BIOL4004 WEEK 2 EXAM QUESTIONS AND ANSWERS
WITH COMPLETE SOLUTIONS VERIFIED
Hybridoma cell line
A fusion of a B lymphocyte and a tumor cell that produces unlimited quantities of a
particular antibody
In vitro
Experiments carried out using cultured cells in a dish ("in glass")
Column chromatography
General term for a purification technique that separates proteins by passing over a
matrix-filled column
Gel filtration chromatography
Use of this technique based on size
Affinity chromatography
Use of this technique based on a protein interaction
Ion exchange chromatography
Use of this technique based on charge
Restriction nuclease
An enzyme that cleaves double stranded DNA at a specific short palindromic nucleotide
sequence
Probe
WITH COMPLETE SOLUTIONS VERIFIED
Hybridoma cell line
A fusion of a B lymphocyte and a tumor cell that produces unlimited quantities of a
particular antibody
In vitro
Experiments carried out using cultured cells in a dish ("in glass")
Column chromatography
General term for a purification technique that separates proteins by passing over a
matrix-filled column
Gel filtration chromatography
Use of this technique based on size
Affinity chromatography
Use of this technique based on a protein interaction
Ion exchange chromatography
Use of this technique based on charge
Restriction nuclease
An enzyme that cleaves double stranded DNA at a specific short palindromic nucleotide
sequence
Probe
