfdidfPasteurfproduce?f-
fANSWERfffchickenf cholera,f rabiesfandfanthrax
Whofinducedfformationfoffdippedftoxin-antitoxinsfrabbitsf-
fANSWERfffEmilf vonf Behringf andfShibasaburofKitasato
Whofshowedfthatfthefsilkwormfdiseasefwasfcausedfbyffungus?f-fANSWERfffBassi
WhofdemonstratedfthatfthefGreatfPotatofBlightfoffIrelandfwasfcausedfbyfafwaterfmold?f-
fANSWERfff M.J.fBerkeley
Whofshowedfthatfthefperbrinefdiseasefoffsilkwormsfwasfcausedfbyfafprotozoanfparasite?
f -fANSWERfffLouisf Pasteur
Afsimplefmorphologyfandflackfaftruefmembrane-delimitedfnucleusf-
fANSWERfffprokaryotes
Whatfisfafmorphologicallyfcomplexfandfhasfafmembrane-enclosedfnucleus?f-
fANSWERfffEucaryotes
Whatfletftofthefdiscoveryfoffvirusesfasfdiseasefcausingfagents?f-
fANSWERfffbacterialffilterfthatf removedfbacteriaf andf largerfmicrobesf(Charlesf Chamberl
and)
Theffivefkingdomfclassificationfschemefisf-
fANSWERfffthef MoneraforfProcaryotae,f Protista,f Fungi,f Animalia,fandfPlantae
Whatfarefribozymes?f-
fANSWERfffRNAfmoleculesf thatfformf peptidefbondsfandfperformf cellularf workf andf replica
tion
Thefthreefdomainfschemefisf-fANSWERfffBacteria,fArchaea,fandfEucarya
Thefthreefdomainfschemefisfbasedfon?f-fANSWERfffafcomparaisonfoffribosomalfRNA
Thefearliestfcellsfmayfhavefbeenf-fANSWERfffRNAfsurroundedfbyfliposomes
Whatfarefthefthreefbacterialfmorphology?f-fANSWERfff-bacillusf(littlefrod)
-coccusf(grainforfberry)
,-spirillumf(coiledforfhelical)
Whatfareftheftypesfofflightfmicroscopes?f-fANSWERfff-bright-fieldfmicroscope
-dark-fieldfmicroscope
-phase-contrastfmicroscope
-fluorescencefmicroscopes
Whatfisfafmeasurefoffhowfgreatlyfafsubstancefslowsfthefvelocityfofflight?f-
fANSWERfffRefractivef index
Thefdirectionfandfmagnitudefoffbendingflightfinfafmicroscopefisfdeterminedfbyfthef_____f-
fANSWERfff refractivef indexesf offthef twof mediafformingf thef interface
Whichfmicroscopefproducesfafdarkfimagefagainstfafbrighterfbackground?f-
fANSWERfffthefbright-fieldfmicroscope
Whatfisfthefproductfoffthefmagnificationsfoffthefocularflensfandfthefobjectiveflens?f-
fANSWERfffthef totalfmagnification
Whatfisftheftotalfmagnificationfoffthefbrightffield,fdarkffield,fandfphase-
contrastfmicroscopes?f-fANSWERfff2000x
Whatfisftheftotalfresolutionfoffthefbrightffield,fdarkffield,fandfphasefcontrastfmicroscopes?f
-fANSWERfff0.2um
Whatfisfthefpurposefoffthefbrightffieldfmicroscope?f-
fANSWERfffforf livef andfpreservedf stainedf specimens
Whatfisfthefpurposefoffthefdarkffieldfmicroscope?f-
fANSWERffftofobservef living,f unstainedfpreparations
Whichfmicroscopesfarefgoodfforfobservingflivingfcells?f-fANSWERfff-
ThefDifferentialfInterferencefContrastfMicroscope
-Phase-contrastfmicroscope
Whichfmicroscopefenhancesfthefcontrastfbetweenfintracellularfstructuresfhavingfslightfd
ifferencesfinfthefrefractivefindex?f-fANSWERfffphase-contrastfmicroscope
Whichfmicroscopefshowsfafbrightfimagefoffthefobjectfresultingffromftheffluorescentflightfe
mittedfbyfthefspecimen?f-fANSWERffftheffluorescencefmicroscope
Theffluorescencefmicroscopefexposesfthefspecimenftofwhatftypesfofflight?f-
fANSWERfffuv,f violet,f orfblue
Thefdistancefbetweenfthefcenterfofftheflensfandffocalfpoint?f-fANSWERffffocalflength
Thefshorterftheffocalfpoint,fthef______fthefmagnificationf-fANSWERfffgreater
Thefshorterfthefwavelength,fthef______fthefresolutionf-fANSWERfffgreater
, Thefdistancefbetweenftheffrontfsurfacefofflensfandfsurfacefoffcoverfglassfonfspecimenf-
fANSWERfffWorkingfdistance
Asfthefworkingfdistancefdecrease,ftheffieldfoffviewf_____f-fANSWERfffdecrease
Asftheffieldfoffviewfdecrease,ftheflightfgetsf____f-fANSWERfffdimmer
Whatfisfthefpurposefoffaddingfthefimmersionfoil?f-
fANSWERffftofobtainfmoref lightfbyfprovidingf thefsamefrefractionfenergyf asf glass
Asfthefmagnificationfincreases,fwhatfhappensftoftheffocalflength?f-
fANSWERfffitfdecrease
Whatfisfthefchargefoffafbacteriafcellfsurface?f-fANSWERfffnegativefcharge
Whatfisfthefchargefoffbasicfdyes?f-fANSWERfffpositivefcharge
Whatfisfthefchargefoffacidfdyes?f-fANSWERfffnegativefcharge
Afthin,funiformffilmfoffbacterialfgrowthfonfafglassfslidefinforderftofproceedfwithffurtherfstain
ingfforfmicroscopicfexaminationf-fANSWERfffsmear
Thefprocessfbyfwhichforganismfisfkilledfandffirmlyfattachedftofmicroscopefslidef-
fANSWERffffixation
Heatffixationfvs.fChemicalffixingf(notfthefprocedure)f-fANSWERfff-
heatffixationfpreservesfoverallfshapefbutfnotfinternalfstructures
-
chemicalffixingfprotectsffinefcellularfstructurefandfshapefofflarger,fmorefdelicateforganis
ms
Whatfinformationfdoesfdifferentialfstainingfprovide?f-
fANSWERfffdividesfmicroorganismsf intof groupsfbasedf onf theirf stainingfproperties
Whatfisfthefmostfwidelyfusedfdifferentialfstainingfprocedure?f-fANSWERfffgramfstaining
Negativefstainingf-fANSWERfffthefbacteriafisfnotfstained;fthefbackgroundfisfstained
Whatfisfshadowingfinfelectronfmicroscopy?f-
fANSWERfffcoatingf specimenf withfaf thinffilmf offheavyf metal
WhatfisftheftotalfmagnificationfoffthefTransmissionfElectronfMicroscopefandfthefScannin
gfElectronfMicroscope?f-fANSWERfff1,000,000x
WhatfisftheftotalfmagnificationfoffthefScanningfElectronfMicroscope?f-
fANSWERfff1,000,000x
Whichfmicroscopefusesfelectronsfreflectedffromfthefsurfacefoffafspecimenftofcreatefanfi
mage?f-fANSWERfffscanningfelectronfmicroscope
f f f f f f f f
dition By John W Foster, Zarrintaj Aliabadi, Joan L Sloncze
f f f f f f f f f
wski
Whatfisfthefconceptfoffspontaneousfgeneration?f-
fANSWERffflivingf organismsf couldfdevelopffromfnonlivingf orfdecomposingfmatter
Whofchallengedfthefconceptfoffspontaneousfgenerationfbyfshowingfthatfmaggotsfonfdec
ayingfmeatfcameffromfflies,fnotffromfthefmeatfitself?f-fANSWERfffFrancescofRedi
Whofshowedfthatfmuttonfbrothfboiledfinfflasksfandfthenfsealedfcouldfstillfdevelopfmicroor
ganisms,fwhichfsupportedftheftheoryfoffspontaneousfgeneration?f-
fANSWERfffJohnf Needham
Whofshowedfthatfflasksfsealedfandfthenfboiledfhadfnofgrowthfoffmicroorganisms?f-
fANSWERfffLazzarofSpallanzani
Whofproposedfthatfairfcarriedfgerms?f-fANSWERfffSpallanzani
Whofdisprovedftheftheoryfoffspontaneousfgeneration?f-fANSWERfffLouisfPasteur
Whofdemonstratedfthatfdustfcarriedfmicrobesfandfprovedfevidencefforfthefexistencefoffh
eat-fresistantfformsfoffbacteria?f-fANSWERfffJohnfTyndall
Whofshowedfthatfafsilkwormfdiseasefwasfcausedfbyfaffungus?f-
fANSWERfffAgostinof Bassi
WhofdemonstratedfthatfthefGreatfPotatofBlightfwasfcausedfbyfafwaterfmold?f-
fANSWERfff M.f J.fBerkely
Whofshowedfthatfthefpebrinefdiseasefoffsilkwormsfwasfcausedfbyfafprotozoanfparasite?f
-fANSWERfffLouisfPasteur
Whofdevelopedfafsystemfoffsurgeryfdesignedftofpreventfmicroorganismsffromfenteringfw
ounds?f-fANSWERfffJohnfLister
Whoftransformedfthefpracticefoffsurgery?f-fANSWERfffJohnfLister
WhofestablishedfthefrelationshipfbetweenfBacillusfanthracisfandfanthrax?f-
fANSWERfffRobertf Koch
WhatfarefthefKochfPostulates?f-
fANSWERfff1.Thefmicroorganismsfmustfbef presentf inf everyf casef offthefdiseasefbutfabsen
tffromfhealthyfindividualsf
2.Thefsuspectedfmicroorganismsfmustfbefisolatedfandfgrownfinfpurefculturef
,3.fThefsamefdiseasefmustfresultfwhenfthefisolatedfmicroorganismfisfinoculatedfintofafhea
lthyfhostf
4.fThefsamefmicroorganismfmustfbefisolatedfagainffromfthefdiseasedfhost
LimitationsfoffKoch'sfPostulatesf-fANSWERfff-
Someforganismsfcannotfbefgrownfinfpurefculture
-Usingfhumansfinfcompletingfthefpostulatesfisfunethical
-Molecularfandfgeneticfevidencefmayfreplace
WhatfvaccinationfdidfEdwardfJennerfmake?f-fANSWERfffsmallpoxfvaccination
WhatfvaccinationsfdidfPasteurfproduce?f-
fANSWERfffchickenf cholera,f rabiesfandfanthrax
Whofinducedfformationfoffdippedftoxin-antitoxinsfrabbitsf-
fANSWERfffEmilf vonf Behringf andfShibasaburofKitasato
Whofshowedfthatfthefsilkwormfdiseasefwasfcausedfbyffungus?f-fANSWERfffBassi
WhofdemonstratedfthatfthefGreatfPotatofBlightfoffIrelandfwasfcausedfbyfafwaterfmold?f-
fANSWERfff M.J.fBerkeley
Whofshowedfthatfthefperbrinefdiseasefoffsilkwormsfwasfcausedfbyfafprotozoanfparasite?
f -fANSWERfffLouisf Pasteur
Afsimplefmorphologyfandflackfaftruefmembrane-delimitedfnucleusf-
fANSWERfffprokaryotes
Whatfisfafmorphologicallyfcomplexfandfhasfafmembrane-enclosedfnucleus?f-
fANSWERfffEucaryotes
Whatfletftofthefdiscoveryfoffvirusesfasfdiseasefcausingfagents?f-
fANSWERfffbacterialffilterfthatf removedfbacteriaf andf largerfmicrobesf(Charlesf Chamberl
and)
Theffivefkingdomfclassificationfschemefisf-
fANSWERfffthef MoneraforfProcaryotae,f Protista,f Fungi,f Animalia,fandfPlantae
Whatfarefribozymes?f-
fANSWERfffRNAfmoleculesf thatfformf peptidefbondsfandfperformf cellularf workf andf replica
tion
Thefthreefdomainfschemefisf-fANSWERfffBacteria,fArchaea,fandfEucarya
Thefthreefdomainfschemefisfbasedfon?f-fANSWERfffafcomparaisonfoffribosomalfRNA
Thefearliestfcellsfmayfhavefbeenf-fANSWERfffRNAfsurroundedfbyfliposomes
Whatfarefthefthreefbacterialfmorphology?f-fANSWERfff-bacillusf(littlefrod)
-coccusf(grainforfberry)
,-spirillumf(coiledforfhelical)
Whatfareftheftypesfofflightfmicroscopes?f-fANSWERfff-bright-fieldfmicroscope
-dark-fieldfmicroscope
-phase-contrastfmicroscope
-fluorescencefmicroscopes
Whatfisfafmeasurefoffhowfgreatlyfafsubstancefslowsfthefvelocityfofflight?f-
fANSWERfffRefractivef index
Thefdirectionfandfmagnitudefoffbendingflightfinfafmicroscopefisfdeterminedfbyfthef_____f-
fANSWERfff refractivef indexesf offthef twof mediafformingf thef interface
Whichfmicroscopefproducesfafdarkfimagefagainstfafbrighterfbackground?f-
fANSWERfffthefbright-fieldfmicroscope
Whatfisfthefproductfoffthefmagnificationsfoffthefocularflensfandfthefobjectiveflens?f-
fANSWERfffthef totalfmagnification
Whatfisftheftotalfmagnificationfoffthefbrightffield,fdarkffield,fandfphase-
contrastfmicroscopes?f-fANSWERfff2000x
Whatfisftheftotalfresolutionfoffthefbrightffield,fdarkffield,fandfphasefcontrastfmicroscopes?f
-fANSWERfff0.2um
Whatfisfthefpurposefoffthefbrightffieldfmicroscope?f-
fANSWERfffforf livef andfpreservedf stainedf specimens
Whatfisfthefpurposefoffthefdarkffieldfmicroscope?f-
fANSWERffftofobservef living,f unstainedfpreparations
Whichfmicroscopesfarefgoodfforfobservingflivingfcells?f-fANSWERfff-
ThefDifferentialfInterferencefContrastfMicroscope
-Phase-contrastfmicroscope
Whichfmicroscopefenhancesfthefcontrastfbetweenfintracellularfstructuresfhavingfslightfd
ifferencesfinfthefrefractivefindex?f-fANSWERfffphase-contrastfmicroscope
Whichfmicroscopefshowsfafbrightfimagefoffthefobjectfresultingffromftheffluorescentflightfe
mittedfbyfthefspecimen?f-fANSWERffftheffluorescencefmicroscope
Theffluorescencefmicroscopefexposesfthefspecimenftofwhatftypesfofflight?f-
fANSWERfffuv,f violet,f orfblue
Thefdistancefbetweenfthefcenterfofftheflensfandffocalfpoint?f-fANSWERffffocalflength
Thefshorterftheffocalfpoint,fthef______fthefmagnificationf-fANSWERfffgreater
Thefshorterfthefwavelength,fthef______fthefresolutionf-fANSWERfffgreater
, Thefdistancefbetweenftheffrontfsurfacefofflensfandfsurfacefoffcoverfglassfonfspecimenf-
fANSWERfffWorkingfdistance
Asfthefworkingfdistancefdecrease,ftheffieldfoffviewf_____f-fANSWERfffdecrease
Asftheffieldfoffviewfdecrease,ftheflightfgetsf____f-fANSWERfffdimmer
Whatfisfthefpurposefoffaddingfthefimmersionfoil?f-
fANSWERffftofobtainfmoref lightfbyfprovidingf thefsamefrefractionfenergyf asf glass
Asfthefmagnificationfincreases,fwhatfhappensftoftheffocalflength?f-
fANSWERfffitfdecrease
Whatfisfthefchargefoffafbacteriafcellfsurface?f-fANSWERfffnegativefcharge
Whatfisfthefchargefoffbasicfdyes?f-fANSWERfffpositivefcharge
Whatfisfthefchargefoffacidfdyes?f-fANSWERfffnegativefcharge
Afthin,funiformffilmfoffbacterialfgrowthfonfafglassfslidefinforderftofproceedfwithffurtherfstain
ingfforfmicroscopicfexaminationf-fANSWERfffsmear
Thefprocessfbyfwhichforganismfisfkilledfandffirmlyfattachedftofmicroscopefslidef-
fANSWERffffixation
Heatffixationfvs.fChemicalffixingf(notfthefprocedure)f-fANSWERfff-
heatffixationfpreservesfoverallfshapefbutfnotfinternalfstructures
-
chemicalffixingfprotectsffinefcellularfstructurefandfshapefofflarger,fmorefdelicateforganis
ms
Whatfinformationfdoesfdifferentialfstainingfprovide?f-
fANSWERfffdividesfmicroorganismsf intof groupsfbasedf onf theirf stainingfproperties
Whatfisfthefmostfwidelyfusedfdifferentialfstainingfprocedure?f-fANSWERfffgramfstaining
Negativefstainingf-fANSWERfffthefbacteriafisfnotfstained;fthefbackgroundfisfstained
Whatfisfshadowingfinfelectronfmicroscopy?f-
fANSWERfffcoatingf specimenf withfaf thinffilmf offheavyf metal
WhatfisftheftotalfmagnificationfoffthefTransmissionfElectronfMicroscopefandfthefScannin
gfElectronfMicroscope?f-fANSWERfff1,000,000x
WhatfisftheftotalfmagnificationfoffthefScanningfElectronfMicroscope?f-
fANSWERfff1,000,000x
Whichfmicroscopefusesfelectronsfreflectedffromfthefsurfacefoffafspecimenftofcreatefanfi
mage?f-fANSWERfffscanningfelectronfmicroscope