BIOL4004 VISUALIZING CELLS EXAM QUESTIONS AND
ANSWERS WITH COMPLETE SOLUTIONS VERIFIED
LATEST UPDATE
What are light microscopes formed by?
The interaction of light with tissue components
What are electron microscopes formed by?
The interaction of electrons with tissue components
Definition of detection
Ability to perceive (detect) the presence of an object
Definition of resolution
The ability to discriminate between (resolve) two objects that are close together
Definition of limit of resolution
The smallest distance at which two objects can be observed as being separate entities
Limit of resolution of the unaided eye
250 microns
Limit of resolution of a light microscope
0.25 microns
Limit of resolution of an electron microscope
0.001 microns (1 nm)
Memorize the limit of resolution equation:
, What does λ stand for?
wavelength of illumination
What does N.A. stand for?
Numerical aperture, or quality of lens.
It indicates the light gathering ability of a lens.
What does a higher N.A. signify?
Greater resolution and brighter image
Definition of wavelength
Distance between wave peaks
Function of the condenser lens of a microscope
Focuses a cone of light rays onto each point of the specimen
Function of the objective lens of a microscope
Collects a cone of light rays to create an image
Definition of magnification
The image size relative to the object size
What is the relationship between magnification and resolution?
The higher the resolution, the greater the useful magnification
Definition of empty magnification
Magnification which yields no further useful information or resolution and only degrades
the image
Compound light microscope means:
Multiple lenses
What path does light follow in a compound light microscope?
ANSWERS WITH COMPLETE SOLUTIONS VERIFIED
LATEST UPDATE
What are light microscopes formed by?
The interaction of light with tissue components
What are electron microscopes formed by?
The interaction of electrons with tissue components
Definition of detection
Ability to perceive (detect) the presence of an object
Definition of resolution
The ability to discriminate between (resolve) two objects that are close together
Definition of limit of resolution
The smallest distance at which two objects can be observed as being separate entities
Limit of resolution of the unaided eye
250 microns
Limit of resolution of a light microscope
0.25 microns
Limit of resolution of an electron microscope
0.001 microns (1 nm)
Memorize the limit of resolution equation:
, What does λ stand for?
wavelength of illumination
What does N.A. stand for?
Numerical aperture, or quality of lens.
It indicates the light gathering ability of a lens.
What does a higher N.A. signify?
Greater resolution and brighter image
Definition of wavelength
Distance between wave peaks
Function of the condenser lens of a microscope
Focuses a cone of light rays onto each point of the specimen
Function of the objective lens of a microscope
Collects a cone of light rays to create an image
Definition of magnification
The image size relative to the object size
What is the relationship between magnification and resolution?
The higher the resolution, the greater the useful magnification
Definition of empty magnification
Magnification which yields no further useful information or resolution and only degrades
the image
Compound light microscope means:
Multiple lenses
What path does light follow in a compound light microscope?
