MODERN PHARMACEUTICAL ANALYTICAL TECHNIQUES (Theory) (MPAT101T).

UNIT-1 a) UV-Visible spectroscopy: Introduction, Theory, Laws, Instrumentation associated with UV-Visible spectroscopy, Choice of solvents and solvent effect and Applications of UV Visible spectroscopy. b) IR spectroscopy: Theory, Modes of Molecular vibrations, Sample handling. Instrumentation of Dispersive and Fourier Transform IR Spectrometer, Factors affecting vibrational frequencies and Applications of IR spectroscopy, Data Interpretation. 10 hrs c) Spectroflourimetry: Theory of Fluorescence, Factors affecting fluorescence, Quenchers, Instrumentation and Applications of fluorescence spectroscopy. d) Flame emission spectroscopy and Atomic absorption spectroscopy: Principle, Instrumentation, Interferences and Applications. UNIT-II NMR spectroscopy: Quantum numbers and their role in NMR, Principle, Instrumentation, Solvent requirement in NMR. Relaxation process, NMR signals in various compounds, Chemical shift, Factors influencing chemical shift, Spin-Spin coupling, Coupling constant, Nuclear magnetic double resonance, Brief outline of principles of FT-NMR and 13C NMR. Applications of NMR spectroscopy. 10 hrs UNTI Mass Spectrometry: Principle, Theory, Instrumentation of Mass Spectrometry, Different types of ionization like electron impact, chemical, field, FAB and MALDI, APCI, ESI, APPI Analyzers of Quadrupole and Time of Flight, Mass fragmentation and its rules, Meta stable ions, Isotopic peaks and Applications of Mass spectrometry 12 hrs 10 hrs Simple structure elucidation problems based on UV, IR, NMR and Mass data. UNIT-IV Chromatography: Principle, instrumentation, chromatographic parameters, factors affecting resolution and applications of the following: a) High Performance Liquid chromatography b) High Performance Thin Layer Chromatography c) Ion exchange chromatography 34 d) Gas chromatography e) Ultra High Performance Liquid chromatography f) Affinity chromatography g) Gel Chromatography UNIT-V a) Electrophoresis: Principle, Instrumentation, Working conditions, factors affecting separation and applications of the following: a) Paper electrophoresis b) Gel electrophoresis c) Capillary electrophoresis d) Zone electrophoresis e) Moving boundary electrophoresis f) Iso electric focusing 10 hrs b) X ray Crystallography: Production of X rays, Different X ray diffraction methods, Bragg's law, Rotating crystal technique, X ray powder technique, Types of crystals and applications of X ray diffraction. UNIT - VI Thermal Techniques: a) Thermogravimetric analysis (TGA): Principle, instrumentation, factors affecting results, advantage and disadvantages, pharmaceutical applications. b) Differential scanning calorimetry (DSC): Principle, thermal transitions and Instrumentation (Heat flux and power-compensation and designs), Modulated DSC, Hyper DSC, experimental parameters (sample preparation, experimental conditions, calibration, heating and cooling rates, resolution, source of errors) and their influence, advantage and disadvantages, pharmaceutical applications. 08 hrs c) Differential Thermal Analysis (DTA): Principle, instrumentation and advantage and disadvantages, pharmaceutical applications, derivative differential thermal analysis (DDTA).