STUDENTS SUCCESS
Revision Examination Tests
“Come all for this Greatness”
... 100% Correct Ans ...
ASCP CG CERTIFICATION PRACTICE EXAM
2024-2025 ACTUAL EXAM COMPLETE 200-
QUESTIONS AND CORRECT DETAILED ANSWERS
What is the best agent to use against mycoplasma?
Ans: BM cyclin - a combination of two antibiotics (tiamulin and minocycline) that
effecticely targets mycoplasma growth
Quiz For successful harvest, each new batch of hypotonic solution should be
evaluated by:
Ans: comparing the new lot to a lot already in use
Quiz Colagenase, pronase, and trypsin are:
Ans: Proteolytic enzymes - break down tissues into single-cell suspensions
Quiz When harvesting peripheral blood cultures, asceptic technique no longer
matters following incubation with:
Ans: Colcemid - mitotic spindle poison that halts cells at metaphase
Quiz Samples that arrive at the cytogenetics lab should first be:
Ans: Logged in to the system
Quiz Phytohemagglutinin (PHA) stimulates which type of cells into division?
Ans: T-cell lymphocytes
Quiz To avoid culture failure, monitoring reagents is crucial. When logging a culture
failure, what is the MOST important to document?
Ans: Expiration dates of reagents used
,Quiz Which type of microscope is used when cleaning a CVS (chorionic villus)
sample?
Ans: Dissection
Quiz Which cell type is most problematic if found in a CVS (chorionic villus) sample?
Ans: Decidua (maternal tissue)
Quiz When looking at a slide under a phase contrast scope and a lot of cytoplasm is
still seen around the mets, what is the most likely cause?
Ans: innappropriate slide drying time
Quiz What will help prevent microbial contamination and cross contamination of
cultures?
Ans: Asceptic technique
Quiz When there are not enough mets to complete the study of a patient/case, what
needs to be done next?
Ans: prepare additional slides from any remaining sample
Quiz When looking at a dropped slide under a phase scope and mets are very
spread out and sparse, what could help fix the issue?
Ans: Increading the drying time by using warm slides
Quiz What is true of an inverted microscope?
Ans: the objectives are beneath the stage
Quiz High-resolution banding is indicated for a possible diagnosis of:
Ans: DiGenorge syndrome -- microdeletion of 22q11, also called velocardiofacial
syndrome
"velo" - soft palette,
"cardio" - congenital heart defects,
"facial" - unique abnormal facial features, short philtrum (upper lip indent), tubular
nose, wide spread eyes, etc
Quiz When verifying if a specimen is appropriate for cytogenetic analysis, what is the
MOST important data to accompany the specimen?
Ans: Reason for referral
Quiz Which type of specimen should be requested for a cytogenetic analysis for
turner syndrome?
, Ans: Heparinized peripheral blood stored at room temperature
(NOT FROZEN)
Quiz A blood sample can be used to study the cytogenetics of a malignancy only if:
Ans: Dividing leukemic cells are present in circulation
Quiz All of the cultures in a CO2 incubator failed, what aspects of the incubator are
important to monitor to assess the source of the problem?
Ans: Temperature,
CO2 level, and
Humidity level
Quiz The most important factor affecting the quality of chromosomes viewed under a
phase contrast scope is:
Ans: Slide drying after the cells have been dropped
Quiz What is the modal number (default number of chromosomes in a cell) for a
Down syndrome translocation carrier?
Ans: 45
Quiz What is the best tissue to use for both a good success rate and abnormality
detection in spontaneous abortion miscarriages?
Ans: Chorionic villi
Quiz What is a possible cause for a drying oven to not maintain a proper
temperature?
Ans: The door does not seal properly
Quiz If a tech with a cold and sneezing does not take proper precautions while
setting up amniotic fluid samples, the specimen could become contaminated with:
Ans: Mycoplasma
--> the most common contaminant by a sick tech and can be detected through a
specific stain used on the sample
mycoplasma can induce abnormalities in a sample, so it is important to avoid
contamination
Quiz What about the metaphases on a slide are affected by slide making?
Ans: Cell density - thru dilution of the sample with fix
Revision Examination Tests
“Come all for this Greatness”
... 100% Correct Ans ...
ASCP CG CERTIFICATION PRACTICE EXAM
2024-2025 ACTUAL EXAM COMPLETE 200-
QUESTIONS AND CORRECT DETAILED ANSWERS
What is the best agent to use against mycoplasma?
Ans: BM cyclin - a combination of two antibiotics (tiamulin and minocycline) that
effecticely targets mycoplasma growth
Quiz For successful harvest, each new batch of hypotonic solution should be
evaluated by:
Ans: comparing the new lot to a lot already in use
Quiz Colagenase, pronase, and trypsin are:
Ans: Proteolytic enzymes - break down tissues into single-cell suspensions
Quiz When harvesting peripheral blood cultures, asceptic technique no longer
matters following incubation with:
Ans: Colcemid - mitotic spindle poison that halts cells at metaphase
Quiz Samples that arrive at the cytogenetics lab should first be:
Ans: Logged in to the system
Quiz Phytohemagglutinin (PHA) stimulates which type of cells into division?
Ans: T-cell lymphocytes
Quiz To avoid culture failure, monitoring reagents is crucial. When logging a culture
failure, what is the MOST important to document?
Ans: Expiration dates of reagents used
,Quiz Which type of microscope is used when cleaning a CVS (chorionic villus)
sample?
Ans: Dissection
Quiz Which cell type is most problematic if found in a CVS (chorionic villus) sample?
Ans: Decidua (maternal tissue)
Quiz When looking at a slide under a phase contrast scope and a lot of cytoplasm is
still seen around the mets, what is the most likely cause?
Ans: innappropriate slide drying time
Quiz What will help prevent microbial contamination and cross contamination of
cultures?
Ans: Asceptic technique
Quiz When there are not enough mets to complete the study of a patient/case, what
needs to be done next?
Ans: prepare additional slides from any remaining sample
Quiz When looking at a dropped slide under a phase scope and mets are very
spread out and sparse, what could help fix the issue?
Ans: Increading the drying time by using warm slides
Quiz What is true of an inverted microscope?
Ans: the objectives are beneath the stage
Quiz High-resolution banding is indicated for a possible diagnosis of:
Ans: DiGenorge syndrome -- microdeletion of 22q11, also called velocardiofacial
syndrome
"velo" - soft palette,
"cardio" - congenital heart defects,
"facial" - unique abnormal facial features, short philtrum (upper lip indent), tubular
nose, wide spread eyes, etc
Quiz When verifying if a specimen is appropriate for cytogenetic analysis, what is the
MOST important data to accompany the specimen?
Ans: Reason for referral
Quiz Which type of specimen should be requested for a cytogenetic analysis for
turner syndrome?
, Ans: Heparinized peripheral blood stored at room temperature
(NOT FROZEN)
Quiz A blood sample can be used to study the cytogenetics of a malignancy only if:
Ans: Dividing leukemic cells are present in circulation
Quiz All of the cultures in a CO2 incubator failed, what aspects of the incubator are
important to monitor to assess the source of the problem?
Ans: Temperature,
CO2 level, and
Humidity level
Quiz The most important factor affecting the quality of chromosomes viewed under a
phase contrast scope is:
Ans: Slide drying after the cells have been dropped
Quiz What is the modal number (default number of chromosomes in a cell) for a
Down syndrome translocation carrier?
Ans: 45
Quiz What is the best tissue to use for both a good success rate and abnormality
detection in spontaneous abortion miscarriages?
Ans: Chorionic villi
Quiz What is a possible cause for a drying oven to not maintain a proper
temperature?
Ans: The door does not seal properly
Quiz If a tech with a cold and sneezing does not take proper precautions while
setting up amniotic fluid samples, the specimen could become contaminated with:
Ans: Mycoplasma
--> the most common contaminant by a sick tech and can be detected through a
specific stain used on the sample
mycoplasma can induce abnormalities in a sample, so it is important to avoid
contamination
Quiz What about the metaphases on a slide are affected by slide making?
Ans: Cell density - thru dilution of the sample with fix
