BIOL340 - REPLICATION #1 EXAM QUESTIONS AND ANSWERS
WITH COMPLETE SOLUTIONS VERIFIED LATEST UPDATE
What are the three activities associated with DNA replication?
Initiation, Elongation, Termination
What is Initiation?
Recognition of an origin, separation and stabilization of parental strands.
What is Elongation?
Assembly of the replisome at the replication fork, synthesis of the daughter strands.
What is Termination?
Joining at the ends, separation of the chromosome.
What is the origin of replication in E. coli?
A 245 base pair sequence called OriC.
What is the function and gene associated with DNA polymerase I?
DNA replication, polA.
What is the function and gene associated with DNA polymerase II?
Unknown, polB.
What is the function and gene associated with DNA polymerase III?
DNA replication, dnaeE/Q/X/N/D, holA-E.
What is the function and gene associated with Initiator protein?
Binds to OriC, dnaA.
What is the function and gene associated with DNA helicase?
, Helicase & activator of primase, dnaB.
What is the function and gene associated with Primase?
Makes RNA primer for extension by pol III, dnaG.
What is the function and gene associated with DNA ligase?
Seals single stranded gaps, lig.
What is the function and gene associated with Single Stranded Binding Protein
(SSB)?
Binds unwound single stranded DNA, ssb.
What is the function and gene associated with DNA gyrase? (topoisomerase II)
Replication swivel to stop DNA tangling, gyrA/B.
What is the function and gene associated with origin?
Origin of replication, oriC.
What is the function and gene associated with terminus of replication?
Ter.
What is the function and gene associated with Ter binding protein (TBP)?
Stalls replication fork, tus.
Which step of initiation involves initiator proteins binding to replication origin,
requiring topoisomerase to relax DNA, so that the DNA can bind around the
initiator to form the complex.
Step 1.
What is step 1 of initiation?
Initiator proteins bind to replication origin. Requires relaxing by topoisomerase.
Which step of initiation involves DNA helicase binding to initiator proteins?
WITH COMPLETE SOLUTIONS VERIFIED LATEST UPDATE
What are the three activities associated with DNA replication?
Initiation, Elongation, Termination
What is Initiation?
Recognition of an origin, separation and stabilization of parental strands.
What is Elongation?
Assembly of the replisome at the replication fork, synthesis of the daughter strands.
What is Termination?
Joining at the ends, separation of the chromosome.
What is the origin of replication in E. coli?
A 245 base pair sequence called OriC.
What is the function and gene associated with DNA polymerase I?
DNA replication, polA.
What is the function and gene associated with DNA polymerase II?
Unknown, polB.
What is the function and gene associated with DNA polymerase III?
DNA replication, dnaeE/Q/X/N/D, holA-E.
What is the function and gene associated with Initiator protein?
Binds to OriC, dnaA.
What is the function and gene associated with DNA helicase?
, Helicase & activator of primase, dnaB.
What is the function and gene associated with Primase?
Makes RNA primer for extension by pol III, dnaG.
What is the function and gene associated with DNA ligase?
Seals single stranded gaps, lig.
What is the function and gene associated with Single Stranded Binding Protein
(SSB)?
Binds unwound single stranded DNA, ssb.
What is the function and gene associated with DNA gyrase? (topoisomerase II)
Replication swivel to stop DNA tangling, gyrA/B.
What is the function and gene associated with origin?
Origin of replication, oriC.
What is the function and gene associated with terminus of replication?
Ter.
What is the function and gene associated with Ter binding protein (TBP)?
Stalls replication fork, tus.
Which step of initiation involves initiator proteins binding to replication origin,
requiring topoisomerase to relax DNA, so that the DNA can bind around the
initiator to form the complex.
Step 1.
What is step 1 of initiation?
Initiator proteins bind to replication origin. Requires relaxing by topoisomerase.
Which step of initiation involves DNA helicase binding to initiator proteins?
