BIOL3200 MEHARI EXAM 2 QUESTIONS AND ANSWERS
WITH COMPLETE SOLUTIONS GRADED A++ LATEST
UPDATE
simple diffusion
diffusion of molecules
passive transport
does not require energy
Examples of passive transport
Simple & facilitated diffusion
active transport
requires energy
What type of transport is coupled transport?
active
coupled transport
energy released by moving driving ion down a gradient and a solute up
What are the two kinds of coupled transport
Symport and Antiport
Symport
2 molecule travel same direction
antiport
,transporter molecule moves in opposite direction of driving ion
True or False: ABC Transporters are found in only prokaryotes
FALSE: ABC Transporters are found in EVERY living thing
What do ABC transporters do?
Import nutrient
Export drugs
largest family
what are the two main types of ABC transporters?
uptake (transport nutrients)
efflux (multidrug pumps)
facilitated diffusion
helps solutes move across membrane from high to low conc. but CAN NOT move
molecules
sideophoers
specialized molecules secreted to bind ferric ion & transport into cell
pure culture
where microbes grow separately from other
What are liquid/broth for?
studying growth characteristics of pure culture
What are solid/ Agar gel plates for?
separate mixed cultures from other environments
How are pure colonies isolated?
,dilution streaking
spread plate
tenfold serial dilutions
What are the 5 diff. types of media?
Complex
Synthetic
Enriched
Selective
Differential
Complex media
nutrient rich but poorly defined
synthetic media
precisely defined
enriched media
complex media w/ specific blood components
ex: fastidious (req. blood)
selective media
favor growth of 1 organism over the other
differential media
exploit difference between 2 species
Petroff-Hausser counting chamber
microbes counted by directly placing dilution under slide coverslip
Fluorescence Activated Cell Sorter
, fluorescent cells passed through laser & counted from lighter scatter
viable bacterium
being capable of replication & colony forming
What is the ideal range for CFUs
30-300
binary fission
symmetrical division
growth rate
rate of inc. cell numbers
generation time
time takes population to double
batch culture
way to model effects of changing environment on bacteria (liquid medium)
lag phase
(1) making molecules & lipids & sugar, prep for division
log phase
(2) exponential growth
stationary phase
(3) growth limited to resources & accumulation of toxins (products of growth)
What stage do cells die with half life (radioactive decay)
4
continuos culture
cells populate @ steady rate
WITH COMPLETE SOLUTIONS GRADED A++ LATEST
UPDATE
simple diffusion
diffusion of molecules
passive transport
does not require energy
Examples of passive transport
Simple & facilitated diffusion
active transport
requires energy
What type of transport is coupled transport?
active
coupled transport
energy released by moving driving ion down a gradient and a solute up
What are the two kinds of coupled transport
Symport and Antiport
Symport
2 molecule travel same direction
antiport
,transporter molecule moves in opposite direction of driving ion
True or False: ABC Transporters are found in only prokaryotes
FALSE: ABC Transporters are found in EVERY living thing
What do ABC transporters do?
Import nutrient
Export drugs
largest family
what are the two main types of ABC transporters?
uptake (transport nutrients)
efflux (multidrug pumps)
facilitated diffusion
helps solutes move across membrane from high to low conc. but CAN NOT move
molecules
sideophoers
specialized molecules secreted to bind ferric ion & transport into cell
pure culture
where microbes grow separately from other
What are liquid/broth for?
studying growth characteristics of pure culture
What are solid/ Agar gel plates for?
separate mixed cultures from other environments
How are pure colonies isolated?
,dilution streaking
spread plate
tenfold serial dilutions
What are the 5 diff. types of media?
Complex
Synthetic
Enriched
Selective
Differential
Complex media
nutrient rich but poorly defined
synthetic media
precisely defined
enriched media
complex media w/ specific blood components
ex: fastidious (req. blood)
selective media
favor growth of 1 organism over the other
differential media
exploit difference between 2 species
Petroff-Hausser counting chamber
microbes counted by directly placing dilution under slide coverslip
Fluorescence Activated Cell Sorter
, fluorescent cells passed through laser & counted from lighter scatter
viable bacterium
being capable of replication & colony forming
What is the ideal range for CFUs
30-300
binary fission
symmetrical division
growth rate
rate of inc. cell numbers
generation time
time takes population to double
batch culture
way to model effects of changing environment on bacteria (liquid medium)
lag phase
(1) making molecules & lipids & sugar, prep for division
log phase
(2) exponential growth
stationary phase
(3) growth limited to resources & accumulation of toxins (products of growth)
What stage do cells die with half life (radioactive decay)
4
continuos culture
cells populate @ steady rate
