Genetics PCB 3063 (2025)
comprehensive questions and verified
answers ( detailed & elaborated)
ACTUAL EXAM 2025 TEST!!
The role of the primers in PCR is _______. -
✔✔ANSW✔✔to define the target region and
provide a 3' end that can be extended by taq
polymerase
If there are five molecules of DNA containing the
target region at the beginning of a PCR reaction,
how many copies of the target will be present after
three rounds of amplification? - ✔✔ANSW✔✔40
Immediately after the primers have annealed to the
target sequence, _______. - ✔✔ANSW✔✔the
temperature is raised so that taq polymerase can
extend the primers
,Which of the following molecules is required for a
PCR reaction? - ✔✔ANSW✔✔Primer, DNTPs, and
DNA
The thermostability of Taq polymerase is required
during the annealing phase of PCR. -
✔✔ANSW✔✔True
What is the purpose of raising the temperature to 90-
95°C at the beginning of each cycle of PCR? -
✔✔ANSW✔✔To separate the double-stranded
DNA
Which three steps constitute a single PCR cycle? -
✔✔ANSW✔✔denaturation, annealing, and
extension
The PCR (polymerase chain reaction) protocol that
is currently used in laboratories was facilitated by
the discovery of a bacterium called Thermus
aquaticus in a hot spring inside Yellowstone
National Park, in Wyoming. This organism contains
a heat-stable form of DNA polymerase known as
, Taq polymerase, which continues to function even
after it has been heated to 95°C. Why would such a
heat-stable polymerase be beneficial in PCR? -
✔✔ANSW✔✔Each cycle includes a "hot"
denaturation phase (95°C), which separates the
hydrogen bonds that hold the strands of the template
DNA together.
The products of restriction digestion can be
visualized by gel electrophoresis, which separates
fragments based on their size. - ✔✔ANSW✔✔true
A 1.5-kb fragment of DNA is cloned into a plasmid
vector that is 5.5 kb long at the EcoRI site, and the
plasmid vector is then used to transform bacteria. If
the plasmid DNA is then extracted from a single
bacterial colony and digested with EcoRI, what
digestion products will be produced if the plasmid
contains the fragment? - ✔✔ANSW✔✔One 1.5-kb
fragment and one 5.5-kb fragment
Digestion of a 1.1-kb fragment of DNA with BamHI
produces two fragments of 700 bp and 400 bp.
Digestion of the same 1.1-kb fragment with XhoI
produces two fragments of 300 bp and 800 bp.
comprehensive questions and verified
answers ( detailed & elaborated)
ACTUAL EXAM 2025 TEST!!
The role of the primers in PCR is _______. -
✔✔ANSW✔✔to define the target region and
provide a 3' end that can be extended by taq
polymerase
If there are five molecules of DNA containing the
target region at the beginning of a PCR reaction,
how many copies of the target will be present after
three rounds of amplification? - ✔✔ANSW✔✔40
Immediately after the primers have annealed to the
target sequence, _______. - ✔✔ANSW✔✔the
temperature is raised so that taq polymerase can
extend the primers
,Which of the following molecules is required for a
PCR reaction? - ✔✔ANSW✔✔Primer, DNTPs, and
DNA
The thermostability of Taq polymerase is required
during the annealing phase of PCR. -
✔✔ANSW✔✔True
What is the purpose of raising the temperature to 90-
95°C at the beginning of each cycle of PCR? -
✔✔ANSW✔✔To separate the double-stranded
DNA
Which three steps constitute a single PCR cycle? -
✔✔ANSW✔✔denaturation, annealing, and
extension
The PCR (polymerase chain reaction) protocol that
is currently used in laboratories was facilitated by
the discovery of a bacterium called Thermus
aquaticus in a hot spring inside Yellowstone
National Park, in Wyoming. This organism contains
a heat-stable form of DNA polymerase known as
, Taq polymerase, which continues to function even
after it has been heated to 95°C. Why would such a
heat-stable polymerase be beneficial in PCR? -
✔✔ANSW✔✔Each cycle includes a "hot"
denaturation phase (95°C), which separates the
hydrogen bonds that hold the strands of the template
DNA together.
The products of restriction digestion can be
visualized by gel electrophoresis, which separates
fragments based on their size. - ✔✔ANSW✔✔true
A 1.5-kb fragment of DNA is cloned into a plasmid
vector that is 5.5 kb long at the EcoRI site, and the
plasmid vector is then used to transform bacteria. If
the plasmid DNA is then extracted from a single
bacterial colony and digested with EcoRI, what
digestion products will be produced if the plasmid
contains the fragment? - ✔✔ANSW✔✔One 1.5-kb
fragment and one 5.5-kb fragment
Digestion of a 1.1-kb fragment of DNA with BamHI
produces two fragments of 700 bp and 400 bp.
Digestion of the same 1.1-kb fragment with XhoI
produces two fragments of 300 bp and 800 bp.
